The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats
The aim of this study was to investigate the role of inducible nitric oxide synthase (iNOS) in gentamicin-induced acute tubular necrosis in rats using the iNOS inhibitor L-N6-(1-iminoethyl) lysine (L-NIL). Wistar rats, both sexes (n=18), were equally divided into three groups. Gentamicin group rece...
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| Format: | Article |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2007-11-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/3025 |
| _version_ | 1797260855826448384 |
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| author | Nesina Avdagić Emina Nakaš-Ićindić Senija Rašić Almira Hadžović-Džuvo Asija Začiragić Amina Valjevac |
| author_facet | Nesina Avdagić Emina Nakaš-Ićindić Senija Rašić Almira Hadžović-Džuvo Asija Začiragić Amina Valjevac |
| author_sort | Nesina Avdagić |
| collection | DOAJ |
| description |
The aim of this study was to investigate the role of inducible nitric oxide synthase (iNOS) in gentamicin-induced acute tubular necrosis in rats using the iNOS inhibitor L-N6-(1-iminoethyl) lysine (L-NIL). Wistar rats, both sexes (n=18), were equally divided into three groups. Gentamicin group received intraperitoneally (i.p.) gentamicin in 0,9 % NaCl at a dose of 80 mg/kg/day for five consecutive days. L-NIL+gentamicin group received L-NIL at a dose of 3mg/kg i.p. 36, 24 and 12 h before first dose of gentamicin. Control group received 0,9 % NaCl i.p. for five consecutive days at the equal volume as gentamicin group. Griess reaction was used for determination plasma level of NO. Semiquantitative histological analysis was used for the evaluation of kidney damage level. The plasma NO level and the level of kidney damage were statistically higher in gentamicin group in comparison to the control group (p=0,046). Application of L-NIL prior to gentamicin led to certain decrease in the plasma level of NO as well as in the level of kidney damage. Application of L-NIL, prior to gentamicin administration, did not provide complete protective effects of L-NIL on the kidney, which was demonstrated on kidney sections. The lack of anticipated protective effect of L-NIL on kidney tissue might be explained with the fact that we have used L-NIL prior but not during/after gentamicin administration. It would be necessary to examine the effects of L-NIL administration not only before, but as well during and possibly after the administration of gentamicin.
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| first_indexed | 2024-04-24T23:31:58Z |
| format | Article |
| id | doaj.art-ee96f674d8a44170bff4e10f638af40e |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:31:58Z |
| publishDate | 2007-11-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-ee96f674d8a44170bff4e10f638af40e2024-03-15T14:38:17ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2007-11-017410.17305/bjbms.2007.3025610The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in RatsNesina Avdagić0Emina Nakaš-Ićindić1Senija Rašić2Almira Hadžović-Džuvo3Asija Začiragić4Amina Valjevac5Institute of Physiology and Biochemistry, Faculty ofMedicine, University of SarajevoInstitute of Physiology and Biochemistry, Faculty ofMedicine, University of SarajevoInstitute of Nephrology, University Clinical Centre SarajevoInstitute of Physiology and Biochemistry, Faculty ofMedicine, University of SarajevoInstitute of Physiology and Biochemistry, Faculty ofMedicine, University of SarajevoInstitute of Physiology and Biochemistry, Faculty ofMedicine, University of Sarajevo The aim of this study was to investigate the role of inducible nitric oxide synthase (iNOS) in gentamicin-induced acute tubular necrosis in rats using the iNOS inhibitor L-N6-(1-iminoethyl) lysine (L-NIL). Wistar rats, both sexes (n=18), were equally divided into three groups. Gentamicin group received intraperitoneally (i.p.) gentamicin in 0,9 % NaCl at a dose of 80 mg/kg/day for five consecutive days. L-NIL+gentamicin group received L-NIL at a dose of 3mg/kg i.p. 36, 24 and 12 h before first dose of gentamicin. Control group received 0,9 % NaCl i.p. for five consecutive days at the equal volume as gentamicin group. Griess reaction was used for determination plasma level of NO. Semiquantitative histological analysis was used for the evaluation of kidney damage level. The plasma NO level and the level of kidney damage were statistically higher in gentamicin group in comparison to the control group (p=0,046). Application of L-NIL prior to gentamicin led to certain decrease in the plasma level of NO as well as in the level of kidney damage. Application of L-NIL, prior to gentamicin administration, did not provide complete protective effects of L-NIL on the kidney, which was demonstrated on kidney sections. The lack of anticipated protective effect of L-NIL on kidney tissue might be explained with the fact that we have used L-NIL prior but not during/after gentamicin administration. It would be necessary to examine the effects of L-NIL administration not only before, but as well during and possibly after the administration of gentamicin. https://www.bjbms.org/ojs/index.php/bjbms/article/view/3025nitric oxideinducible nitric oxide synthasegentamicinacute tubular necrosis |
| spellingShingle | Nesina Avdagić Emina Nakaš-Ićindić Senija Rašić Almira Hadžović-Džuvo Asija Začiragić Amina Valjevac The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats Biomolecules & Biomedicine nitric oxide inducible nitric oxide synthase gentamicin acute tubular necrosis |
| title | The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats |
| title_full | The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats |
| title_fullStr | The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats |
| title_full_unstemmed | The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats |
| title_short | The Effects of Inducible Nitric Oxide Synthase Inhibitor L-N6-(1-Iminoethyl) Lysine in Gentamicin-Induced Acute Tubular Necrosis in Rats |
| title_sort | effects of inducible nitric oxide synthase inhibitor l n6 1 iminoethyl lysine in gentamicin induced acute tubular necrosis in rats |
| topic | nitric oxide inducible nitric oxide synthase gentamicin acute tubular necrosis |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/3025 |
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