MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation
Abstract Background MicroRNAs (miRNAs) are critical regulators in osteogenesis and cartilage formation. This study was designed to investigate whether miR-532-5p plays a role in the regulation of osteoporosis. Methods Osteoporotic fractures (OP group, n = 10) or osteoarthritis without osteoporosis (...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | http://link.springer.com/article/10.1186/s12891-020-03317-y |
| _version_ | 1818574634245685248 |
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| author | Xinyu Guo Shijun Wei Feng Xu Xianhua Cai Huasong Wang Ran Ding |
| author_facet | Xinyu Guo Shijun Wei Feng Xu Xianhua Cai Huasong Wang Ran Ding |
| author_sort | Xinyu Guo |
| collection | DOAJ |
| description | Abstract Background MicroRNAs (miRNAs) are critical regulators in osteogenesis and cartilage formation. This study was designed to investigate whether miR-532-5p plays a role in the regulation of osteoporosis. Methods Osteoporotic fractures (OP group, n = 10) or osteoarthritis without osteoporosis (control group, n = 10) were selected as subjects in this study. Quantitative analysis of gene expression was performed by RT-PCR. Western blot was used to determine the expression levels of protein forkhead O1 (FOXO1). Bioinformatics analyses and luciferase reporter assay were used to verify the downstream target of miR-532-5p. Results Compared with the non-osteoporotic controls, miR-532-5p was upregulated in osteoporotic samples, and expression of miR-532-5p was downregulated in the osteogenic C2C12 cell model. Overexpression of miR-532-5p resulted in decreased expression levels of key osteoblast markers, including alkaline phosphatase (ALP), osteocalcin (OC), and collagen type I alpha 1 (COL1A1). The inhibitory results of miR-532-5p were reversed. MiR-532-5p contained a putative FOXO1 binding site. Moreover, miR-532-5p inhibited the expression of FOXO1, and overexpression of FOXO1 inhibited the effect of miR-532-5p on osteoblast markers. Conclusions MiR-532-5p can provide references to osteoporosis by regulating the expression of FOXO1 and osteoblast differentiation. MiR-532-5p might serve as a therapeutic target for osteoporosis. |
| first_indexed | 2024-12-15T00:29:09Z |
| format | Article |
| id | doaj.art-eead9b4639b5441483b979a9c6c520e4 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-15T00:29:09Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-eead9b4639b5441483b979a9c6c520e42022-12-21T22:42:05ZengBMCBMC Musculoskeletal Disorders1471-24742020-05-012111810.1186/s12891-020-03317-yMicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiationXinyu Guo0Shijun Wei1Feng Xu2Xianhua Cai3Huasong Wang4Ran Ding5Department of Orthopaedics, Guizhou Provincial Orthopaedic HospitalOrthopedic surgery of Wuhan General Hospital of People’s Liberation ArmyOrthopedic surgery of Wuhan General Hospital of People’s Liberation ArmyOrthopedic surgery of Wuhan General Hospital of People’s Liberation ArmyOrthopedic surgery of Wuhan General Hospital of People’s Liberation ArmyOrthopedic surgery of Wuhan General Hospital of People’s Liberation ArmyAbstract Background MicroRNAs (miRNAs) are critical regulators in osteogenesis and cartilage formation. This study was designed to investigate whether miR-532-5p plays a role in the regulation of osteoporosis. Methods Osteoporotic fractures (OP group, n = 10) or osteoarthritis without osteoporosis (control group, n = 10) were selected as subjects in this study. Quantitative analysis of gene expression was performed by RT-PCR. Western blot was used to determine the expression levels of protein forkhead O1 (FOXO1). Bioinformatics analyses and luciferase reporter assay were used to verify the downstream target of miR-532-5p. Results Compared with the non-osteoporotic controls, miR-532-5p was upregulated in osteoporotic samples, and expression of miR-532-5p was downregulated in the osteogenic C2C12 cell model. Overexpression of miR-532-5p resulted in decreased expression levels of key osteoblast markers, including alkaline phosphatase (ALP), osteocalcin (OC), and collagen type I alpha 1 (COL1A1). The inhibitory results of miR-532-5p were reversed. MiR-532-5p contained a putative FOXO1 binding site. Moreover, miR-532-5p inhibited the expression of FOXO1, and overexpression of FOXO1 inhibited the effect of miR-532-5p on osteoblast markers. Conclusions MiR-532-5p can provide references to osteoporosis by regulating the expression of FOXO1 and osteoblast differentiation. MiR-532-5p might serve as a therapeutic target for osteoporosis.http://link.springer.com/article/10.1186/s12891-020-03317-ymiR-532-5pFOXO1OsteoporosisOsteogenic differentiation |
| spellingShingle | Xinyu Guo Shijun Wei Feng Xu Xianhua Cai Huasong Wang Ran Ding MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation BMC Musculoskeletal Disorders miR-532-5p FOXO1 Osteoporosis Osteogenic differentiation |
| title | MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation |
| title_full | MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation |
| title_fullStr | MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation |
| title_full_unstemmed | MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation |
| title_short | MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation |
| title_sort | microrna 532 5p is implicated in the regulation of osteoporosis by forkhead box o1 and osteoblast differentiation |
| topic | miR-532-5p FOXO1 Osteoporosis Osteogenic differentiation |
| url | http://link.springer.com/article/10.1186/s12891-020-03317-y |
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