Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale
Nanomechanics of cytoskeleton is deeply involved in physiology and regulation of cell behavior. Atomic Force Microscopy has been extensively used for quantitative characterization with high-spatial resolution, in particular showing tremendous opportunities in biomechanics by quantifying mechanical p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Advances in Physics: X |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23746149.2020.1866668 |
| _version_ | 1818576792772935680 |
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| author | Guoqiao Zhou Bokai Zhang Guanlin Tang Xue-Feng Yu Massimiliano Galluzzi |
| author_facet | Guoqiao Zhou Bokai Zhang Guanlin Tang Xue-Feng Yu Massimiliano Galluzzi |
| author_sort | Guoqiao Zhou |
| collection | DOAJ |
| description | Nanomechanics of cytoskeleton is deeply involved in physiology and regulation of cell behavior. Atomic Force Microscopy has been extensively used for quantitative characterization with high-spatial resolution, in particular showing tremendous opportunities in biomechanics by quantifying mechanical parameters related to cytoskeleton organization. In this short review, we highlight recent developments in cell nanomechanics by AFM focusing on methodology and direct application to investigate cytoskeleton restructuration when cells are interacting with nanostructures (surfaces and nanoparticles). In particular, cells can sense the stiffness of environment or internalized particles and AFM can detect the rearrangement of cytoskeleton as one of the responses of mechanotransduction stimuli. Current bottlenecks hindering further progress in technology, such as theoretical models of interpretation will be discussed, in particular we propose a solution for complex system by coupling AFM with finite element simulations to retrieve more quantitative information when heterogeneity and convolution play important roles. Finally, we present recent cutting-edge research directions to explore new techniques and enhance the capabilities of AFM nanomechanics for living cells. |
| first_indexed | 2024-12-16T06:19:39Z |
| format | Article |
| id | doaj.art-eea0dd0fe12743a9bdd7e5a5fd84884c |
| institution | Directory Open Access Journal |
| issn | 2374-6149 |
| language | English |
| last_indexed | 2024-12-16T06:19:39Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Advances in Physics: X |
| spelling | doaj.art-eea0dd0fe12743a9bdd7e5a5fd84884c2022-12-21T22:41:10ZengTaylor & Francis GroupAdvances in Physics: X2374-61492021-01-016110.1080/23746149.2020.18666681866668Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscaleGuoqiao Zhou0Bokai Zhang1Guanlin Tang2Xue-Feng Yu3Massimiliano Galluzzi4Materials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesBenHealth Biopharmaceutical Co., LTDUniversity of New MexicoMaterials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesMaterials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesNanomechanics of cytoskeleton is deeply involved in physiology and regulation of cell behavior. Atomic Force Microscopy has been extensively used for quantitative characterization with high-spatial resolution, in particular showing tremendous opportunities in biomechanics by quantifying mechanical parameters related to cytoskeleton organization. In this short review, we highlight recent developments in cell nanomechanics by AFM focusing on methodology and direct application to investigate cytoskeleton restructuration when cells are interacting with nanostructures (surfaces and nanoparticles). In particular, cells can sense the stiffness of environment or internalized particles and AFM can detect the rearrangement of cytoskeleton as one of the responses of mechanotransduction stimuli. Current bottlenecks hindering further progress in technology, such as theoretical models of interpretation will be discussed, in particular we propose a solution for complex system by coupling AFM with finite element simulations to retrieve more quantitative information when heterogeneity and convolution play important roles. Finally, we present recent cutting-edge research directions to explore new techniques and enhance the capabilities of AFM nanomechanics for living cells.http://dx.doi.org/10.1080/23746149.2020.1866668atomic force microscopy (afm)living cellsnanomechanicsnanoscale interactionsfinite element modeling |
| spellingShingle | Guoqiao Zhou Bokai Zhang Guanlin Tang Xue-Feng Yu Massimiliano Galluzzi Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale Advances in Physics: X atomic force microscopy (afm) living cells nanomechanics nanoscale interactions finite element modeling |
| title | Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale |
| title_full | Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale |
| title_fullStr | Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale |
| title_full_unstemmed | Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale |
| title_short | Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale |
| title_sort | cells nanomechanics by atomic force microscopy focus on interactions at nanoscale |
| topic | atomic force microscopy (afm) living cells nanomechanics nanoscale interactions finite element modeling |
| url | http://dx.doi.org/10.1080/23746149.2020.1866668 |
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