Molecular and anatomical characterization of parabrachial neurons and their axonal projections
The parabrachial nucleus (PBN) is a major hub that receives sensory information from both internal and external environments. Specific populations of PBN neurons are involved in behaviors including food and water intake, nociceptive responses, breathing regulation, as well as learning and responding...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/81868 |
| _version_ | 1811225939414089728 |
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| author | Jordan L Pauli Jane Y Chen Marcus L Basiri Sekun Park Matthew E Carter Elisenda Sanz G Stanley McKnight Garret D Stuber Richard D Palmiter |
| author_facet | Jordan L Pauli Jane Y Chen Marcus L Basiri Sekun Park Matthew E Carter Elisenda Sanz G Stanley McKnight Garret D Stuber Richard D Palmiter |
| author_sort | Jordan L Pauli |
| collection | DOAJ |
| description | The parabrachial nucleus (PBN) is a major hub that receives sensory information from both internal and external environments. Specific populations of PBN neurons are involved in behaviors including food and water intake, nociceptive responses, breathing regulation, as well as learning and responding appropriately to threatening stimuli. However, it is unclear how many PBN neuron populations exist and how different behaviors may be encoded by unique signaling molecules or receptors. Here we provide a repository of data on the molecular identity, spatial location, and projection patterns of dozens of PBN neuron subclusters. Using single-cell RNA sequencing, we identified 21 subclusters of neurons in the PBN and neighboring regions. Multiplexed in situ hybridization showed many of these subclusters are enriched within specific PBN subregions with scattered cells in several other regions. We also provide detailed visualization of the axonal projections from 21 Cre-driver lines of mice. These results are all publicly available for download and provide a foundation for further interrogation of PBN functions and connections. |
| first_indexed | 2024-04-12T09:16:36Z |
| format | Article |
| id | doaj.art-ee8ce862de90472d976a0c522ff83497 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:16:36Z |
| publishDate | 2022-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ee8ce862de90472d976a0c522ff834972022-12-22T03:38:50ZengeLife Sciences Publications LtdeLife2050-084X2022-11-011110.7554/eLife.81868Molecular and anatomical characterization of parabrachial neurons and their axonal projectionsJordan L Pauli0https://orcid.org/0000-0001-6276-3407Jane Y Chen1https://orcid.org/0000-0002-3986-8785Marcus L Basiri2https://orcid.org/0000-0002-4829-7187Sekun Park3Matthew E Carter4https://orcid.org/0000-0003-1802-090XElisenda Sanz5https://orcid.org/0000-0002-7932-8556G Stanley McKnight6Garret D Stuber7https://orcid.org/0000-0003-1730-4855Richard D Palmiter8https://orcid.org/0000-0001-6587-0582Department of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United StatesDepartment of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United StatesCenter for the Neurobiology of Addiction, Pain, and Emotion, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, United States; Department of Pharmacology, University of Washington, Seattle, United StatesDepartment of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United StatesDepartment of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United StatesDepartment of Pharmacology, University of Washington, Seattle, United StatesDepartment of Pharmacology, University of Washington, Seattle, United StatesCenter for the Neurobiology of Addiction, Pain, and Emotion, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, United States; Department of Pharmacology, University of Washington, Seattle, United StatesDepartment of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United StatesThe parabrachial nucleus (PBN) is a major hub that receives sensory information from both internal and external environments. Specific populations of PBN neurons are involved in behaviors including food and water intake, nociceptive responses, breathing regulation, as well as learning and responding appropriately to threatening stimuli. However, it is unclear how many PBN neuron populations exist and how different behaviors may be encoded by unique signaling molecules or receptors. Here we provide a repository of data on the molecular identity, spatial location, and projection patterns of dozens of PBN neuron subclusters. Using single-cell RNA sequencing, we identified 21 subclusters of neurons in the PBN and neighboring regions. Multiplexed in situ hybridization showed many of these subclusters are enriched within specific PBN subregions with scattered cells in several other regions. We also provide detailed visualization of the axonal projections from 21 Cre-driver lines of mice. These results are all publicly available for download and provide a foundation for further interrogation of PBN functions and connections.https://elifesciences.org/articles/81868parabrachial nucleusscRNA sequencingmultiplex in situ hybridization |
| spellingShingle | Jordan L Pauli Jane Y Chen Marcus L Basiri Sekun Park Matthew E Carter Elisenda Sanz G Stanley McKnight Garret D Stuber Richard D Palmiter Molecular and anatomical characterization of parabrachial neurons and their axonal projections eLife parabrachial nucleus scRNA sequencing multiplex in situ hybridization |
| title | Molecular and anatomical characterization of parabrachial neurons and their axonal projections |
| title_full | Molecular and anatomical characterization of parabrachial neurons and their axonal projections |
| title_fullStr | Molecular and anatomical characterization of parabrachial neurons and their axonal projections |
| title_full_unstemmed | Molecular and anatomical characterization of parabrachial neurons and their axonal projections |
| title_short | Molecular and anatomical characterization of parabrachial neurons and their axonal projections |
| title_sort | molecular and anatomical characterization of parabrachial neurons and their axonal projections |
| topic | parabrachial nucleus scRNA sequencing multiplex in situ hybridization |
| url | https://elifesciences.org/articles/81868 |
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