Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential.
Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3216982?pdf=render |
| _version_ | 1818136516822564864 |
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| author | Martina Adamek Jörg Overhage Stephan Bathe Josef Winter Reinhard Fischer Thomas Schwartz |
| author_facet | Martina Adamek Jörg Overhage Stephan Bathe Josef Winter Reinhard Fischer Thomas Schwartz |
| author_sort | Martina Adamek |
| collection | DOAJ |
| description | Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequencing distinguished 10 subgroups. For 8 of them, the same strain composition was shown with both typing methods. A subset of 59 isolates representative for the gyrB groups was further investigated with regards to their pathogenic properties in a virulence model using Dictyostelium discoideum and Acanthamoeba castellanii as host organisms. A clear tendency towards accumulation of virulent strains could be observed for one group with A. castellanii and for two groups with D. discoideum. Several virulent strains did not cluster in any of the genetic groups, while other groups displayed no virulence properties at all. The amoeba pathogenicity model proved suitable in showing differences in S. maltophilia virulence. However, the model is still not sufficient to completely elucidate virulence as critical for a human host, since several strains involved in human infections did not show any virulence against amoeba. |
| first_indexed | 2024-12-11T09:41:39Z |
| format | Article |
| id | doaj.art-eed13ff831784fbaa309087b924bcf28 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T09:41:39Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-eed13ff831784fbaa309087b924bcf282022-12-22T01:12:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2761510.1371/journal.pone.0027615Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential.Martina AdamekJörg OverhageStephan BatheJosef WinterReinhard FischerThomas SchwartzStenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequencing distinguished 10 subgroups. For 8 of them, the same strain composition was shown with both typing methods. A subset of 59 isolates representative for the gyrB groups was further investigated with regards to their pathogenic properties in a virulence model using Dictyostelium discoideum and Acanthamoeba castellanii as host organisms. A clear tendency towards accumulation of virulent strains could be observed for one group with A. castellanii and for two groups with D. discoideum. Several virulent strains did not cluster in any of the genetic groups, while other groups displayed no virulence properties at all. The amoeba pathogenicity model proved suitable in showing differences in S. maltophilia virulence. However, the model is still not sufficient to completely elucidate virulence as critical for a human host, since several strains involved in human infections did not show any virulence against amoeba.http://europepmc.org/articles/PMC3216982?pdf=render |
| spellingShingle | Martina Adamek Jörg Overhage Stephan Bathe Josef Winter Reinhard Fischer Thomas Schwartz Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential. PLoS ONE |
| title | Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential. |
| title_full | Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential. |
| title_fullStr | Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential. |
| title_full_unstemmed | Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential. |
| title_short | Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential. |
| title_sort | genotyping of environmental and clinical stenotrophomonas maltophilia isolates and their pathogenic potential |
| url | http://europepmc.org/articles/PMC3216982?pdf=render |
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