Spectrophotometric determination of morin in strawberries and their antioxidant activity

Morin is one of the flavonoids with intensive antioxidant activity. With the aim to use its benefits on human health, there is an increasing trend to pay attention to its content in food or supplements. The simplicity and low cost of spectrophotometric determination based on the formation of a morin...

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Main Authors: Pavun Leposava, Janošević-Ležaić Aleksandra, Uskoković-Marković Snežana
Format: Article
Language:srp
Published: Pharmaceutical Association of Serbia, Belgrade, Serbia 2021-01-01
Series:Arhiv za farmaciju
Subjects:
Online Access:https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632101055P.pdf
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author Pavun Leposava
Janošević-Ležaić Aleksandra
Uskoković-Marković Snežana
author_facet Pavun Leposava
Janošević-Ležaić Aleksandra
Uskoković-Marković Snežana
author_sort Pavun Leposava
collection DOAJ
description Morin is one of the flavonoids with intensive antioxidant activity. With the aim to use its benefits on human health, there is an increasing trend to pay attention to its content in food or supplements. The simplicity and low cost of spectrophotometric determination based on the formation of a morin complex with Zn 2+ ion (stoichiometric ratio 1 : 1), at pH 7.98 and 392 nm, give it an advantage over other methods that can be used for morin quantification. The concentration range over which the response was linear was 0.151-4.533 mg L-1. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.030 mg L-1 and 0.091 mg L-1 , respectively. The developed method was successfully applied for the determination of the morin content in strawberries. Additionally, the antioxidative abilities of strawberry extracts and morin, determined by DPPH and FRAP tests, were compared and discussed.
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spelling doaj.art-eea9e77731a64be18c408adc3d41b94f2022-12-21T22:41:39ZsrpPharmaceutical Association of Serbia, Belgrade, SerbiaArhiv za farmaciju0004-19632217-87672021-01-01711557110.5937/arhfarm71-305030004-19632101055PSpectrophotometric determination of morin in strawberries and their antioxidant activityPavun Leposava0Janošević-Ležaić Aleksandra1https://orcid.org/0000-0003-4343-0572Uskoković-Marković Snežana2https://orcid.org/0000-0003-2750-325XUniversity of Belgrade, Faculty of Pharmacy, Department of Physical Chemistry and Instrumental Methods, Belgrade, SerbiaUniversity of Belgrade, Faculty of Pharmacy, Department of Physical Chemistry and Instrumental Methods, Belgrade, SerbiaUniversity of Belgrade, Faculty of Pharmacy, Department of Analytical Chemistry, Belgrade, SerbiaMorin is one of the flavonoids with intensive antioxidant activity. With the aim to use its benefits on human health, there is an increasing trend to pay attention to its content in food or supplements. The simplicity and low cost of spectrophotometric determination based on the formation of a morin complex with Zn 2+ ion (stoichiometric ratio 1 : 1), at pH 7.98 and 392 nm, give it an advantage over other methods that can be used for morin quantification. The concentration range over which the response was linear was 0.151-4.533 mg L-1. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.030 mg L-1 and 0.091 mg L-1 , respectively. The developed method was successfully applied for the determination of the morin content in strawberries. Additionally, the antioxidative abilities of strawberry extracts and morin, determined by DPPH and FRAP tests, were compared and discussed.https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632101055P.pdfmorinstrawberriesspectrophotometryzinc complex
spellingShingle Pavun Leposava
Janošević-Ležaić Aleksandra
Uskoković-Marković Snežana
Spectrophotometric determination of morin in strawberries and their antioxidant activity
Arhiv za farmaciju
morin
strawberries
spectrophotometry
zinc complex
title Spectrophotometric determination of morin in strawberries and their antioxidant activity
title_full Spectrophotometric determination of morin in strawberries and their antioxidant activity
title_fullStr Spectrophotometric determination of morin in strawberries and their antioxidant activity
title_full_unstemmed Spectrophotometric determination of morin in strawberries and their antioxidant activity
title_short Spectrophotometric determination of morin in strawberries and their antioxidant activity
title_sort spectrophotometric determination of morin in strawberries and their antioxidant activity
topic morin
strawberries
spectrophotometry
zinc complex
url https://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632101055P.pdf
work_keys_str_mv AT pavunleposava spectrophotometricdeterminationofmorininstrawberriesandtheirantioxidantactivity
AT janoseviclezaicaleksandra spectrophotometricdeterminationofmorininstrawberriesandtheirantioxidantactivity
AT uskokovicmarkovicsnezana spectrophotometricdeterminationofmorininstrawberriesandtheirantioxidantactivity