Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders
Spermatogenesis is temperature-dependent, and the increase in testicular temperature seriously affects mammalian spermatogenesis and semen quality. In this study, the testicular heat stress model of mice was made with a 43 °C water bath for 25 min, and the effects of heat stress on semen quality and...
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| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8809 |
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| author | Mailin Gan Yunhong Jing Zhongwei Xie Jianfeng Ma Lei Chen Shunhua Zhang Ye Zhao Lili Niu Yan Wang Xuewei Li Li Zhu Linyuan Shen |
| author_facet | Mailin Gan Yunhong Jing Zhongwei Xie Jianfeng Ma Lei Chen Shunhua Zhang Ye Zhao Lili Niu Yan Wang Xuewei Li Li Zhu Linyuan Shen |
| author_sort | Mailin Gan |
| collection | DOAJ |
| description | Spermatogenesis is temperature-dependent, and the increase in testicular temperature seriously affects mammalian spermatogenesis and semen quality. In this study, the testicular heat stress model of mice was made with a 43 °C water bath for 25 min, and the effects of heat stress on semen quality and spermatogenesis-related regulators were analyzed. On the 7th day after heat stress, testis weight shrank to 68.45% and sperm density dropped to 33.20%. High-throughput sequencing analysis showed that 98 microRNAs (miRNAs) and 369 mRNAs were down-regulated, while 77 miRNAs and 1424 mRNAs were up-regulated after heat stress. Through gene ontology (GO) analysis of differentially expressed genes and miRNA–mRNA co-expression networks, it was found that heat stress may be involved in the regulation of testicular atrophy and spermatogenesis disorders by affecting cell meiosis process and cell cycle. In addition, through functional enrichment analysis, co-expression regulatory network, correlation analysis and in vitro experiment, it was found that miR-143-3p may be a representative potential key regulatory factor affecting spermatogenesis under heat stress. In summary, our results enrich the understanding of miRNAs in testicular heat stress and provide a reference for the prevention and treatment of heat-stress-induced spermatogenesis disorders. |
| first_indexed | 2024-03-11T03:40:07Z |
| format | Article |
| id | doaj.art-eed289ff17aa4fd3849f4b9f8db1e222 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:40:07Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eed289ff17aa4fd3849f4b9f8db1e2222023-11-18T01:42:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410880910.3390/ijms24108809Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis DisordersMailin Gan0Yunhong Jing1Zhongwei Xie2Jianfeng Ma3Lei Chen4Shunhua Zhang5Ye Zhao6Lili Niu7Yan Wang8Xuewei Li9Li Zhu10Linyuan Shen11Key Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaSpermatogenesis is temperature-dependent, and the increase in testicular temperature seriously affects mammalian spermatogenesis and semen quality. In this study, the testicular heat stress model of mice was made with a 43 °C water bath for 25 min, and the effects of heat stress on semen quality and spermatogenesis-related regulators were analyzed. On the 7th day after heat stress, testis weight shrank to 68.45% and sperm density dropped to 33.20%. High-throughput sequencing analysis showed that 98 microRNAs (miRNAs) and 369 mRNAs were down-regulated, while 77 miRNAs and 1424 mRNAs were up-regulated after heat stress. Through gene ontology (GO) analysis of differentially expressed genes and miRNA–mRNA co-expression networks, it was found that heat stress may be involved in the regulation of testicular atrophy and spermatogenesis disorders by affecting cell meiosis process and cell cycle. In addition, through functional enrichment analysis, co-expression regulatory network, correlation analysis and in vitro experiment, it was found that miR-143-3p may be a representative potential key regulatory factor affecting spermatogenesis under heat stress. In summary, our results enrich the understanding of miRNAs in testicular heat stress and provide a reference for the prevention and treatment of heat-stress-induced spermatogenesis disorders.https://www.mdpi.com/1422-0067/24/10/8809heat stresstesticular atrophymicroRNAsspermatogenesis disorderinfertility |
| spellingShingle | Mailin Gan Yunhong Jing Zhongwei Xie Jianfeng Ma Lei Chen Shunhua Zhang Ye Zhao Lili Niu Yan Wang Xuewei Li Li Zhu Linyuan Shen Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders International Journal of Molecular Sciences heat stress testicular atrophy microRNAs spermatogenesis disorder infertility |
| title | Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders |
| title_full | Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders |
| title_fullStr | Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders |
| title_full_unstemmed | Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders |
| title_short | Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders |
| title_sort | potential function of testicular micrornas in heat stress induced spermatogenesis disorders |
| topic | heat stress testicular atrophy microRNAs spermatogenesis disorder infertility |
| url | https://www.mdpi.com/1422-0067/24/10/8809 |
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