Important amino acid residues of potato plant uncoupling protein (StUCP)
Chemical modifications were used to identify some of the functionally important amino acid residues of the potato plant uncoupling protein (StUCP). The proton-dependent swelling of potato mitochondria in K+-acetate in the presence of linoleic acid and valinomycin was inhibited by mersalyl (Ki = 5 µM...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2000-12-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200003 |
| _version_ | 1818155695597420544 |
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| author | P. Jezek A.D.T. Costa A.E. Vercesi |
| author_facet | P. Jezek A.D.T. Costa A.E. Vercesi |
| author_sort | P. Jezek |
| collection | DOAJ |
| description | Chemical modifications were used to identify some of the functionally important amino acid residues of the potato plant uncoupling protein (StUCP). The proton-dependent swelling of potato mitochondria in K+-acetate in the presence of linoleic acid and valinomycin was inhibited by mersalyl (Ki = 5 µM) and other hydrophilic SH reagents such as Thiolyte MB, iodoacetate and 5,5'-dithio-bis-(2-nitrobenzoate), but not by hydrophobic N-ethylmaleimide. This pattern of inhibition by SH reagents was similar to that of brown adipose tissue uncoupling protein (UCP1). As with UCP1, the arginine reagent 2,3-butadione, but not N-ethylmaleimide or other hydrophobic SH reagents, prevented the inhibition of StUCP-mediated transport by ATP in isolated potato mitochondria or with reconstituted StUCP. The results indicate that the most reactive amino acid residues in UCP1 and StUCP are similar, with the exception of N-ethylmaleimide-reactive cysteines in the purine nucleotide-binding site. |
| first_indexed | 2024-12-11T14:46:30Z |
| format | Article |
| id | doaj.art-eea180cb63574416a6fa02239c15ba8f |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-11T14:46:30Z |
| publishDate | 2000-12-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-eea180cb63574416a6fa02239c15ba8f2022-12-22T01:01:41ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2000-12-0133121413142010.1590/S0100-879X2000001200003Important amino acid residues of potato plant uncoupling protein (StUCP)P. JezekA.D.T. CostaA.E. VercesiChemical modifications were used to identify some of the functionally important amino acid residues of the potato plant uncoupling protein (StUCP). The proton-dependent swelling of potato mitochondria in K+-acetate in the presence of linoleic acid and valinomycin was inhibited by mersalyl (Ki = 5 µM) and other hydrophilic SH reagents such as Thiolyte MB, iodoacetate and 5,5'-dithio-bis-(2-nitrobenzoate), but not by hydrophobic N-ethylmaleimide. This pattern of inhibition by SH reagents was similar to that of brown adipose tissue uncoupling protein (UCP1). As with UCP1, the arginine reagent 2,3-butadione, but not N-ethylmaleimide or other hydrophobic SH reagents, prevented the inhibition of StUCP-mediated transport by ATP in isolated potato mitochondria or with reconstituted StUCP. The results indicate that the most reactive amino acid residues in UCP1 and StUCP are similar, with the exception of N-ethylmaleimide-reactive cysteines in the purine nucleotide-binding site.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200003plant mitochondriauncoupling proteinchemical modificationmitochondrial swellingreconstitution |
| spellingShingle | P. Jezek A.D.T. Costa A.E. Vercesi Important amino acid residues of potato plant uncoupling protein (StUCP) Brazilian Journal of Medical and Biological Research plant mitochondria uncoupling protein chemical modification mitochondrial swelling reconstitution |
| title | Important amino acid residues of potato plant uncoupling protein (StUCP) |
| title_full | Important amino acid residues of potato plant uncoupling protein (StUCP) |
| title_fullStr | Important amino acid residues of potato plant uncoupling protein (StUCP) |
| title_full_unstemmed | Important amino acid residues of potato plant uncoupling protein (StUCP) |
| title_short | Important amino acid residues of potato plant uncoupling protein (StUCP) |
| title_sort | important amino acid residues of potato plant uncoupling protein stucp |
| topic | plant mitochondria uncoupling protein chemical modification mitochondrial swelling reconstitution |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200003 |
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