Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7
For sensitive and fast detection of <i>Escherichia coli</i> O157:H7, organic and inorganic hybrid Au/Fe<sup>3+</sup> nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and a...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/10/354 |
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| author | Yuzhi Li Fei Gao Chang Lu Marie-Laure Fauconnier Jinkai Zheng |
| author_facet | Yuzhi Li Fei Gao Chang Lu Marie-Laure Fauconnier Jinkai Zheng |
| author_sort | Yuzhi Li |
| collection | DOAJ |
| description | For sensitive and fast detection of <i>Escherichia coli</i> O157:H7, organic and inorganic hybrid Au/Fe<sup>3+</sup> nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe<sup>3+</sup> porous spongy NCs with large surface area showed excellent bio-specific capability for <i>E. coli</i> O157:H7. GNPs in Au/Fe<sup>3+</sup> NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe<sup>3+</sup> NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of <i>E. coli</i> O157:H7, which showed a good linear response (10<sup>1</sup> to 10<sup>6</sup> cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60–97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of <i>E. coli</i> O157:H7. |
| first_indexed | 2024-03-10T06:41:55Z |
| format | Article |
| id | doaj.art-eeac2b595d2545f9b089447ce274320b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T06:41:55Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-eeac2b595d2545f9b089447ce274320b2023-11-22T17:35:26ZengMDPI AGBiosensors2079-63742021-09-01111035410.3390/bios11100354Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7Yuzhi Li0Fei Gao1Chang Lu2Marie-Laure Fauconnier3Jinkai Zheng4Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLaboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Liege, 5030 Gembloux, BelgiumInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaFor sensitive and fast detection of <i>Escherichia coli</i> O157:H7, organic and inorganic hybrid Au/Fe<sup>3+</sup> nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe<sup>3+</sup> porous spongy NCs with large surface area showed excellent bio-specific capability for <i>E. coli</i> O157:H7. GNPs in Au/Fe<sup>3+</sup> NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe<sup>3+</sup> NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of <i>E. coli</i> O157:H7, which showed a good linear response (10<sup>1</sup> to 10<sup>6</sup> cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60–97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of <i>E. coli</i> O157:H7.https://www.mdpi.com/2079-6374/11/10/354SERS<i>E. coli</i> O157:H7detectionAu/Fe<sup>3+</sup> nanoclustersbiosensor |
| spellingShingle | Yuzhi Li Fei Gao Chang Lu Marie-Laure Fauconnier Jinkai Zheng Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7 Biosensors SERS <i>E. coli</i> O157:H7 detection Au/Fe<sup>3+</sup> nanoclusters biosensor |
| title | Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7 |
| title_full | Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7 |
| title_fullStr | Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7 |
| title_full_unstemmed | Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7 |
| title_short | Bio-Specific Au/Fe<sup>3+</sup> Porous Spongy Nanoclusters for Sensitive SERS Detection of <i>Escherichia coli</i> O157:H7 |
| title_sort | bio specific au fe sup 3 sup porous spongy nanoclusters for sensitive sers detection of i escherichia coli i o157 h7 |
| topic | SERS <i>E. coli</i> O157:H7 detection Au/Fe<sup>3+</sup> nanoclusters biosensor |
| url | https://www.mdpi.com/2079-6374/11/10/354 |
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