Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical Carcinoma
BackgroundAdrenal cortical carcinoma (ACC) is a rare cancer with a variable prognosis. Several prognostic factors of ACC have been previously reported, but a proteomic analysis has not yet been performed. This study aimed to investigate prognostic biomarkers for ACC using a proteomic approach.Method...
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| Format: | Article |
| Language: | English |
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Korean Endocrine Society
2020-03-01
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| Series: | Endocrinology and Metabolism |
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| Online Access: | https://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-35-132.pdf |
| _version_ | 1819049527563255808 |
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| author | Sun Joon Moon Jung Hee Kim Sung Hye Kong Chan Soo Shin |
| author_facet | Sun Joon Moon Jung Hee Kim Sung Hye Kong Chan Soo Shin |
| author_sort | Sun Joon Moon |
| collection | DOAJ |
| description | BackgroundAdrenal cortical carcinoma (ACC) is a rare cancer with a variable prognosis. Several prognostic factors of ACC have been previously reported, but a proteomic analysis has not yet been performed. This study aimed to investigate prognostic biomarkers for ACC using a proteomic approach.MethodsWe used reverse-phase protein array data from The Cancer Proteome Atlas, and identified differentially expressed proteins in metastatic ACCs. Multivariate Cox regression analysis adjusted by age and staging was used for survival analysis, and the C-index and category-free net reclassification improvement (cfNRI) were utilized to evaluate additive prognostic value.ResultsIn 46 patients with ACC, cyclin B1, transferrin receptor (TfR1), and fibronectin were significantly overexpressed in patients with distant metastasis. In multivariate models, high expression of cyclin B1 and TfR1 was significantly associated with mortality (hazard ratio [HR], 6.13; 95% confidence interval [CI], 1.02 to 36.7; and HR, 6.59; 95% CI, 1.14 to 38.2; respectively), whereas high fibronectin expression was not (HR, 3.92; 95% CI, 0.75 to 20.4). Combinations of high cyclin B1/high TfR1, high cyclin B1/high fibronectin, and high TfR1/high fibronectin were strongly associated with mortality ([HR, 13.72; 95% CI, 1.89 to 99.66], [HR, 9.22; 95% CI, 1.34 to 63.55], and [HR, 18.59; 95% CI, 2.54 to 135.88], respectively). In reclassification analyses, cyclin B1, TfR1, fibronectin, and combinations thereof improved the prognostic performance (C-index, 0.78 to 0.82–0.86; cfNRI, all P values <0.05).ConclusionIn ACC patients, the overexpression of cyclin B1, TfR1, and fibronectin and combinations thereof were associated with poor prognosis. |
| first_indexed | 2024-12-21T11:33:34Z |
| format | Article |
| id | doaj.art-eec52a1a8d3249778e50f886c8701fd7 |
| institution | Directory Open Access Journal |
| issn | 2093-596X 2093-5978 |
| language | English |
| last_indexed | 2024-12-21T11:33:34Z |
| publishDate | 2020-03-01 |
| publisher | Korean Endocrine Society |
| record_format | Article |
| series | Endocrinology and Metabolism |
| spelling | doaj.art-eec52a1a8d3249778e50f886c8701fd72022-12-21T19:05:30ZengKorean Endocrine SocietyEndocrinology and Metabolism2093-596X2093-59782020-03-0135113214110.3803/EnM.2020.35.1.132Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical CarcinomaSun Joon Moon0Jung Hee Kim1Sung Hye Kong2Chan Soo Shin3Department of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, .KoreaDepartment of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, .KoreaDepartment of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, .KoreaDepartment of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, .KoreaBackgroundAdrenal cortical carcinoma (ACC) is a rare cancer with a variable prognosis. Several prognostic factors of ACC have been previously reported, but a proteomic analysis has not yet been performed. This study aimed to investigate prognostic biomarkers for ACC using a proteomic approach.MethodsWe used reverse-phase protein array data from The Cancer Proteome Atlas, and identified differentially expressed proteins in metastatic ACCs. Multivariate Cox regression analysis adjusted by age and staging was used for survival analysis, and the C-index and category-free net reclassification improvement (cfNRI) were utilized to evaluate additive prognostic value.ResultsIn 46 patients with ACC, cyclin B1, transferrin receptor (TfR1), and fibronectin were significantly overexpressed in patients with distant metastasis. In multivariate models, high expression of cyclin B1 and TfR1 was significantly associated with mortality (hazard ratio [HR], 6.13; 95% confidence interval [CI], 1.02 to 36.7; and HR, 6.59; 95% CI, 1.14 to 38.2; respectively), whereas high fibronectin expression was not (HR, 3.92; 95% CI, 0.75 to 20.4). Combinations of high cyclin B1/high TfR1, high cyclin B1/high fibronectin, and high TfR1/high fibronectin were strongly associated with mortality ([HR, 13.72; 95% CI, 1.89 to 99.66], [HR, 9.22; 95% CI, 1.34 to 63.55], and [HR, 18.59; 95% CI, 2.54 to 135.88], respectively). In reclassification analyses, cyclin B1, TfR1, fibronectin, and combinations thereof improved the prognostic performance (C-index, 0.78 to 0.82–0.86; cfNRI, all P values <0.05).ConclusionIn ACC patients, the overexpression of cyclin B1, TfR1, and fibronectin and combinations thereof were associated with poor prognosis.https://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-35-132.pdfadrenocortical carcinomaprotein array analysisproteomicsprognosis |
| spellingShingle | Sun Joon Moon Jung Hee Kim Sung Hye Kong Chan Soo Shin Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical Carcinoma Endocrinology and Metabolism adrenocortical carcinoma protein array analysis proteomics prognosis |
| title | Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical Carcinoma |
| title_full | Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical Carcinoma |
| title_fullStr | Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical Carcinoma |
| title_full_unstemmed | Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical Carcinoma |
| title_short | Protein Expression of Cyclin B1, Transferrin Receptor, and Fibronectin Is Correlated with the Prognosis of Adrenal Cortical Carcinoma |
| title_sort | protein expression of cyclin b1 transferrin receptor and fibronectin is correlated with the prognosis of adrenal cortical carcinoma |
| topic | adrenocortical carcinoma protein array analysis proteomics prognosis |
| url | https://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-35-132.pdf |
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