Identification of Cronobacter in infant formula using real-time polymerase chain reaction
ObjectiveTo establish a real-time quantitative polymerase chain reaction （PCR） method for rapid and accurate identification of Cronobacter spp. in food samples and artificially contaminated samples.MethodsPrimers and probes were designed based on the conserved region of gyrB of Cronobacter spp. DNA....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2023-06-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202306006 |
| _version_ | 1827713872424861696 |
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| author | WANG Qinglong ZHANG Yuechuan ZHOU Yanxia GONG Youbo LI Shuang SHI Jinshuo DING Shanshan RAN Linglei WANG Kaiyi TANG Yuhan CAI Xuefeng |
| author_facet | WANG Qinglong ZHANG Yuechuan ZHOU Yanxia GONG Youbo LI Shuang SHI Jinshuo DING Shanshan RAN Linglei WANG Kaiyi TANG Yuhan CAI Xuefeng |
| author_sort | WANG Qinglong |
| collection | DOAJ |
| description | ObjectiveTo establish a real-time quantitative polymerase chain reaction （PCR） method for rapid and accurate identification of Cronobacter spp. in food samples and artificially contaminated samples.MethodsPrimers and probes were designed based on the conserved region of gyrB of Cronobacter spp. DNA. The method was verified using a specificity test， absolute sensitivity test， relative sensitivity test， and anti-interference test. Detection sensitivity was determined using artificially contaminated samples.ResultsThe method established in this study could specifically amplify seven kinds of Cronobacter spp.， but not the other Enterobacter species closely related to it and other pathogens common in food， suggesting that this method has good anti-interference ability. The absolute sensitivity was 1-10 pg and the relative sensitivity was 103 CFU/mL using Cronobacter sakazakii. It had good anti-interference ability at the genome and culture level. The sensitivity of artificially contaminated samples could reach 100 CFU/mL after incubation at 36 ℃ for 24 h.ConclusionThe real-time PCR method developed in this study is rapid， specific， sensitive， and stable for the detection of Cronobacter spp. in infant formula food samples and can provide technical reference for the detection of Cronobacter spp. in infant formula food. |
| first_indexed | 2024-03-10T18:45:58Z |
| format | Article |
| id | doaj.art-ee842a95ec384a078a17b7db86372e8c |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-10T18:45:58Z |
| publishDate | 2023-06-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-ee842a95ec384a078a17b7db86372e8c2023-11-20T05:33:38ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562023-06-0135683684210.13590/j.cjfh.2023.06.0061004-8456（2023）06-0836-07Identification of Cronobacter in infant formula using real-time polymerase chain reactionWANG QinglongZHANG YuechuanZHOU YanxiaGONG YouboLI ShuangSHI JinshuoDING ShanshanRAN LingleiWANG KaiyiTANG YuhanCAI XuefengObjectiveTo establish a real-time quantitative polymerase chain reaction （PCR） method for rapid and accurate identification of Cronobacter spp. in food samples and artificially contaminated samples.MethodsPrimers and probes were designed based on the conserved region of gyrB of Cronobacter spp. DNA. The method was verified using a specificity test， absolute sensitivity test， relative sensitivity test， and anti-interference test. Detection sensitivity was determined using artificially contaminated samples.ResultsThe method established in this study could specifically amplify seven kinds of Cronobacter spp.， but not the other Enterobacter species closely related to it and other pathogens common in food， suggesting that this method has good anti-interference ability. The absolute sensitivity was 1-10 pg and the relative sensitivity was 103 CFU/mL using Cronobacter sakazakii. It had good anti-interference ability at the genome and culture level. The sensitivity of artificially contaminated samples could reach 100 CFU/mL after incubation at 36 ℃ for 24 h.ConclusionThe real-time PCR method developed in this study is rapid， specific， sensitive， and stable for the detection of Cronobacter spp. in infant formula food samples and can provide technical reference for the detection of Cronobacter spp. in infant formula food.http://www.zgspws.com/zgspwszz/article/abstract/202306006real-time polymerase chain reactioncronobacter sakazakiigyrb gene |
| spellingShingle | WANG Qinglong ZHANG Yuechuan ZHOU Yanxia GONG Youbo LI Shuang SHI Jinshuo DING Shanshan RAN Linglei WANG Kaiyi TANG Yuhan CAI Xuefeng Identification of Cronobacter in infant formula using real-time polymerase chain reaction Zhongguo shipin weisheng zazhi real-time polymerase chain reaction cronobacter sakazakii gyrb gene |
| title | Identification of Cronobacter in infant formula using real-time polymerase chain reaction |
| title_full | Identification of Cronobacter in infant formula using real-time polymerase chain reaction |
| title_fullStr | Identification of Cronobacter in infant formula using real-time polymerase chain reaction |
| title_full_unstemmed | Identification of Cronobacter in infant formula using real-time polymerase chain reaction |
| title_short | Identification of Cronobacter in infant formula using real-time polymerase chain reaction |
| title_sort | identification of cronobacter in infant formula using real time polymerase chain reaction |
| topic | real-time polymerase chain reaction cronobacter sakazakii gyrb gene |
| url | http://www.zgspws.com/zgspwszz/article/abstract/202306006 |
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