A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice
Background: The development of anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis following viral encephalitis, such as Japanese encephalitis, has received increasing attention in recent years. However, the mechanism of anti-NMDAR antibody production following Japanese encephalitis has not been...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402400731X |
| _version_ | 1827360855845502976 |
|---|---|
| author | Hanyu Luo Jiaxin Yang Xiaoyue Yang Ziyao Han Zhixu Fang Dishu Huang Jianxiong Gui Ran Ding Hengsheng Chen Li Cheng Jiannan Ma Li Jiang |
| author_facet | Hanyu Luo Jiaxin Yang Xiaoyue Yang Ziyao Han Zhixu Fang Dishu Huang Jianxiong Gui Ran Ding Hengsheng Chen Li Cheng Jiannan Ma Li Jiang |
| author_sort | Hanyu Luo |
| collection | DOAJ |
| description | Background: The development of anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis following viral encephalitis, such as Japanese encephalitis, has received increasing attention in recent years. However, the mechanism of anti-NMDAR antibody production following Japanese encephalitis has not been explored. Methods: A peptide from the Japanese encephalitis virus (JEV), which shares a similar amino acid sequence with GluN1, was identified by sequence comparison. We then explored whether active subcutaneous immunization with the JEV peptide could induce the production of anti-NMDAR antibodies and related pathophysiological and behavioral changes in mice. In addition, a published active immune model of anti-NMDAR encephalitis using a GluN1 peptide was used as the positive control. Results: A 6-amino-acid sequence with 83 % similarity between the envelope protein of the JEV (HGTVVI) and GluN1 (NGTHVI) was identified, and the sequence included the N368/G369 region. Active immunization with the JEV peptide induced a substantial and specific immune response in mice. However, anti-NMDAR antibodies were not detected in the serum of mice immunized with the JEV peptide by ELISA, CBA, and TBA. Moreover, mice immunized with the JEV peptide presented no abnormities related to anti-NMDAR antibodies according to western blotting, patch clamp, and a series of behavioral tests. In addition, active immunization with a recently reported GluN1 peptide failed to induce anti-NMDAR antibody production in mice. Conclusions: In this study, the attempt of active immunization with the JEV peptide to induce the production of anti-NMDAR antibodies via molecular mimicry failed. The pathogenesis of anti-NMDAR encephalitis following Japanese encephalitis remains to be elucidated. |
| first_indexed | 2024-03-08T06:54:29Z |
| format | Article |
| id | doaj.art-eeb7b1ad18a643478d408d29253c1a9e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T06:54:29Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-eeb7b1ad18a643478d408d29253c1a9e2024-02-03T06:38:15ZengElsevierHeliyon2405-84402024-01-01102e24700A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in miceHanyu Luo0Jiaxin Yang1Xiaoyue Yang2Ziyao Han3Zhixu Fang4Dishu Huang5Jianxiong Gui6Ran Ding7Hengsheng Chen8Li Cheng9Jiannan Ma10Li Jiang11Department of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, ChinaDepartment of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, China; Corresponding author. No. 136 Zhongshan 2nd Road, Yuzhong District, Chongqing, 400014, China.Department of Neurology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Chongqing, China; Corresponding author. No. 136 Zhongshan 2nd Road, Yuzhong District, Chongqing, 400014, China.Background: The development of anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis following viral encephalitis, such as Japanese encephalitis, has received increasing attention in recent years. However, the mechanism of anti-NMDAR antibody production following Japanese encephalitis has not been explored. Methods: A peptide from the Japanese encephalitis virus (JEV), which shares a similar amino acid sequence with GluN1, was identified by sequence comparison. We then explored whether active subcutaneous immunization with the JEV peptide could induce the production of anti-NMDAR antibodies and related pathophysiological and behavioral changes in mice. In addition, a published active immune model of anti-NMDAR encephalitis using a GluN1 peptide was used as the positive control. Results: A 6-amino-acid sequence with 83 % similarity between the envelope protein of the JEV (HGTVVI) and GluN1 (NGTHVI) was identified, and the sequence included the N368/G369 region. Active immunization with the JEV peptide induced a substantial and specific immune response in mice. However, anti-NMDAR antibodies were not detected in the serum of mice immunized with the JEV peptide by ELISA, CBA, and TBA. Moreover, mice immunized with the JEV peptide presented no abnormities related to anti-NMDAR antibodies according to western blotting, patch clamp, and a series of behavioral tests. In addition, active immunization with a recently reported GluN1 peptide failed to induce anti-NMDAR antibody production in mice. Conclusions: In this study, the attempt of active immunization with the JEV peptide to induce the production of anti-NMDAR antibodies via molecular mimicry failed. The pathogenesis of anti-NMDAR encephalitis following Japanese encephalitis remains to be elucidated.http://www.sciencedirect.com/science/article/pii/S240584402400731XAnti-NMDAR encephalitisJapanese encephalitisActive immunizationMolecular mimicryPeptide |
| spellingShingle | Hanyu Luo Jiaxin Yang Xiaoyue Yang Ziyao Han Zhixu Fang Dishu Huang Jianxiong Gui Ran Ding Hengsheng Chen Li Cheng Jiannan Ma Li Jiang A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice Heliyon Anti-NMDAR encephalitis Japanese encephalitis Active immunization Molecular mimicry Peptide |
| title | A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice |
| title_full | A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice |
| title_fullStr | A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice |
| title_full_unstemmed | A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice |
| title_short | A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice |
| title_sort | peptide from the japanese encephalitis virus failed to induce the production of anti n methyl d aspartate receptor antibodies via molecular mimicry in mice |
| topic | Anti-NMDAR encephalitis Japanese encephalitis Active immunization Molecular mimicry Peptide |
| url | http://www.sciencedirect.com/science/article/pii/S240584402400731X |
| work_keys_str_mv | AT hanyuluo apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT jiaxinyang apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT xiaoyueyang apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT ziyaohan apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT zhixufang apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT dishuhuang apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT jianxionggui apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT randing apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT hengshengchen apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT licheng apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT jiannanma apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT lijiang apeptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT hanyuluo peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT jiaxinyang peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT xiaoyueyang peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT ziyaohan peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT zhixufang peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT dishuhuang peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT jianxionggui peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT randing peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT hengshengchen peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT licheng peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT jiannanma peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice AT lijiang peptidefromthejapaneseencephalitisvirusfailedtoinducetheproductionofantinmethyldaspartatereceptorantibodiesviamolecularmimicryinmice |