Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice
Delta-like non-canonical Notch ligand 1 (<i>DLK1</i>), which inhibits the differentiation of precursor adipocytes, is a recognized marker gene for precursor adipocytes. Lipids play a crucial role in energy storage and metabolism as a vital determinant of beef quality. In this study, we i...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/132 |
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| author | Xin Lu Xibi Fang Jiaqi Mi Yue Liu Ruimin Liu Guanghui Li Yue Li Runjun Yang |
| author_facet | Xin Lu Xibi Fang Jiaqi Mi Yue Liu Ruimin Liu Guanghui Li Yue Li Runjun Yang |
| author_sort | Xin Lu |
| collection | DOAJ |
| description | Delta-like non-canonical Notch ligand 1 (<i>DLK1</i>), which inhibits the differentiation of precursor adipocytes, is a recognized marker gene for precursor adipocytes. Lipids play a crucial role in energy storage and metabolism as a vital determinant of beef quality. In this study, we investigated the mechanism of the <i>DLK1</i> gene in lipid metabolism by constructing adipose tissue-specific knockout mice. We examined some phenotypic traits, including body weight, liver coefficient, fat index, the content of triglyceride (TG) and cholesterol (CHOL) in abdominal white adipose tissue (WAT) and blood. Subsequently, the fatty acid content and genes related to lipid metabolism expression were detected in <i>DLK1</i><sup>−/−</sup> and wild-type mice via GC-MS/MS analysis and quantitative real-time PCR (qRT-PCR), respectively. The results illustrated that <i>DLK1</i><sup>−/−</sup> mice exhibited significant abdominal fat deposition compared to wild-type mice. HE staining and immunohistochemistry (IHC) results showed that the white adipocytes of <i>DLK1</i><sup>−/−</sup> mice were larger, and the protein expression level of <i>DLK1</i><sup>−/−</sup> was significantly lower. Regarding the blood biochemical parameters of female mice, <i>DLK1</i><sup>−/−</sup> mice had a strikingly higher triglyceride content (<i>p</i> < 0.001). The fatty acid content in <i>DLK1</i><sup>−/−</sup> mice was generally reduced. There was a significant reduction in the expression levels of the majority of genes that play a crucial role in lipid metabolism. This study reveals the molecular regulatory mechanism of fat metabolism in mice and provides a molecular basis and reference for the future application of the <i>DLK1</i> gene in the breeding of beef cattle with an excellent meat quality traits. It also provides a molecular basis for unravelling the complex and subtle relationship between adipose tissue and health. |
| first_indexed | 2024-03-08T15:06:54Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:06:54Z |
| publishDate | 2023-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eeb1a65fcd974fd3977b4e8d318e419f2024-01-10T14:58:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125113210.3390/ijms25010132Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in MiceXin Lu0Xibi Fang1Jiaqi Mi2Yue Liu3Ruimin Liu4Guanghui Li5Yue Li6Runjun Yang7College of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaDelta-like non-canonical Notch ligand 1 (<i>DLK1</i>), which inhibits the differentiation of precursor adipocytes, is a recognized marker gene for precursor adipocytes. Lipids play a crucial role in energy storage and metabolism as a vital determinant of beef quality. In this study, we investigated the mechanism of the <i>DLK1</i> gene in lipid metabolism by constructing adipose tissue-specific knockout mice. We examined some phenotypic traits, including body weight, liver coefficient, fat index, the content of triglyceride (TG) and cholesterol (CHOL) in abdominal white adipose tissue (WAT) and blood. Subsequently, the fatty acid content and genes related to lipid metabolism expression were detected in <i>DLK1</i><sup>−/−</sup> and wild-type mice via GC-MS/MS analysis and quantitative real-time PCR (qRT-PCR), respectively. The results illustrated that <i>DLK1</i><sup>−/−</sup> mice exhibited significant abdominal fat deposition compared to wild-type mice. HE staining and immunohistochemistry (IHC) results showed that the white adipocytes of <i>DLK1</i><sup>−/−</sup> mice were larger, and the protein expression level of <i>DLK1</i><sup>−/−</sup> was significantly lower. Regarding the blood biochemical parameters of female mice, <i>DLK1</i><sup>−/−</sup> mice had a strikingly higher triglyceride content (<i>p</i> < 0.001). The fatty acid content in <i>DLK1</i><sup>−/−</sup> mice was generally reduced. There was a significant reduction in the expression levels of the majority of genes that play a crucial role in lipid metabolism. This study reveals the molecular regulatory mechanism of fat metabolism in mice and provides a molecular basis and reference for the future application of the <i>DLK1</i> gene in the breeding of beef cattle with an excellent meat quality traits. It also provides a molecular basis for unravelling the complex and subtle relationship between adipose tissue and health.https://www.mdpi.com/1422-0067/25/1/132<i>DLK 1</i>adipose tissue-specific knockout micetriglyceridelipid metabolism |
| spellingShingle | Xin Lu Xibi Fang Jiaqi Mi Yue Liu Ruimin Liu Guanghui Li Yue Li Runjun Yang Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice International Journal of Molecular Sciences <i>DLK 1</i> adipose tissue-specific knockout mice triglyceride lipid metabolism |
| title | Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice |
| title_full | Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice |
| title_fullStr | Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice |
| title_full_unstemmed | Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice |
| title_short | Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice |
| title_sort | effects of adipose tissue specific knockout of delta like non canonical notch ligand 1 on lipid metabolism in mice |
| topic | <i>DLK 1</i> adipose tissue-specific knockout mice triglyceride lipid metabolism |
| url | https://www.mdpi.com/1422-0067/25/1/132 |
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