A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
Abstract Neuronal activity is established as a driver of oligodendrocyte (OL) differentiation and myelination. The concept of activity-dependent myelin plasticity, and its role in cognition and disease, is gaining support. Methods capable of resolving changes in the morphology of individual myelinat...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-88887-0 |
| _version_ | 1830415236756144128 |
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| author | Eszter Toth Sayed Muhammed Rassul Martin Berry Daniel Fulton |
| author_facet | Eszter Toth Sayed Muhammed Rassul Martin Berry Daniel Fulton |
| author_sort | Eszter Toth |
| collection | DOAJ |
| description | Abstract Neuronal activity is established as a driver of oligodendrocyte (OL) differentiation and myelination. The concept of activity-dependent myelin plasticity, and its role in cognition and disease, is gaining support. Methods capable of resolving changes in the morphology of individual myelinating OL would advance our understanding of myelin plasticity and injury, thus we adapted a labelling approach involving Semliki Forest Virus (SFV) vectors to resolve and quantify the 3-D structure of OL processes and internodes in cerebellar slice cultures. We first demonstrate the utility of the approach by studying changes in OL morphology after complement-mediated injury. SFV vectors injected into cerebellar white matter labelled transitional OL (TOL), whose characteristic mixture of myelinating and non-myelinating processes exhibited significant degeneration after complement injury. The method was also capable of resolving finer changes in morphology related to neuronal activity. Prolonged suppression of neuronal activity, which reduced myelination, selectively decreased the length of putative internodes, and the proportion of process branches that supported them, while leaving other features of process morphology unaltered. Overall this work provides novel information on the morphology of TOL, and their response to conditions that alter circuit function or induce demyelination. |
| first_indexed | 2024-12-20T21:00:39Z |
| format | Article |
| id | doaj.art-ee8adcc82dcb4e94a938ef11d7709eb1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:00:39Z |
| publishDate | 2021-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ee8adcc82dcb4e94a938ef11d7709eb12022-12-21T19:26:43ZengNature PortfolioScientific Reports2045-23222021-05-0111111410.1038/s41598-021-88887-0A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytesEszter Toth0Sayed Muhammed Rassul1Martin Berry2Daniel Fulton3Neuroscience and Ophthalmology Research Group, Institute of Inflammation and Ageing, College of Medical and Dental Sciences, University of BirminghamNeuroscience and Ophthalmology Research Group, Institute of Inflammation and Ageing, College of Medical and Dental Sciences, University of BirminghamNeuroscience and Ophthalmology Research Group, Institute of Inflammation and Ageing, College of Medical and Dental Sciences, University of BirminghamNeuroscience and Ophthalmology Research Group, Institute of Inflammation and Ageing, College of Medical and Dental Sciences, University of BirminghamAbstract Neuronal activity is established as a driver of oligodendrocyte (OL) differentiation and myelination. The concept of activity-dependent myelin plasticity, and its role in cognition and disease, is gaining support. Methods capable of resolving changes in the morphology of individual myelinating OL would advance our understanding of myelin plasticity and injury, thus we adapted a labelling approach involving Semliki Forest Virus (SFV) vectors to resolve and quantify the 3-D structure of OL processes and internodes in cerebellar slice cultures. We first demonstrate the utility of the approach by studying changes in OL morphology after complement-mediated injury. SFV vectors injected into cerebellar white matter labelled transitional OL (TOL), whose characteristic mixture of myelinating and non-myelinating processes exhibited significant degeneration after complement injury. The method was also capable of resolving finer changes in morphology related to neuronal activity. Prolonged suppression of neuronal activity, which reduced myelination, selectively decreased the length of putative internodes, and the proportion of process branches that supported them, while leaving other features of process morphology unaltered. Overall this work provides novel information on the morphology of TOL, and their response to conditions that alter circuit function or induce demyelination.https://doi.org/10.1038/s41598-021-88887-0 |
| spellingShingle | Eszter Toth Sayed Muhammed Rassul Martin Berry Daniel Fulton A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes Scientific Reports |
| title | A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes |
| title_full | A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes |
| title_fullStr | A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes |
| title_full_unstemmed | A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes |
| title_short | A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes |
| title_sort | morphological analysis of activity dependent myelination and myelin injury in transitional oligodendrocytes |
| url | https://doi.org/10.1038/s41598-021-88887-0 |
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