Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative Transcriptomics
<i>Akkermansia muciniphila</i> is a common member of the human gut microbiota and belongs to the phylum Verrucomicrobia. Decreased levels of <i>A. muciniphila</i> are associated with many diseases, so it is thought to be a beneficial resident of the intestinal mucosal layer....
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MDPI AG
2023-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/3/440 |
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| author | Erna Li Shipei Li Fan Liu Qian Li Daorui Pang Hong Wang Sentai Liao Yuxiao Zou |
| author_facet | Erna Li Shipei Li Fan Liu Qian Li Daorui Pang Hong Wang Sentai Liao Yuxiao Zou |
| author_sort | Erna Li |
| collection | DOAJ |
| description | <i>Akkermansia muciniphila</i> is a common member of the human gut microbiota and belongs to the phylum Verrucomicrobia. Decreased levels of <i>A. muciniphila</i> are associated with many diseases, so it is thought to be a beneficial resident of the intestinal mucosal layer. In this study, we found that different prebiotics promoted the proliferation of <i>A. muciniphila</i>, and mulberry galacto-oligosaccharide (MGO) had the greatest effect. We cultured <i>A. muciniphila</i> in a brian heart infusion (BHI) medium containing 5% galactooligosaccharides (GOS), mulberry polysaccharide solution (MPS), and MGO, and transcriptomic analyses were performed. The results revealed that, after 6 days of cultivation, the numbers of upregulated functional genes (based on Gene Ontology) were approximately 0.7 and 19% higher with MPS and MGO, respectively, than with GOS. Analysis using the Kyoto Encyclopedia of Genes and Genomes showed that, when <i>A. muciniphila</i> was cultured with MGO, genes that were upregulated were enriched in the carbohydrate metabolism, the metabolism of cofactors and vitamins, the energy metabolism, the amino acid metabolism, and the lipid metabolism. Upregulated genes included <i>galM</i> and <i>pfkA</i> in the galactose metabolism, and <i>pgi</i>, <i>pfk</i>, <i>fbaA</i>, <i>tpiA</i>, <i>gapA</i>, <i>pgk</i>, <i>gpml</i>, <i>eno</i>, <i>pyk</i>, and <i>lpd</i> in the glycolysis/gluconeogenesis pathway. Real-time quantitative PCR results were consistent with the RNA-Seq data. This work provides valuable knowledge which can be available for the functional application of <i>A. muciniphila</i> and MGO. |
| first_indexed | 2024-03-11T09:45:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T09:45:34Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ee8464caba434c1f903e3d690779d3362023-11-16T16:39:23ZengMDPI AGFoods2304-81582023-01-0112344010.3390/foods12030440Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative TranscriptomicsErna Li0Shipei Li1Fan Liu2Qian Li3Daorui Pang4Hong Wang5Sentai Liao6Yuxiao Zou7Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China<i>Akkermansia muciniphila</i> is a common member of the human gut microbiota and belongs to the phylum Verrucomicrobia. Decreased levels of <i>A. muciniphila</i> are associated with many diseases, so it is thought to be a beneficial resident of the intestinal mucosal layer. In this study, we found that different prebiotics promoted the proliferation of <i>A. muciniphila</i>, and mulberry galacto-oligosaccharide (MGO) had the greatest effect. We cultured <i>A. muciniphila</i> in a brian heart infusion (BHI) medium containing 5% galactooligosaccharides (GOS), mulberry polysaccharide solution (MPS), and MGO, and transcriptomic analyses were performed. The results revealed that, after 6 days of cultivation, the numbers of upregulated functional genes (based on Gene Ontology) were approximately 0.7 and 19% higher with MPS and MGO, respectively, than with GOS. Analysis using the Kyoto Encyclopedia of Genes and Genomes showed that, when <i>A. muciniphila</i> was cultured with MGO, genes that were upregulated were enriched in the carbohydrate metabolism, the metabolism of cofactors and vitamins, the energy metabolism, the amino acid metabolism, and the lipid metabolism. Upregulated genes included <i>galM</i> and <i>pfkA</i> in the galactose metabolism, and <i>pgi</i>, <i>pfk</i>, <i>fbaA</i>, <i>tpiA</i>, <i>gapA</i>, <i>pgk</i>, <i>gpml</i>, <i>eno</i>, <i>pyk</i>, and <i>lpd</i> in the glycolysis/gluconeogenesis pathway. Real-time quantitative PCR results were consistent with the RNA-Seq data. This work provides valuable knowledge which can be available for the functional application of <i>A. muciniphila</i> and MGO.https://www.mdpi.com/2304-8158/12/3/440transcriptomics<i>Akkermansia muciniphila</i>mulberry galacto-oligosaccharidemulberry polysaccharideprebiotics |
| spellingShingle | Erna Li Shipei Li Fan Liu Qian Li Daorui Pang Hong Wang Sentai Liao Yuxiao Zou Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative Transcriptomics Foods transcriptomics <i>Akkermansia muciniphila</i> mulberry galacto-oligosaccharide mulberry polysaccharide prebiotics |
| title | Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative Transcriptomics |
| title_full | Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative Transcriptomics |
| title_fullStr | Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative Transcriptomics |
| title_full_unstemmed | Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative Transcriptomics |
| title_short | Analysis of <i>Akkermansia muciniphila</i> in Mulberry Galacto-Oligosaccharide Medium via Comparative Transcriptomics |
| title_sort | analysis of i akkermansia muciniphila i in mulberry galacto oligosaccharide medium via comparative transcriptomics |
| topic | transcriptomics <i>Akkermansia muciniphila</i> mulberry galacto-oligosaccharide mulberry polysaccharide prebiotics |
| url | https://www.mdpi.com/2304-8158/12/3/440 |
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