Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids
Cellular residues after lipid extraction are the primary waste by-products of microbial polyunsaturated fatty acid production. To mitigate the discharge of cellular residues and reduce the cost of DHA and ARA production, this study examined the utilization of enzymatically hydrolyzed <i>Aurant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/2/81 |
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| author | Feng-Wei Yin Jiao Huang Ci-Tong Zhan Xiao-Long Sun Wei-Long Zheng Xi Luo Ying-Ying Zhang Long-Fei Yin Yong-Qian Fu |
| author_facet | Feng-Wei Yin Jiao Huang Ci-Tong Zhan Xiao-Long Sun Wei-Long Zheng Xi Luo Ying-Ying Zhang Long-Fei Yin Yong-Qian Fu |
| author_sort | Feng-Wei Yin |
| collection | DOAJ |
| description | Cellular residues after lipid extraction are the primary waste by-products of microbial polyunsaturated fatty acid production. To mitigate the discharge of cellular residues and reduce the cost of DHA and ARA production, this study examined the utilization of enzymatically hydrolyzed <i>Aurantiochytrium</i> cellular residues (ACRs) and <i>Mortierella alpina</i> cellular residues (MCRs) as nitrogen sources. Results demonstrated that ACRs and MCRs could partially substitute yeast extract (YE) without adverse effects on DHA and ARA fermentation. Moreover, the implementation of a new fermentation medium incorporating ACRs, MCRs, and YE as mixed nitrogen sources resulted in DHA and ARA yields of 17.78 and 5.77 g/L, respectively. These values represented increases of 10.37% and 9.28% compared to traditional cultural methods, while simultaneously reducing the usage of YE by 80% and 60%, respectively. Therefore, the strategy of recycling waste cellular residues presents a novel approach for reducing the costs and environmental impact associated with microbial fermentations. |
| first_indexed | 2024-03-07T22:34:05Z |
| format | Article |
| id | doaj.art-eed61fd53bba4fc5b7310566b376f85e |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-07T22:34:05Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-eed61fd53bba4fc5b7310566b376f85e2024-02-23T15:15:53ZengMDPI AGFermentation2311-56372024-01-011028110.3390/fermentation10020081Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty AcidsFeng-Wei Yin0Jiao Huang1Ci-Tong Zhan2Xiao-Long Sun3Wei-Long Zheng4Xi Luo5Ying-Ying Zhang6Long-Fei Yin7Yong-Qian Fu8Taizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, ChinaSchool of Pharmaceutical Sciences, Taizhou University, Taizhou 318000, ChinaSchool of Pharmaceutical Sciences, Taizhou University, Taizhou 318000, ChinaTaizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, ChinaTaizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, ChinaTaizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, ChinaTaizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, ChinaTaizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, ChinaTaizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, ChinaCellular residues after lipid extraction are the primary waste by-products of microbial polyunsaturated fatty acid production. To mitigate the discharge of cellular residues and reduce the cost of DHA and ARA production, this study examined the utilization of enzymatically hydrolyzed <i>Aurantiochytrium</i> cellular residues (ACRs) and <i>Mortierella alpina</i> cellular residues (MCRs) as nitrogen sources. Results demonstrated that ACRs and MCRs could partially substitute yeast extract (YE) without adverse effects on DHA and ARA fermentation. Moreover, the implementation of a new fermentation medium incorporating ACRs, MCRs, and YE as mixed nitrogen sources resulted in DHA and ARA yields of 17.78 and 5.77 g/L, respectively. These values represented increases of 10.37% and 9.28% compared to traditional cultural methods, while simultaneously reducing the usage of YE by 80% and 60%, respectively. Therefore, the strategy of recycling waste cellular residues presents a novel approach for reducing the costs and environmental impact associated with microbial fermentations.https://www.mdpi.com/2311-5637/10/2/81cellular residuesfermentationpolyunsaturated fatty acid<i>Aurantiochytrium</i> sp.<i>Mortierella alpina</i> |
| spellingShingle | Feng-Wei Yin Jiao Huang Ci-Tong Zhan Xiao-Long Sun Wei-Long Zheng Xi Luo Ying-Ying Zhang Long-Fei Yin Yong-Qian Fu Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids Fermentation cellular residues fermentation polyunsaturated fatty acid <i>Aurantiochytrium</i> sp. <i>Mortierella alpina</i> |
| title | Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids |
| title_full | Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids |
| title_fullStr | Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids |
| title_full_unstemmed | Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids |
| title_short | Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids |
| title_sort | recycling fermentation strategy for waste cellular residues in the production of polyunsaturated fatty acids |
| topic | cellular residues fermentation polyunsaturated fatty acid <i>Aurantiochytrium</i> sp. <i>Mortierella alpina</i> |
| url | https://www.mdpi.com/2311-5637/10/2/81 |
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