Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal Microbiome
Breastfeeding and introduction of solid food are the two major components of infant feeding practices that influence gut microbiota composition in early infancy. However, it is unclear whether additional factors influence the microbiota of infants either exclusively breastfed or not breastfed. We ob...
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MDPI AG
2022-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/1/202 |
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| author | Tengfei Ma Sihan Bu Nigel Paneth Jean M. Kerver Sarah S. Comstock |
| author_facet | Tengfei Ma Sihan Bu Nigel Paneth Jean M. Kerver Sarah S. Comstock |
| author_sort | Tengfei Ma |
| collection | DOAJ |
| description | Breastfeeding and introduction of solid food are the two major components of infant feeding practices that influence gut microbiota composition in early infancy. However, it is unclear whether additional factors influence the microbiota of infants either exclusively breastfed or not breastfed. We obtained 194 fecal samples from infants at 3–9 months of age, extracted DNA, and sequenced the V4 region of the 16S rRNA gene. Feeding practices and clinical information were collected by questionnaire and abstraction of birth certificates. The gut microbiota of infants who were exclusively breastfed displayed significantly lower Shannon diversity (<i>p</i>-adjust < 0.001) and different gut microbiota composition compared to infants who were not breastfed (<i>p</i>-value = 0.001). Among the exclusively breastfed infants, recipients of vitamin D supplements displayed significantly lower Shannon diversity (<i>p</i>-adjust = 0.007), and different gut microbiota composition structure than non-supplemented, breastfed infants (<i>p</i>-value = 0.02). MaAslin analysis identified microbial taxa that associated with breastfeeding and vitamin D supplementation. Breastfeeding and infant vitamin D supplement intake play an important role in shaping infant gut microbiota. |
| first_indexed | 2024-03-10T03:27:30Z |
| format | Article |
| id | doaj.art-ee9debccdcab4e1a8e7593579a478443 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:27:30Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ee9debccdcab4e1a8e7593579a4784432023-11-23T12:05:46ZengMDPI AGNutrients2072-66432022-01-0114120210.3390/nu14010202Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal MicrobiomeTengfei Ma0Sihan Bu1Nigel Paneth2Jean M. Kerver3Sarah S. Comstock4Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USADepartment of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USADepartment of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USADepartment of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USADepartment of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USABreastfeeding and introduction of solid food are the two major components of infant feeding practices that influence gut microbiota composition in early infancy. However, it is unclear whether additional factors influence the microbiota of infants either exclusively breastfed or not breastfed. We obtained 194 fecal samples from infants at 3–9 months of age, extracted DNA, and sequenced the V4 region of the 16S rRNA gene. Feeding practices and clinical information were collected by questionnaire and abstraction of birth certificates. The gut microbiota of infants who were exclusively breastfed displayed significantly lower Shannon diversity (<i>p</i>-adjust < 0.001) and different gut microbiota composition compared to infants who were not breastfed (<i>p</i>-value = 0.001). Among the exclusively breastfed infants, recipients of vitamin D supplements displayed significantly lower Shannon diversity (<i>p</i>-adjust = 0.007), and different gut microbiota composition structure than non-supplemented, breastfed infants (<i>p</i>-value = 0.02). MaAslin analysis identified microbial taxa that associated with breastfeeding and vitamin D supplementation. Breastfeeding and infant vitamin D supplement intake play an important role in shaping infant gut microbiota.https://www.mdpi.com/2072-6643/14/1/202infant gut microbiotafeeding practicesdietbreastfeedingvitamin D supplementationcohort |
| spellingShingle | Tengfei Ma Sihan Bu Nigel Paneth Jean M. Kerver Sarah S. Comstock Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal Microbiome Nutrients infant gut microbiota feeding practices diet breastfeeding vitamin D supplementation cohort |
| title | Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal Microbiome |
| title_full | Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal Microbiome |
| title_fullStr | Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal Microbiome |
| title_full_unstemmed | Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal Microbiome |
| title_short | Vitamin D Supplementation in Exclusively Breastfed Infants Is Associated with Alterations in the Fecal Microbiome |
| title_sort | vitamin d supplementation in exclusively breastfed infants is associated with alterations in the fecal microbiome |
| topic | infant gut microbiota feeding practices diet breastfeeding vitamin D supplementation cohort |
| url | https://www.mdpi.com/2072-6643/14/1/202 |
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