Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation
Gastrointestinal microbiota can be easily altered by common treatments, such as antibiotic therapy. However, the dysmicrobism induced by such a treatment may be counteracted by the administration of different beneficial microbes, such as probiotics. Therefore, this study aimed to establish the inter...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/5/1105 |
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| author | Maria-Cătălina Matei-Lațiu Adrian-Florin Gal Vasile Rus Victoria Buza Cristian Martonos Călin Lațiu Laura-Cristina Ștefănuț |
| author_facet | Maria-Cătălina Matei-Lațiu Adrian-Florin Gal Vasile Rus Victoria Buza Cristian Martonos Călin Lațiu Laura-Cristina Ștefănuț |
| author_sort | Maria-Cătălina Matei-Lațiu |
| collection | DOAJ |
| description | Gastrointestinal microbiota can be easily altered by common treatments, such as antibiotic therapy. However, the dysmicrobism induced by such a treatment may be counteracted by the administration of different beneficial microbes, such as probiotics. Therefore, this study aimed to establish the interaction between intestinal microbiota, antibiotic therapy, and sporulated bacteria, correlated with the evolution of growth indices. Twenty-five Wistar rats, females, were divided into five groups. Amoxicillin and a probiotic combination of <i>Bacillus subtilis</i>, <i>Bacillus licheniformis,</i> and <i>Pediococcus acidilactici</i> were administered according to each group’s purpose. The conventional growth indices were calculated and histological and immunohistochemical assessments were realized from intestinal samples. The results of the conventional growth indices suggested a beneficial effect when the antibiotic therapy was accompanied by probiotics, while for the groups where the dysmicrobism was present, the values for feed conversion ratio were negative. These findings were supported by the microscopic aspects of the intestinal mucosa, which suggested a decreased absorption capacity due to significant morphological changes. Moreover, the immunohistochemical reaction of the inflammatory cells from intestinal lamina propria was intensely positive for the same affected groups. However, for the control group and the group with antibiotic and probiotic treatment, the immunopositivity was significantly decreased. Probiotics based on <i>bacillus</i> spores administered simultaneously with the antibiotic offered the best restoration of the gut microbiota, a fact suggested by the absence of intestinal lesions, a normal food conversion ratio, and low expression of TLR4 and LBP immunomarkers. |
| first_indexed | 2024-03-11T07:15:04Z |
| format | Article |
| id | doaj.art-ee92bfcef7eb4ba7b27b310afa6927be |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T07:15:04Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ee92bfcef7eb4ba7b27b310afa6927be2023-11-17T08:19:41ZengMDPI AGNutrients2072-66432023-02-01155110510.3390/nu15051105Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical EvaluationMaria-Cătălina Matei-Lațiu0Adrian-Florin Gal1Vasile Rus2Victoria Buza3Cristian Martonos4Călin Lațiu5Laura-Cristina Ștefănuț6Department of Animal Physiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Histology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Histology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Anatomy, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, RomaniaFaculty of Animal Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, RomaniaGastrointestinal microbiota can be easily altered by common treatments, such as antibiotic therapy. However, the dysmicrobism induced by such a treatment may be counteracted by the administration of different beneficial microbes, such as probiotics. Therefore, this study aimed to establish the interaction between intestinal microbiota, antibiotic therapy, and sporulated bacteria, correlated with the evolution of growth indices. Twenty-five Wistar rats, females, were divided into five groups. Amoxicillin and a probiotic combination of <i>Bacillus subtilis</i>, <i>Bacillus licheniformis,</i> and <i>Pediococcus acidilactici</i> were administered according to each group’s purpose. The conventional growth indices were calculated and histological and immunohistochemical assessments were realized from intestinal samples. The results of the conventional growth indices suggested a beneficial effect when the antibiotic therapy was accompanied by probiotics, while for the groups where the dysmicrobism was present, the values for feed conversion ratio were negative. These findings were supported by the microscopic aspects of the intestinal mucosa, which suggested a decreased absorption capacity due to significant morphological changes. Moreover, the immunohistochemical reaction of the inflammatory cells from intestinal lamina propria was intensely positive for the same affected groups. However, for the control group and the group with antibiotic and probiotic treatment, the immunopositivity was significantly decreased. Probiotics based on <i>bacillus</i> spores administered simultaneously with the antibiotic offered the best restoration of the gut microbiota, a fact suggested by the absence of intestinal lesions, a normal food conversion ratio, and low expression of TLR4 and LBP immunomarkers.https://www.mdpi.com/2072-6643/15/5/1105dysmicrobismantibioticsTLR4LBP<i>Bacillus</i> spp. |
| spellingShingle | Maria-Cătălina Matei-Lațiu Adrian-Florin Gal Vasile Rus Victoria Buza Cristian Martonos Călin Lațiu Laura-Cristina Ștefănuț Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation Nutrients dysmicrobism antibiotics TLR4 LBP <i>Bacillus</i> spp. |
| title | Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation |
| title_full | Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation |
| title_fullStr | Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation |
| title_full_unstemmed | Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation |
| title_short | Intestinal Dysbiosis in Rats: Interaction between Amoxicillin and Probiotics, a Histological and Immunohistochemical Evaluation |
| title_sort | intestinal dysbiosis in rats interaction between amoxicillin and probiotics a histological and immunohistochemical evaluation |
| topic | dysmicrobism antibiotics TLR4 LBP <i>Bacillus</i> spp. |
| url | https://www.mdpi.com/2072-6643/15/5/1105 |
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