Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer
Abstract Background Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. Methods The expr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13000-020-00946-8 |
| _version_ | 1818978929497604096 |
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| author | Ying Li Yusong Liu Jie Yao Rui Li Xiaocheng Fan |
| author_facet | Ying Li Yusong Liu Jie Yao Rui Li Xiaocheng Fan |
| author_sort | Ying Li |
| collection | DOAJ |
| description | Abstract Background Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. Methods The expression of miR-484 in gastric cancer was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were employed to explore the prognostic significance of miR-484 in gastric cancer. The functional role of miR-484 in gastric cancer was determined by CCK-8 and Transwell assays. Results The results showed that miR-484 was significantly downregulated in gastric cancer tissues and cell lines. The downregulation of miR-484 was closely related to differentiation, lymph node metastasis, TNM stage, and poor prognosis. Cox regression analyses demonstrated that miR-484 was an independent prognosis indicator for gastric cancer patients. Additionally, the downregulation of miR-484 enhanced cell proliferation, migration, and invasion in gastric cancer cells. Conclusion These data demonstrated that miR-484 can serve as a potential prognostic biomarker and therapeutic target for gastric cancer and it may be involved in the progression of gastric cancer. |
| first_indexed | 2024-12-20T16:51:27Z |
| format | Article |
| id | doaj.art-eec554b8d4c7413b9f29c8ef1108c161 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-12-20T16:51:27Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-eec554b8d4c7413b9f29c8ef1108c1612022-12-21T19:32:48ZengBMCDiagnostic Pathology1746-15962020-03-011511710.1186/s13000-020-00946-8Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancerYing Li0Yusong Liu1Jie Yao2Rui Li3Xiaocheng Fan4Department of Oncology, Jining Hospital of Traditional Chinese MedicineDepartment of Oncology, Jining Hospital of Traditional Chinese MedicineDepartment of Oncology, Jining Hospital of Traditional Chinese MedicineDepartment of Oncology, Jining Hospital of Traditional Chinese MedicineDepartment of Oncology, Jining Hospital of Traditional Chinese MedicineAbstract Background Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. Methods The expression of miR-484 in gastric cancer was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were employed to explore the prognostic significance of miR-484 in gastric cancer. The functional role of miR-484 in gastric cancer was determined by CCK-8 and Transwell assays. Results The results showed that miR-484 was significantly downregulated in gastric cancer tissues and cell lines. The downregulation of miR-484 was closely related to differentiation, lymph node metastasis, TNM stage, and poor prognosis. Cox regression analyses demonstrated that miR-484 was an independent prognosis indicator for gastric cancer patients. Additionally, the downregulation of miR-484 enhanced cell proliferation, migration, and invasion in gastric cancer cells. Conclusion These data demonstrated that miR-484 can serve as a potential prognostic biomarker and therapeutic target for gastric cancer and it may be involved in the progression of gastric cancer.http://link.springer.com/article/10.1186/s13000-020-00946-8Gastric cancermicroRNA-484ProliferationMigrationInvasionPrognosis |
| spellingShingle | Ying Li Yusong Liu Jie Yao Rui Li Xiaocheng Fan Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer Diagnostic Pathology Gastric cancer microRNA-484 Proliferation Migration Invasion Prognosis |
| title | Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer |
| title_full | Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer |
| title_fullStr | Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer |
| title_full_unstemmed | Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer |
| title_short | Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer |
| title_sort | downregulation of mir 484 is associated with poor prognosis and tumor progression of gastric cancer |
| topic | Gastric cancer microRNA-484 Proliferation Migration Invasion Prognosis |
| url | http://link.springer.com/article/10.1186/s13000-020-00946-8 |
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