In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii
Acinetobacter baumannii is a multidrug-resistant pathogen that represents a serious threat to global health. A. baumannii possesses a wide range of virulence factors that contribute to the bacterial pathogenicity. Among them, the siderophore acinetobactin is one of the most important, being essentia...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.752070/full |
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| author | Kelly Conde-Pérez Kelly Conde-Pérez Juan C. Vázquez-Ucha Laura Álvarez-Fraga Laura Álvarez-Fraga Lucía Ageitos Soraya Rumbo-Feal Soraya Rumbo-Feal Marta Martínez-Guitián Noelia Trigo-Tasende Jaime Rodríguez Germán Bou Carlos Jiménez Alejandro Beceiro Margarita Poza Margarita Poza |
| author_facet | Kelly Conde-Pérez Kelly Conde-Pérez Juan C. Vázquez-Ucha Laura Álvarez-Fraga Laura Álvarez-Fraga Lucía Ageitos Soraya Rumbo-Feal Soraya Rumbo-Feal Marta Martínez-Guitián Noelia Trigo-Tasende Jaime Rodríguez Germán Bou Carlos Jiménez Alejandro Beceiro Margarita Poza Margarita Poza |
| author_sort | Kelly Conde-Pérez |
| collection | DOAJ |
| description | Acinetobacter baumannii is a multidrug-resistant pathogen that represents a serious threat to global health. A. baumannii possesses a wide range of virulence factors that contribute to the bacterial pathogenicity. Among them, the siderophore acinetobactin is one of the most important, being essential for the development of the infection. In this study we performed an in-depth analysis of the acinetobactin cluster in the strain A. baumannii ATCC 17978. For this purpose, nineteen individual isogenic mutant strains were generated, and further phenotypical analysis were performed. Individual mutants lacking the biosynthetic genes entA, basG, basC, basD, and basB showed a significant loss in virulence, due to the disruption in the acinetobactin production. Similarly, the gene bauA, coding for the acinetobactin receptor, was also found to be crucial for the bacterial pathogenesis. In addition, the analysis of the ΔbasJ/ΔfbsB double mutant strain demonstrated the high level of genetic redundancy between siderophores where the role of specific genes of the acinetobactin cluster can be fulfilled by their fimsbactin redundant genes. Overall, this study highlights the essential role of entA, basG, basC, basD, basB and bauA in the pathogenicity of A. baumannii and provides potential therapeutic targets for the design of new antivirulence agents against this microorganism. |
| first_indexed | 2024-12-19T14:55:15Z |
| format | Article |
| id | doaj.art-ee8eaab4b4214e8caad8fec9e25b4839 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T14:55:15Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ee8eaab4b4214e8caad8fec9e25b48392022-12-21T20:16:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-10-011210.3389/fmicb.2021.752070752070In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumanniiKelly Conde-Pérez0Kelly Conde-Pérez1Juan C. Vázquez-Ucha2Laura Álvarez-Fraga3Laura Álvarez-Fraga4Lucía Ageitos5Soraya Rumbo-Feal6Soraya Rumbo-Feal7Marta Martínez-Guitián8Noelia Trigo-Tasende9Jaime Rodríguez10Germán Bou11Carlos Jiménez12Alejandro Beceiro13Margarita Poza14Margarita Poza15Servicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainMicrobiome and Health, Faculty of Science, University of A Coruña, A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainSchool of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, AustraliaCentro de Investigaciones Científicas Avanzadas (CICA) y Departamento de Química, Facultad de Ciencias, Agrupación Estratégica CICA-INIBIC, Universidad de A Coruña, A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainMicrobiome and Health, Faculty of Science, University of A Coruña, A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainCentro de Investigaciones Científicas Avanzadas (CICA) y Departamento de Química, Facultad de Ciencias, Agrupación Estratégica CICA-INIBIC, Universidad de A Coruña, A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainCentro de Investigaciones Científicas Avanzadas (CICA) y Departamento de Química, Facultad de Ciencias, Agrupación Estratégica CICA-INIBIC, Universidad de A Coruña, A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainServicio de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, SpainMicrobiome and Health, Faculty of Science, University of A Coruña, A Coruña, SpainAcinetobacter baumannii is a multidrug-resistant pathogen that represents a serious threat to global health. A. baumannii possesses a wide range of virulence factors that contribute to the bacterial pathogenicity. Among them, the siderophore acinetobactin is one of the most important, being essential for the development of the infection. In this study we performed an in-depth analysis of the acinetobactin cluster in the strain A. baumannii ATCC 17978. For this purpose, nineteen individual isogenic mutant strains were generated, and further phenotypical analysis were performed. Individual mutants lacking the biosynthetic genes entA, basG, basC, basD, and basB showed a significant loss in virulence, due to the disruption in the acinetobactin production. Similarly, the gene bauA, coding for the acinetobactin receptor, was also found to be crucial for the bacterial pathogenesis. In addition, the analysis of the ΔbasJ/ΔfbsB double mutant strain demonstrated the high level of genetic redundancy between siderophores where the role of specific genes of the acinetobactin cluster can be fulfilled by their fimsbactin redundant genes. Overall, this study highlights the essential role of entA, basG, basC, basD, basB and bauA in the pathogenicity of A. baumannii and provides potential therapeutic targets for the design of new antivirulence agents against this microorganism.https://www.frontiersin.org/articles/10.3389/fmicb.2021.752070/fullAcinetobacter baumanniiacinetobactinfimsbactiniron uptakesiderophorevirulence |
| spellingShingle | Kelly Conde-Pérez Kelly Conde-Pérez Juan C. Vázquez-Ucha Laura Álvarez-Fraga Laura Álvarez-Fraga Lucía Ageitos Soraya Rumbo-Feal Soraya Rumbo-Feal Marta Martínez-Guitián Noelia Trigo-Tasende Jaime Rodríguez Germán Bou Carlos Jiménez Alejandro Beceiro Margarita Poza Margarita Poza In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii Frontiers in Microbiology Acinetobacter baumannii acinetobactin fimsbactin iron uptake siderophore virulence |
| title | In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii |
| title_full | In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii |
| title_fullStr | In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii |
| title_full_unstemmed | In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii |
| title_short | In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii |
| title_sort | in depth analysis of the role of the acinetobactin cluster in the virulence of acinetobacter baumannii |
| topic | Acinetobacter baumannii acinetobactin fimsbactin iron uptake siderophore virulence |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.752070/full |
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