Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice

Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice

Abstract Background Application of the CRISPR/Cas9 system or its derived base editors enables targeted genome modification, thereby providing a programmable tool to exploit gene functions and to improve crop traits. Results We report that PmCDA1 is much more efficient than rAPOBEC1 when fused to CRI...

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Bibliographic Details
Main Authors: Wen Xu, Wei Song, Yongxing Yang, Ying Wu, Xinxin Lv, Shuang Yuan, Ya Liu, Jinxiao Yang
Format: Article
Language:English
Published: BMC 2019-11-01
Series:BMC Plant Biology
Subjects:
CRISPR/Cas9
Base editing
High-fidelity Cas9 variants
tRNA-sgRNA
Off-target effect
Online Access:http://link.springer.com/article/10.1186/s12870-019-2131-1

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