Developing Wolbachia-based disease interventions for an extreme environment.
Aedes aegypti mosquitoes carrying self-spreading, virus-blocking Wolbachia bacteria are being deployed to suppress dengue transmission. However, there are challenges in applying this technology in extreme environments. We introduced two Wolbachia strains into Ae. aegypti from Saudi Arabia for a rele...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1011117 |
| _version_ | 1797904253422927872 |
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| author | Perran A Ross Samia Elfekih Sophie Collier Melissa J Klein Su Shyan Lee Michael Dunn Sarah Jackson Yexin Zhang Jason K Axford Xinyue Gu Jessica L Home Majed S Nassar Prasad N Paradkar Essam A Tawfik Francis M Jiggins Abdulaziz M Almalik Mohamed B Al-Fageeh Ary A Hoffmann |
| author_facet | Perran A Ross Samia Elfekih Sophie Collier Melissa J Klein Su Shyan Lee Michael Dunn Sarah Jackson Yexin Zhang Jason K Axford Xinyue Gu Jessica L Home Majed S Nassar Prasad N Paradkar Essam A Tawfik Francis M Jiggins Abdulaziz M Almalik Mohamed B Al-Fageeh Ary A Hoffmann |
| author_sort | Perran A Ross |
| collection | DOAJ |
| description | Aedes aegypti mosquitoes carrying self-spreading, virus-blocking Wolbachia bacteria are being deployed to suppress dengue transmission. However, there are challenges in applying this technology in extreme environments. We introduced two Wolbachia strains into Ae. aegypti from Saudi Arabia for a release program in the hot coastal city of Jeddah. Wolbachia reduced infection and dissemination of dengue virus (DENV2) in Saudi Arabian mosquitoes and showed complete maternal transmission and cytoplasmic incompatibility. Wolbachia reduced egg hatch under a range of environmental conditions, with the Wolbachia strains showing differential thermal stability. Wolbachia effects were similar across mosquito genetic backgrounds but we found evidence of local adaptation, with Saudi Arabian mosquitoes having lower egg viability but higher adult desiccation tolerance than Australian mosquitoes. Genetic background effects will influence Wolbachia invasion dynamics, reinforcing the need to use local genotypes for mosquito release programs, particularly in extreme environments like Jeddah. Our comprehensive characterization of Wolbachia strains provides a foundation for Wolbachia-based disease interventions in harsh climates. |
| first_indexed | 2024-04-10T09:45:57Z |
| format | Article |
| id | doaj.art-ee96bdeb1b3e41a090721b41c0ac7b5a |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-10T09:45:57Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-ee96bdeb1b3e41a090721b41c0ac7b5a2023-02-17T05:31:45ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742023-01-01191e101111710.1371/journal.ppat.1011117Developing Wolbachia-based disease interventions for an extreme environment.Perran A RossSamia ElfekihSophie CollierMelissa J KleinSu Shyan LeeMichael DunnSarah JacksonYexin ZhangJason K AxfordXinyue GuJessica L HomeMajed S NassarPrasad N ParadkarEssam A TawfikFrancis M JigginsAbdulaziz M AlmalikMohamed B Al-FageehAry A HoffmannAedes aegypti mosquitoes carrying self-spreading, virus-blocking Wolbachia bacteria are being deployed to suppress dengue transmission. However, there are challenges in applying this technology in extreme environments. We introduced two Wolbachia strains into Ae. aegypti from Saudi Arabia for a release program in the hot coastal city of Jeddah. Wolbachia reduced infection and dissemination of dengue virus (DENV2) in Saudi Arabian mosquitoes and showed complete maternal transmission and cytoplasmic incompatibility. Wolbachia reduced egg hatch under a range of environmental conditions, with the Wolbachia strains showing differential thermal stability. Wolbachia effects were similar across mosquito genetic backgrounds but we found evidence of local adaptation, with Saudi Arabian mosquitoes having lower egg viability but higher adult desiccation tolerance than Australian mosquitoes. Genetic background effects will influence Wolbachia invasion dynamics, reinforcing the need to use local genotypes for mosquito release programs, particularly in extreme environments like Jeddah. Our comprehensive characterization of Wolbachia strains provides a foundation for Wolbachia-based disease interventions in harsh climates.https://doi.org/10.1371/journal.ppat.1011117 |
| spellingShingle | Perran A Ross Samia Elfekih Sophie Collier Melissa J Klein Su Shyan Lee Michael Dunn Sarah Jackson Yexin Zhang Jason K Axford Xinyue Gu Jessica L Home Majed S Nassar Prasad N Paradkar Essam A Tawfik Francis M Jiggins Abdulaziz M Almalik Mohamed B Al-Fageeh Ary A Hoffmann Developing Wolbachia-based disease interventions for an extreme environment. PLoS Pathogens |
| title | Developing Wolbachia-based disease interventions for an extreme environment. |
| title_full | Developing Wolbachia-based disease interventions for an extreme environment. |
| title_fullStr | Developing Wolbachia-based disease interventions for an extreme environment. |
| title_full_unstemmed | Developing Wolbachia-based disease interventions for an extreme environment. |
| title_short | Developing Wolbachia-based disease interventions for an extreme environment. |
| title_sort | developing wolbachia based disease interventions for an extreme environment |
| url | https://doi.org/10.1371/journal.ppat.1011117 |
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