Gene Expression Profiling of Skeletal Muscles
Next-generation sequencing provides an opportunity for an in-depth biocomputational analysis to identify gene expression patterns between soleus and tibialis anterior, two well-characterized skeletal muscles, and analyze their gene expression profiling. RNA read counts were analyzed for differential...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/11/1718 |
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| author | Sarah I. Alto Chih-Ning Chang Kevin Brown Chrissa Kioussi Theresa M. Filtz |
| author_facet | Sarah I. Alto Chih-Ning Chang Kevin Brown Chrissa Kioussi Theresa M. Filtz |
| author_sort | Sarah I. Alto |
| collection | DOAJ |
| description | Next-generation sequencing provides an opportunity for an in-depth biocomputational analysis to identify gene expression patterns between soleus and tibialis anterior, two well-characterized skeletal muscles, and analyze their gene expression profiling. RNA read counts were analyzed for differential gene expression using the R package edgeR. Differentially expressed genes were filtered using a false discovery rate of less than 0.05 c, a fold-change value of more than twenty, and an association with overrepresented pathways based on the Reactome pathway over-representation analysis tool. Most of the differentially expressed genes associated with soleus are coded for components of lipid metabolism and unique contractile elements. Differentially expressed genes associated with tibialis anterior encoded mostly for glucose and glycogen metabolic pathway regulatory enzymes and calcium-sensitive contractile components. These gene expression distinctions partly explain the genetic basis for skeletal muscle specialization, and they may help to explain skeletal muscle susceptibility to disease and drugs and further refine tissue engineering approaches. |
| first_indexed | 2024-03-10T05:29:03Z |
| format | Article |
| id | doaj.art-ee8cfaba88f74fd19afa9fb273f99706 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T05:29:03Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-ee8cfaba88f74fd19afa9fb273f997062023-11-22T23:27:46ZengMDPI AGGenes2073-44252021-10-011211171810.3390/genes12111718Gene Expression Profiling of Skeletal MusclesSarah I. Alto0Chih-Ning Chang1Kevin Brown2Chrissa Kioussi3Theresa M. Filtz4Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USADepartment of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USADepartment of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USADepartment of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USADepartment of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USANext-generation sequencing provides an opportunity for an in-depth biocomputational analysis to identify gene expression patterns between soleus and tibialis anterior, two well-characterized skeletal muscles, and analyze their gene expression profiling. RNA read counts were analyzed for differential gene expression using the R package edgeR. Differentially expressed genes were filtered using a false discovery rate of less than 0.05 c, a fold-change value of more than twenty, and an association with overrepresented pathways based on the Reactome pathway over-representation analysis tool. Most of the differentially expressed genes associated with soleus are coded for components of lipid metabolism and unique contractile elements. Differentially expressed genes associated with tibialis anterior encoded mostly for glucose and glycogen metabolic pathway regulatory enzymes and calcium-sensitive contractile components. These gene expression distinctions partly explain the genetic basis for skeletal muscle specialization, and they may help to explain skeletal muscle susceptibility to disease and drugs and further refine tissue engineering approaches.https://www.mdpi.com/2073-4425/12/11/1718mouseskeletal musclesoleustibialis anteriorgene expressionmetabolism |
| spellingShingle | Sarah I. Alto Chih-Ning Chang Kevin Brown Chrissa Kioussi Theresa M. Filtz Gene Expression Profiling of Skeletal Muscles Genes mouse skeletal muscle soleus tibialis anterior gene expression metabolism |
| title | Gene Expression Profiling of Skeletal Muscles |
| title_full | Gene Expression Profiling of Skeletal Muscles |
| title_fullStr | Gene Expression Profiling of Skeletal Muscles |
| title_full_unstemmed | Gene Expression Profiling of Skeletal Muscles |
| title_short | Gene Expression Profiling of Skeletal Muscles |
| title_sort | gene expression profiling of skeletal muscles |
| topic | mouse skeletal muscle soleus tibialis anterior gene expression metabolism |
| url | https://www.mdpi.com/2073-4425/12/11/1718 |
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