Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus
Background: The origin of autoimmune disease type 1 diabetes is still unknown. Aim: This study assessed the activation of CD4+ and CD8+ T-lymphocytes by human insulin and human glutamate decarboxylase (GAD) in patients with type 1 or type 2 diabetes mellitus (DM) and healthy volunteers. Materials...
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| Format: | Article |
| Language: | English |
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Bioscientifica
2017-11-01
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| Series: | Endocrine Connections |
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| Online Access: | http://www.endocrineconnections.com/content/6/8/758.full |
| _version_ | 1828171175993278464 |
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| author | Borros Arneth |
| author_facet | Borros Arneth |
| author_sort | Borros Arneth |
| collection | DOAJ |
| description | Background: The origin of autoimmune disease type 1 diabetes is still unknown.
Aim: This study assessed the activation of CD4+ and CD8+ T-lymphocytes by human insulin and human glutamate decarboxylase (GAD) in patients with type 1 or type 2 diabetes mellitus (DM) and healthy volunteers.
Materials and methods: The expression of CD69, a marker of T-lymphocyte activity, was determined in whole blood samples by flow cytometry after 12 h of incubation with or without insulin or GAD. The analysis included samples from 12 type 1 DM patients, 14 type 2 DM patients and 12 healthy volunteers.
Results: Significant increases in the number of activated CD4+ and CD8+ T-lymphocytes following pre-incubation of whole blood samples with human insulin or GAD were observed in samples from patients with type 1 DM, whereas no activation of these cells was detected in samples from either type 2 DM patients or healthy subjects.
Discussion: These results indicated that latent pre-activation of CD4+ and CD8+ T-lymphocytes in response to insulin or GAD epitopes occurred in type 1 DM patients.
Conclusion: These findings suggest that pre-immunization against insulin and/or GAD might be associated with the development of type 1 DM. Alternatively, these results might reflect a non-specific, bystander autoimmune response. |
| first_indexed | 2024-04-12T03:23:27Z |
| format | Article |
| id | doaj.art-ee8b2738bb544d25831fca7ffa5e67e0 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 2049-3614 |
| language | English |
| last_indexed | 2024-04-12T03:23:27Z |
| publishDate | 2017-11-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-ee8b2738bb544d25831fca7ffa5e67e02022-12-22T03:49:48ZengBioscientificaEndocrine Connections2049-36142049-36142017-11-016875876510.1530/EC-17-0230Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitusBorros Arneth0Institute of Laboratory Medicine and Pathobiochemistry, Molecular Diagnostics, University Hospital of the Universities of Giessen and Marburg, UKGM, Justus Liebig University, Giessen, Giessen, GermanyBackground: The origin of autoimmune disease type 1 diabetes is still unknown. Aim: This study assessed the activation of CD4+ and CD8+ T-lymphocytes by human insulin and human glutamate decarboxylase (GAD) in patients with type 1 or type 2 diabetes mellitus (DM) and healthy volunteers. Materials and methods: The expression of CD69, a marker of T-lymphocyte activity, was determined in whole blood samples by flow cytometry after 12 h of incubation with or without insulin or GAD. The analysis included samples from 12 type 1 DM patients, 14 type 2 DM patients and 12 healthy volunteers. Results: Significant increases in the number of activated CD4+ and CD8+ T-lymphocytes following pre-incubation of whole blood samples with human insulin or GAD were observed in samples from patients with type 1 DM, whereas no activation of these cells was detected in samples from either type 2 DM patients or healthy subjects. Discussion: These results indicated that latent pre-activation of CD4+ and CD8+ T-lymphocytes in response to insulin or GAD epitopes occurred in type 1 DM patients. Conclusion: These findings suggest that pre-immunization against insulin and/or GAD might be associated with the development of type 1 DM. Alternatively, these results might reflect a non-specific, bystander autoimmune response.http://www.endocrineconnections.com/content/6/8/758.fulltype 1 diabetes mellitustype 2 diabetes mellitusCD4+ T-lymphocytesCD8+ T-lymphocytesautoimmunityautoantibodiesinsulinGAD |
| spellingShingle | Borros Arneth Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus Endocrine Connections type 1 diabetes mellitus type 2 diabetes mellitus CD4+ T-lymphocytes CD8+ T-lymphocytes autoimmunity autoantibodies insulin GAD |
| title | Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus |
| title_full | Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus |
| title_fullStr | Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus |
| title_full_unstemmed | Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus |
| title_short | Activation of CD4+ and CD8+ T-lymphocytes by insulin and GAD in patients with type 1 or 2 diabetes mellitus |
| title_sort | activation of cd4 and cd8 t lymphocytes by insulin and gad in patients with type 1 or 2 diabetes mellitus |
| topic | type 1 diabetes mellitus type 2 diabetes mellitus CD4+ T-lymphocytes CD8+ T-lymphocytes autoimmunity autoantibodies insulin GAD |
| url | http://www.endocrineconnections.com/content/6/8/758.full |
| work_keys_str_mv | AT borrosarneth activationofcd4andcd8tlymphocytesbyinsulinandgadinpatientswithtype1or2diabetesmellitus |