Nuclear dynamics in the homothallic ascomycete Sordaria fimicola
Abstract Background The coprophilous ascomycete Sordaria fimicola usually reproduces sexually. Sexual differentiation in S. fimicola is accompanied by cellular and morphological changes, followed by multicellular tissue development to complete the sexual cycle. Although the morphological features of...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-07-01
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| Series: | Botanical Studies |
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| Online Access: | http://link.springer.com/article/10.1186/s40529-018-0233-y |
| _version_ | 1811221126532038656 |
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| author | Kulsumpun Krobanan Wei-Chiang Shen |
| author_facet | Kulsumpun Krobanan Wei-Chiang Shen |
| author_sort | Kulsumpun Krobanan |
| collection | DOAJ |
| description | Abstract Background The coprophilous ascomycete Sordaria fimicola usually reproduces sexually. Sexual differentiation in S. fimicola is accompanied by cellular and morphological changes, followed by multicellular tissue development to complete the sexual cycle. Although the morphological features of the sexual reproductive structure in S. fimicola have been well characterized, little is known about the nuclear dynamics and organization during these processes. Therefore, in this study, we successfully developed an Agrobacterium-mediated protoplast transformation protocol and generated histone H2B-mCherry-labeled S. fimicola strains. The life cycle of S. fimicola begins with germination of the ascospore and ends with the formation and discharge of new ascospores from the mature black sexual fruiting bodies, the so-called perithecia. The nuclear dynamics of the fluorescently labeled strains were examined during ascospore germination, hyphal elongation, and hyphal fusion using fluorescent microscopy. Results Live imaging revealed that the nuclei in the germlings and fusion hyphae during the pre-contact interaction are located adjacent to the tip. Conclusions This is the first report of the application of a fluorescence labeling technique in S. fimicola. This application will help researchers gain a better understanding of nuclear distribution and investigate the protein–protein interaction networks during fruiting body formation for advanced molecular genetic studies in S. fimicola. |
| first_indexed | 2024-04-12T07:53:54Z |
| format | Article |
| id | doaj.art-eed393f090af47f88102f401fb60c5e0 |
| institution | Directory Open Access Journal |
| issn | 1999-3110 |
| language | English |
| last_indexed | 2024-04-12T07:53:54Z |
| publishDate | 2018-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Botanical Studies |
| spelling | doaj.art-eed393f090af47f88102f401fb60c5e02022-12-22T03:41:33ZengSpringerOpenBotanical Studies1999-31102018-07-015911710.1186/s40529-018-0233-yNuclear dynamics in the homothallic ascomycete Sordaria fimicolaKulsumpun Krobanan0Wei-Chiang Shen1Department of Plant Pathology and Microbiology, National Taiwan UniversityDepartment of Plant Pathology and Microbiology, National Taiwan UniversityAbstract Background The coprophilous ascomycete Sordaria fimicola usually reproduces sexually. Sexual differentiation in S. fimicola is accompanied by cellular and morphological changes, followed by multicellular tissue development to complete the sexual cycle. Although the morphological features of the sexual reproductive structure in S. fimicola have been well characterized, little is known about the nuclear dynamics and organization during these processes. Therefore, in this study, we successfully developed an Agrobacterium-mediated protoplast transformation protocol and generated histone H2B-mCherry-labeled S. fimicola strains. The life cycle of S. fimicola begins with germination of the ascospore and ends with the formation and discharge of new ascospores from the mature black sexual fruiting bodies, the so-called perithecia. The nuclear dynamics of the fluorescently labeled strains were examined during ascospore germination, hyphal elongation, and hyphal fusion using fluorescent microscopy. Results Live imaging revealed that the nuclei in the germlings and fusion hyphae during the pre-contact interaction are located adjacent to the tip. Conclusions This is the first report of the application of a fluorescence labeling technique in S. fimicola. This application will help researchers gain a better understanding of nuclear distribution and investigate the protein–protein interaction networks during fruiting body formation for advanced molecular genetic studies in S. fimicola.http://link.springer.com/article/10.1186/s40529-018-0233-ySordaria fimicolaHistone H2B proteinmCherry |
| spellingShingle | Kulsumpun Krobanan Wei-Chiang Shen Nuclear dynamics in the homothallic ascomycete Sordaria fimicola Botanical Studies Sordaria fimicola Histone H2B protein mCherry |
| title | Nuclear dynamics in the homothallic ascomycete Sordaria fimicola |
| title_full | Nuclear dynamics in the homothallic ascomycete Sordaria fimicola |
| title_fullStr | Nuclear dynamics in the homothallic ascomycete Sordaria fimicola |
| title_full_unstemmed | Nuclear dynamics in the homothallic ascomycete Sordaria fimicola |
| title_short | Nuclear dynamics in the homothallic ascomycete Sordaria fimicola |
| title_sort | nuclear dynamics in the homothallic ascomycete sordaria fimicola |
| topic | Sordaria fimicola Histone H2B protein mCherry |
| url | http://link.springer.com/article/10.1186/s40529-018-0233-y |
| work_keys_str_mv | AT kulsumpunkrobanan nucleardynamicsinthehomothallicascomycetesordariafimicola AT weichiangshen nucleardynamicsinthehomothallicascomycetesordariafimicola |