miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro Study
Introduction: Oral squamous cell carcinoma (OSCC) is one of the many cancer types where microRNAs (miRs) are often found to be overexpressed. STAT3, a significant component of human cancer, is now well recognized in recent research and is regarded as an attractive target for the creation of novel an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Orofacial Sciences |
| Subjects: | |
| Online Access: | http://www.jofs.in/article.asp?issn=0975-8844;year=2023;volume=15;issue=2;spage=167;epage=174;aulast=Thomas |
| _version_ | 1797300055083843584 |
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| author | Priya Thomas Sushmaa Chandralekha Selvakumar K. Auxzilia Preethi Abilasha Ramasubramanian Pratibha Ramani Durairaj Sekar |
| author_facet | Priya Thomas Sushmaa Chandralekha Selvakumar K. Auxzilia Preethi Abilasha Ramasubramanian Pratibha Ramani Durairaj Sekar |
| author_sort | Priya Thomas |
| collection | DOAJ |
| description | Introduction: Oral squamous cell carcinoma (OSCC) is one of the many cancer types where microRNAs (miRs) are often found to be overexpressed. STAT3, a significant component of human cancer, is now well recognized in recent research and is regarded as an attractive target for the creation of novel anti-cancer medications. We assessed the expression, functions, and mechanisms of miR-20a-3p and STAT3 in the regulation of OSCC cell proliferation, migration, and apoptosis to highlight the significance of miRNA dysregulation in cancer etiology. Materials and Methods: miR-20a-3p’s function was examined by transfecting KB cells with the miR-20a-3p and STAT3 plasmids, followed by cell proliferation (CCK-8) assays, migration, and apoptosis. Furthermore, the impact of miR-20a-3p on the expression of its target gene was investigated using a quantitative real-time polymerase chain reaction. The expression of miR-20a-3p, STAT3, and IL-6 was investigated using a quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The findings indicated that miR-20a-3p was downregulated ad STAT3 was upregulated in OSCC cells. Elevated STAT3 levels in OSCC cells were associated with enhanced tumor cell proliferation, migration, decreased apoptosis, and upregulated IL-6 expression. In OSCC cells, the overexpression of miR-20a-3p was accompanied by a reduction in the production of STAT3 and IL-6. Conclusion: In conclusion, our work showed that miR-20a-3p served as a tumor suppressor in OSCC by reducing the proliferation and migration of cancer cells by inhibiting STAT3 expression. |
| first_indexed | 2024-03-07T23:00:40Z |
| format | Article |
| id | doaj.art-eeb72f1055d84b70a86c9a1e2382f7c1 |
| institution | Directory Open Access Journal |
| issn | 0975-8844 |
| language | English |
| last_indexed | 2024-03-07T23:00:40Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Orofacial Sciences |
| spelling | doaj.art-eeb72f1055d84b70a86c9a1e2382f7c12024-02-22T15:03:50ZengWolters Kluwer Medknow PublicationsJournal of Orofacial Sciences0975-88442023-01-0115216717410.4103/jofs.jofs_330_23miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro StudyPriya ThomasSushmaa Chandralekha SelvakumarK. Auxzilia PreethiAbilasha RamasubramanianPratibha RamaniDurairaj SekarIntroduction: Oral squamous cell carcinoma (OSCC) is one of the many cancer types where microRNAs (miRs) are often found to be overexpressed. STAT3, a significant component of human cancer, is now well recognized in recent research and is regarded as an attractive target for the creation of novel anti-cancer medications. We assessed the expression, functions, and mechanisms of miR-20a-3p and STAT3 in the regulation of OSCC cell proliferation, migration, and apoptosis to highlight the significance of miRNA dysregulation in cancer etiology. Materials and Methods: miR-20a-3p’s function was examined by transfecting KB cells with the miR-20a-3p and STAT3 plasmids, followed by cell proliferation (CCK-8) assays, migration, and apoptosis. Furthermore, the impact of miR-20a-3p on the expression of its target gene was investigated using a quantitative real-time polymerase chain reaction. The expression of miR-20a-3p, STAT3, and IL-6 was investigated using a quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The findings indicated that miR-20a-3p was downregulated ad STAT3 was upregulated in OSCC cells. Elevated STAT3 levels in OSCC cells were associated with enhanced tumor cell proliferation, migration, decreased apoptosis, and upregulated IL-6 expression. In OSCC cells, the overexpression of miR-20a-3p was accompanied by a reduction in the production of STAT3 and IL-6. Conclusion: In conclusion, our work showed that miR-20a-3p served as a tumor suppressor in OSCC by reducing the proliferation and migration of cancer cells by inhibiting STAT3 expression.http://www.jofs.in/article.asp?issn=0975-8844;year=2023;volume=15;issue=2;spage=167;epage=174;aulast=Thomasmir-20amigrationoral squamous cell carcinomastat3 |
| spellingShingle | Priya Thomas Sushmaa Chandralekha Selvakumar K. Auxzilia Preethi Abilasha Ramasubramanian Pratibha Ramani Durairaj Sekar miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro Study Journal of Orofacial Sciences mir-20a migration oral squamous cell carcinoma stat3 |
| title | miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro Study |
| title_full | miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro Study |
| title_fullStr | miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro Study |
| title_full_unstemmed | miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro Study |
| title_short | miRNA-20a: A Dual Regulator of Cell Migration and Apoptosis in Oral Squamous Cell Carcinoma:– An In Vitro Study |
| title_sort | mirna 20a a dual regulator of cell migration and apoptosis in oral squamous cell carcinoma an in vitro study |
| topic | mir-20a migration oral squamous cell carcinoma stat3 |
| url | http://www.jofs.in/article.asp?issn=0975-8844;year=2023;volume=15;issue=2;spage=167;epage=174;aulast=Thomas |
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