Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets
Nutrients in the maternal diet favor the growth and development of suckling piglets and alter their gut microbiota composition and metabolic activity, thus affecting the hosts. The present study analyzed, in suckling piglets from sows receiving antibiotic or synbiotic supplements from pregnancy to l...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-11-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.575685/full |
| _version_ | 1818602665123250176 |
|---|---|
| author | Cui Ma Cui Ma Qiankun Gao Wanghong Zhang Wanghong Zhang Qian Zhu Qian Zhu Wu Tang Wu Tang Francois Blachier Hao Ding Xiangfeng Kong |
| author_facet | Cui Ma Cui Ma Qiankun Gao Wanghong Zhang Wanghong Zhang Qian Zhu Qian Zhu Wu Tang Wu Tang Francois Blachier Hao Ding Xiangfeng Kong |
| author_sort | Cui Ma |
| collection | DOAJ |
| description | Nutrients in the maternal diet favor the growth and development of suckling piglets and alter their gut microbiota composition and metabolic activity, thus affecting the hosts. The present study analyzed, in suckling piglets from sows receiving antibiotic or synbiotic supplements from pregnancy to lactation, several biochemical parameters, oxidative/anti-oxidative indices, inflammatory cytokines, and ingestion-related factor levels in plasma, as well as colonic microbiota composition and metabolic activity, and mucosal expression of genes related to the intestinal barrier function. Compared with the control group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, AMM, TC, low-density lipoprotein-cholesterol (LDL-C), MDA, H2O2, ghrelin, CCK, PP, IL-1β, IL-2, IL-6, TNF-α, Ala, Cys, Tau, and β-AiBA, the levels of propionate and total short-chain fatty acids (SCFAs) in the colonic luminal content, and colonic abundances of RFN20, Anaerostipes, and Butyricimonas; while increased (P < 0.05) the plasma levels of urea nitrogen (UN), Ile, Leu, α-AAA, α-ABA, and 1-Mehis, as well as colonic abundances of Sphingomonas, Anaerovorax, Sharpea, and Butyricicoccus. Compared with the antibiotic group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, gastrin, and Ala, as well as abundances of Pasteurella and RFN20 and propionate level in the colonic content. Expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α were down-regulated in the colonic mucosa. The synbiotic supplementation increased (P < 0.05) the plasma levels of UN, Leu, α-ABA, and 1-Mehis, the abundances of Anaerovorax, Sharpea, and Butyricicoccus and expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α. Spearman correlation analysis showed that there was a positive correlation between colonic Anaerostipes abundance and acetate and SCFAs levels; whereas a negative correlation between Fusobacteria and Fusobacterium abundances and acetate level. These findings suggest that synbiotic supplementation in the maternal diet improved nutrient metabolism and intestinal barrier permeability, reduced oxidative stress, and modified colonic microbiota composition and metabolic activity in suckling piglets. |
| first_indexed | 2024-12-16T13:10:53Z |
| format | Article |
| id | doaj.art-eebd7a934a134703b4c55f3c4c8e30ee |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-16T13:10:53Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-eebd7a934a134703b4c55f3c4c8e30ee2022-12-21T22:30:37ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-11-01710.3389/fvets.2020.575685575685Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling PigletsCui Ma0Cui Ma1Qiankun Gao2Wanghong Zhang3Wanghong Zhang4Qian Zhu5Qian Zhu6Wu Tang7Wu Tang8Francois Blachier9Hao Ding10Xiangfeng Kong11CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaUniversité Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, Paris, FranceCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaCAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaNutrients in the maternal diet favor the growth and development of suckling piglets and alter their gut microbiota composition and metabolic activity, thus affecting the hosts. The present study analyzed, in suckling piglets from sows receiving antibiotic or synbiotic supplements from pregnancy to lactation, several biochemical parameters, oxidative/anti-oxidative indices, inflammatory cytokines, and ingestion-related factor levels in plasma, as well as colonic microbiota composition and metabolic activity, and mucosal expression of genes related to the intestinal barrier function. Compared with the control group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, AMM, TC, low-density lipoprotein-cholesterol (LDL-C), MDA, H2O2, ghrelin, CCK, PP, IL-1β, IL-2, IL-6, TNF-α, Ala, Cys, Tau, and β-AiBA, the levels of propionate and total short-chain fatty acids (SCFAs) in the colonic luminal content, and colonic abundances of RFN20, Anaerostipes, and Butyricimonas; while increased (P < 0.05) the plasma levels of urea nitrogen (UN), Ile, Leu, α-AAA, α-ABA, and 1-Mehis, as well as colonic abundances of Sphingomonas, Anaerovorax, Sharpea, and Butyricicoccus. Compared with the antibiotic group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, gastrin, and Ala, as well as abundances of Pasteurella and RFN20 and propionate level in the colonic content. Expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α were down-regulated in the colonic mucosa. The synbiotic supplementation increased (P < 0.05) the plasma levels of UN, Leu, α-ABA, and 1-Mehis, the abundances of Anaerovorax, Sharpea, and Butyricicoccus and expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α. Spearman correlation analysis showed that there was a positive correlation between colonic Anaerostipes abundance and acetate and SCFAs levels; whereas a negative correlation between Fusobacteria and Fusobacterium abundances and acetate level. These findings suggest that synbiotic supplementation in the maternal diet improved nutrient metabolism and intestinal barrier permeability, reduced oxidative stress, and modified colonic microbiota composition and metabolic activity in suckling piglets.https://www.frontiersin.org/articles/10.3389/fvets.2020.575685/fullbiochemical parametersgut microbiotametabolitessowssuckling pigletssynbiotic |
| spellingShingle | Cui Ma Cui Ma Qiankun Gao Wanghong Zhang Wanghong Zhang Qian Zhu Qian Zhu Wu Tang Wu Tang Francois Blachier Hao Ding Xiangfeng Kong Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets Frontiers in Veterinary Science biochemical parameters gut microbiota metabolites sows suckling piglets synbiotic |
| title | Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets |
| title_full | Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets |
| title_fullStr | Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets |
| title_full_unstemmed | Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets |
| title_short | Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets |
| title_sort | supplementing synbiotic in sows diets modifies beneficially blood parameters and colonic microbiota composition and metabolic activity in suckling piglets |
| topic | biochemical parameters gut microbiota metabolites sows suckling piglets synbiotic |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2020.575685/full |
| work_keys_str_mv | AT cuima supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT cuima supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT qiankungao supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT wanghongzhang supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT wanghongzhang supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT qianzhu supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT qianzhu supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT wutang supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT wutang supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT francoisblachier supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT haoding supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets AT xiangfengkong supplementingsynbioticinsowsdietsmodifiesbeneficiallybloodparametersandcolonicmicrobiotacompositionandmetabolicactivityinsucklingpiglets |