Effect of PACAP on Heat Exposure
Heat stroke is a life-threatening illness caused by exposure to high ambient temperatures and relative humidity. The incidence of heat stroke is expected to increase due to climate change. Although pituitary adenylate cyclase-activating polypeptide (PACAP) has been implicated in thermoregulation, th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3992 |
| _version_ | 1797620437529657344 |
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| author | Keisuke Suzuki Hiroki Yamaga Hirokazu Ohtaki Satoshi Hirako Kazuyuki Miyamoto Motoyasu Nakamura Kaoru Yanagisawa Takuya Shimada Tomohiko Hosono Hitoshi Hashimoto Kazuho Honda Kenji Dohi |
| author_facet | Keisuke Suzuki Hiroki Yamaga Hirokazu Ohtaki Satoshi Hirako Kazuyuki Miyamoto Motoyasu Nakamura Kaoru Yanagisawa Takuya Shimada Tomohiko Hosono Hitoshi Hashimoto Kazuho Honda Kenji Dohi |
| author_sort | Keisuke Suzuki |
| collection | DOAJ |
| description | Heat stroke is a life-threatening illness caused by exposure to high ambient temperatures and relative humidity. The incidence of heat stroke is expected to increase due to climate change. Although pituitary adenylate cyclase-activating polypeptide (PACAP) has been implicated in thermoregulation, the role of PACAP on heat stress remains unclear. PACAP knockout (KO) and wild-type ICR mice were subjected to heat exposure at an ambient temperature of 36 °C and relative humidity of 99% for 30–150 min. After heat exposure, the PACAP KO mice had a greater survival rate and maintained a lower body temperature than the wild-type mice. Moreover, the gene expression and immunoreaction of c-Fos in the ventromedially preoptic area of the hypothalamus, which is known to harbor temperature-sensitive neurons, were significantly lower in PACAP KO mice than those in wild-type mice. In addition, differences were observed in the brown adipose tissue, the primary site of heat production, between PACAP KO and wild-type mice. These results suggest that PACAP KO mice are resistant to heat exposure. The heat production mechanism differs between PACAP KO and wild-type mice. |
| first_indexed | 2024-03-11T08:41:26Z |
| format | Article |
| id | doaj.art-ee8ab164f9cd46cfaa3d38385d818b09 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:41:26Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ee8ab164f9cd46cfaa3d38385d818b092023-11-16T21:08:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244399210.3390/ijms24043992Effect of PACAP on Heat ExposureKeisuke Suzuki0Hiroki Yamaga1Hirokazu Ohtaki2Satoshi Hirako3Kazuyuki Miyamoto4Motoyasu Nakamura5Kaoru Yanagisawa6Takuya Shimada7Tomohiko Hosono8Hitoshi Hashimoto9Kazuho Honda10Kenji Dohi11Department of Emergency and Disaster Medicine, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Emergency and Disaster Medicine, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Anatomy, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Health and Nutrition, University of Human Arts and Sciences, Saitama 339-0077, JapanDepartment of Emergency and Disaster Medicine, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Emergency and Disaster Medicine, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Emergency and Disaster Medicine, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Emergency and Disaster Medicine, School of Medicine, Showa University, Tokyo 142-8555, JapanCenter for Biotechnology, Showa University, Tokyo 142-8555, JapanLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, JapanDepartment of Anatomy, School of Medicine, Showa University, Tokyo 142-8555, JapanDepartment of Emergency and Disaster Medicine, School of Medicine, Showa University, Tokyo 142-8555, JapanHeat stroke is a life-threatening illness caused by exposure to high ambient temperatures and relative humidity. The incidence of heat stroke is expected to increase due to climate change. Although pituitary adenylate cyclase-activating polypeptide (PACAP) has been implicated in thermoregulation, the role of PACAP on heat stress remains unclear. PACAP knockout (KO) and wild-type ICR mice were subjected to heat exposure at an ambient temperature of 36 °C and relative humidity of 99% for 30–150 min. After heat exposure, the PACAP KO mice had a greater survival rate and maintained a lower body temperature than the wild-type mice. Moreover, the gene expression and immunoreaction of c-Fos in the ventromedially preoptic area of the hypothalamus, which is known to harbor temperature-sensitive neurons, were significantly lower in PACAP KO mice than those in wild-type mice. In addition, differences were observed in the brown adipose tissue, the primary site of heat production, between PACAP KO and wild-type mice. These results suggest that PACAP KO mice are resistant to heat exposure. The heat production mechanism differs between PACAP KO and wild-type mice.https://www.mdpi.com/1422-0067/24/4/3992heat strokePACAPhypothalamuscore body temperature |
| spellingShingle | Keisuke Suzuki Hiroki Yamaga Hirokazu Ohtaki Satoshi Hirako Kazuyuki Miyamoto Motoyasu Nakamura Kaoru Yanagisawa Takuya Shimada Tomohiko Hosono Hitoshi Hashimoto Kazuho Honda Kenji Dohi Effect of PACAP on Heat Exposure International Journal of Molecular Sciences heat stroke PACAP hypothalamus core body temperature |
| title | Effect of PACAP on Heat Exposure |
| title_full | Effect of PACAP on Heat Exposure |
| title_fullStr | Effect of PACAP on Heat Exposure |
| title_full_unstemmed | Effect of PACAP on Heat Exposure |
| title_short | Effect of PACAP on Heat Exposure |
| title_sort | effect of pacap on heat exposure |
| topic | heat stroke PACAP hypothalamus core body temperature |
| url | https://www.mdpi.com/1422-0067/24/4/3992 |
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