Natural antisense transcripts regulate the neuronal stress response and excitability
Neurons regulate ionic fluxes across their plasma membrane to maintain their excitable properties under varying environmental conditions. However, the mechanisms that regulate ion channels abundance remain poorly understood. Here we show that pickpocket 29 (ppk29), a gene that encodes a Drosophila d...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2014-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01849 |
| _version_ | 1811253337812631552 |
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| author | Xingguo Zheng Vera Valakh Aaron DiAntonio Yehuda Ben-Shahar |
| author_facet | Xingguo Zheng Vera Valakh Aaron DiAntonio Yehuda Ben-Shahar |
| author_sort | Xingguo Zheng |
| collection | DOAJ |
| description | Neurons regulate ionic fluxes across their plasma membrane to maintain their excitable properties under varying environmental conditions. However, the mechanisms that regulate ion channels abundance remain poorly understood. Here we show that pickpocket 29 (ppk29), a gene that encodes a Drosophila degenerin/epithelial sodium channel (DEG/ENaC), regulates neuronal excitability via a protein-independent mechanism. We demonstrate that the mRNA 3′UTR of ppk29 affects neuronal firing rates and associated heat-induced seizures by acting as a natural antisense transcript (NAT) that regulates the neuronal mRNA levels of seizure (sei), the Drosophila homolog of the human Ether-à-go-go Related Gene (hERG) potassium channel. We find that the regulatory impact of ppk29 mRNA on sei is independent of the sodium channel it encodes. Thus, our studies reveal a novel mRNA dependent mechanism for the regulation of neuronal excitability that is independent of protein-coding capacity. |
| first_indexed | 2024-04-12T16:49:22Z |
| format | Article |
| id | doaj.art-eea61016b00f4b49a52771217eb4fa28 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:49:22Z |
| publishDate | 2014-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-eea61016b00f4b49a52771217eb4fa282022-12-22T03:24:27ZengeLife Sciences Publications LtdeLife2050-084X2014-03-01310.7554/eLife.01849Natural antisense transcripts regulate the neuronal stress response and excitabilityXingguo Zheng0Vera Valakh1Aaron DiAntonio2Yehuda Ben-Shahar3Department of Biology, Washington University in St. Louis, St. Louis, United StatesDepartment of Developmental Biology, Washington University School of Medicine, St. Louis, United StatesDepartment of Developmental Biology, Washington University School of Medicine, St. Louis, United States; Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, United StatesDepartment of Biology, Washington University in St. Louis, St. Louis, United StatesNeurons regulate ionic fluxes across their plasma membrane to maintain their excitable properties under varying environmental conditions. However, the mechanisms that regulate ion channels abundance remain poorly understood. Here we show that pickpocket 29 (ppk29), a gene that encodes a Drosophila degenerin/epithelial sodium channel (DEG/ENaC), regulates neuronal excitability via a protein-independent mechanism. We demonstrate that the mRNA 3′UTR of ppk29 affects neuronal firing rates and associated heat-induced seizures by acting as a natural antisense transcript (NAT) that regulates the neuronal mRNA levels of seizure (sei), the Drosophila homolog of the human Ether-à-go-go Related Gene (hERG) potassium channel. We find that the regulatory impact of ppk29 mRNA on sei is independent of the sodium channel it encodes. Thus, our studies reveal a novel mRNA dependent mechanism for the regulation of neuronal excitability that is independent of protein-coding capacity.https://elifesciences.org/articles/01849DegenerinEpithelial sodium channelDEG/ENaCDrosophilafruit fly |
| spellingShingle | Xingguo Zheng Vera Valakh Aaron DiAntonio Yehuda Ben-Shahar Natural antisense transcripts regulate the neuronal stress response and excitability eLife Degenerin Epithelial sodium channel DEG/ENaC Drosophila fruit fly |
| title | Natural antisense transcripts regulate the neuronal stress response and excitability |
| title_full | Natural antisense transcripts regulate the neuronal stress response and excitability |
| title_fullStr | Natural antisense transcripts regulate the neuronal stress response and excitability |
| title_full_unstemmed | Natural antisense transcripts regulate the neuronal stress response and excitability |
| title_short | Natural antisense transcripts regulate the neuronal stress response and excitability |
| title_sort | natural antisense transcripts regulate the neuronal stress response and excitability |
| topic | Degenerin Epithelial sodium channel DEG/ENaC Drosophila fruit fly |
| url | https://elifesciences.org/articles/01849 |
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