Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides
<i>Ophiopogon japonicus</i> polysaccharides (OJPs) have great anti-inflammation and immunomodulatory abilities. However, the low bioavailability of OJPs reduces its applicability in the biomedical and pharmaceutical fields. Chitosan (CS) has excellent mucoadhesive properties and absorpti...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/14/2966 |
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| author | Chi Lin Fang-Yu Hsu Wei-Ting Lin Chia-Yun Cha Yi-Cheng Ho Fwu-Long Mi |
| author_facet | Chi Lin Fang-Yu Hsu Wei-Ting Lin Chia-Yun Cha Yi-Cheng Ho Fwu-Long Mi |
| author_sort | Chi Lin |
| collection | DOAJ |
| description | <i>Ophiopogon japonicus</i> polysaccharides (OJPs) have great anti-inflammation and immunomodulatory abilities. However, the low bioavailability of OJPs reduces its applicability in the biomedical and pharmaceutical fields. Chitosan (CS) has excellent mucoadhesive properties and absorption-enhancing ability in oral administration. Casein hydrolysate (CL) has good interfacial diffusivity and emulsifying ability, and can interact with polysaccharides to form complexes combining the individual properties of both. Therefore, chitosan and casein hydrolysate are good candidates for developing nanoformulations for oral delivery. In this study, bioactive polysaccharides (OJPs), CS and CL, were combined to prepare CS/OJPs/CL co-assembled biodegradable nanoparticles. The interactions between polysaccharides (CS and OJPs) and peptide (CL) resulted in the formation of nanoparticles with an average particle size of 198 nm and high OJPs loading efficiency. The colloidal properties of the nanoparticles were pH-dependent, which were changed significantly in simulated digestive fluid at different pH values. OJPs released from the CS/OJPs/CL nanoparticles were greatly affected by pH and enzymatic degradation (trypsin and lysozyme). The nanoparticles were easily internalized by macrophages, thereby enhancing the OJPs’ inhibitory ability against Ni<sup>2+</sup>-induced cytotoxicity and LPS-induced nitric oxide production. This study demonstrates that prepared polysaccharide/protein co-assembled nanoparticles can be potential nanocarriers for the oral delivery of bioactive polysaccharides with anti-inflammatory functions. |
| first_indexed | 2024-03-09T13:08:10Z |
| format | Article |
| id | doaj.art-eea22890c0af41f489f50cbc9095c2e6 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T13:08:10Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-eea22890c0af41f489f50cbc9095c2e62023-11-30T21:45:47ZengMDPI AGPolymers2073-43602022-07-011414296610.3390/polym14142966Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive PolysaccharidesChi Lin0Fang-Yu Hsu1Wei-Ting Lin2Chia-Yun Cha3Yi-Cheng Ho4Fwu-Long Mi5Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanDepartment of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanDepartment Oral and Maxillofacial Surgery, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi 60004, TaiwanDepartment of Bioagricultural Sciences, National Chiayi University, Chiayi 60004, TaiwanDepartment of Bioagricultural Sciences, National Chiayi University, Chiayi 60004, TaiwanGraduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan<i>Ophiopogon japonicus</i> polysaccharides (OJPs) have great anti-inflammation and immunomodulatory abilities. However, the low bioavailability of OJPs reduces its applicability in the biomedical and pharmaceutical fields. Chitosan (CS) has excellent mucoadhesive properties and absorption-enhancing ability in oral administration. Casein hydrolysate (CL) has good interfacial diffusivity and emulsifying ability, and can interact with polysaccharides to form complexes combining the individual properties of both. Therefore, chitosan and casein hydrolysate are good candidates for developing nanoformulations for oral delivery. In this study, bioactive polysaccharides (OJPs), CS and CL, were combined to prepare CS/OJPs/CL co-assembled biodegradable nanoparticles. The interactions between polysaccharides (CS and OJPs) and peptide (CL) resulted in the formation of nanoparticles with an average particle size of 198 nm and high OJPs loading efficiency. The colloidal properties of the nanoparticles were pH-dependent, which were changed significantly in simulated digestive fluid at different pH values. OJPs released from the CS/OJPs/CL nanoparticles were greatly affected by pH and enzymatic degradation (trypsin and lysozyme). The nanoparticles were easily internalized by macrophages, thereby enhancing the OJPs’ inhibitory ability against Ni<sup>2+</sup>-induced cytotoxicity and LPS-induced nitric oxide production. This study demonstrates that prepared polysaccharide/protein co-assembled nanoparticles can be potential nanocarriers for the oral delivery of bioactive polysaccharides with anti-inflammatory functions.https://www.mdpi.com/2073-4360/14/14/2966chitosanpolysaccharidesbioactive and biodegradablenanoparticlespH-responsiveoral delivery |
| spellingShingle | Chi Lin Fang-Yu Hsu Wei-Ting Lin Chia-Yun Cha Yi-Cheng Ho Fwu-Long Mi Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides Polymers chitosan polysaccharides bioactive and biodegradable nanoparticles pH-responsive oral delivery |
| title | Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides |
| title_full | Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides |
| title_fullStr | Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides |
| title_full_unstemmed | Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides |
| title_short | Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides |
| title_sort | biodegradable nanoparticles prepared from chitosan and casein for delivery of bioactive polysaccharides |
| topic | chitosan polysaccharides bioactive and biodegradable nanoparticles pH-responsive oral delivery |
| url | https://www.mdpi.com/2073-4360/14/14/2966 |
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