Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial Strategy
Human T-cell leukemia virus type 1 (HTLV-1), a retrovirus, causes adult T-cell leukemia-lymphoma, HTLV-1 associated myelopathy/tropical spastic paraparesis, and HTLV-1 uveitis. Currently, no antiretroviral therapies or vaccines are available for HTLV-1 infection. This study aimed to develop an antib...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/10/2153 |
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| author | Yasuyoshi Hatayama Yutaro Yamaoka Takeshi Morita Sundararaj Stanleyraj Jeremiah Kei Miyakawa Mayuko Nishi Yayoi Kimura Makoto Mitsunaga Tadayuki Iwase Hirokazu Kimura Naoki Yamamoto Akifumi Takaori-Kondo Hideki Hasegawa Akihide Ryo |
| author_facet | Yasuyoshi Hatayama Yutaro Yamaoka Takeshi Morita Sundararaj Stanleyraj Jeremiah Kei Miyakawa Mayuko Nishi Yayoi Kimura Makoto Mitsunaga Tadayuki Iwase Hirokazu Kimura Naoki Yamamoto Akifumi Takaori-Kondo Hideki Hasegawa Akihide Ryo |
| author_sort | Yasuyoshi Hatayama |
| collection | DOAJ |
| description | Human T-cell leukemia virus type 1 (HTLV-1), a retrovirus, causes adult T-cell leukemia-lymphoma, HTLV-1 associated myelopathy/tropical spastic paraparesis, and HTLV-1 uveitis. Currently, no antiretroviral therapies or vaccines are available for HTLV-1 infection. This study aimed to develop an antibody against the HTLV-1 envelope protein (Env) and apply it to a near-infrared photoimmuno-antimicrobial strategy (NIR-PIAS) to eliminate HTLV-1 infected cells. We established mouse monoclonal antibodies (mAbs) against HTLV-1 Env by immunization with a complex of liposome and the recombinant protein. Detailed epitope mapping revealed that one of the mAbs bound to the proline-rich region of gp46 and exhibited no obvious neutralizing activity to inhibit viral infection. Instead, the mAb was rarely internalized intracellularly and remained on the cell surface of HTLV-1-infected cells. The antibody conjugated to the photosensitive dye IRDye700Dx recognized HTLV-1 infected cells and killed them following NIR irradiation. These results suggest that the novel mAb and NIR-PIAS could be developed as a new targeted therapeutic tool against HTLV-1 infected cells. |
| first_indexed | 2024-03-09T19:24:27Z |
| format | Article |
| id | doaj.art-eed65c7af9ef4378ad9b368b590f0c2e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T19:24:27Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-eed65c7af9ef4378ad9b368b590f0c2e2023-11-24T03:08:22ZengMDPI AGViruses1999-49152022-09-011410215310.3390/v14102153Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial StrategyYasuyoshi Hatayama0Yutaro Yamaoka1Takeshi Morita2Sundararaj Stanleyraj Jeremiah3Kei Miyakawa4Mayuko Nishi5Yayoi Kimura6Makoto Mitsunaga7Tadayuki Iwase8Hirokazu Kimura9Naoki Yamamoto10Akifumi Takaori-Kondo11Hideki Hasegawa12Akihide Ryo13Department of Microbiology, Yokohama City University School of Medicine, Yokohama 236-0004, JapanDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama 236-0004, JapanDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama 236-0004, JapanDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama 236-0004, JapanDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama 236-0004, JapanDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama 236-0004, JapanAdvanced Medical Research Center, Yokohama City University, Yokohama 236-0004, JapanDivision of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Minato 105-8461, JapanResearch Center for Medical Sciences, The Jikei University School of Medicine, 3-25-8, Minato 105-8461, JapanDepartment of Health Science, Gunma Paz University Graduate School, Takasaki 370-0006, JapanGenome Medical Sciences Project, National Center for Global Health and Medicine, Ichikawa 272-8516, JapanDepartment of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, JapanCenter for Influenza and Respiratory Virus Research, National Institute of Infectious Diseases, Musashimurayama 208-0011, JapanDepartment of Microbiology, Yokohama City University School of Medicine, Yokohama 236-0004, JapanHuman T-cell leukemia virus type 1 (HTLV-1), a retrovirus, causes adult T-cell leukemia-lymphoma, HTLV-1 associated myelopathy/tropical spastic paraparesis, and HTLV-1 uveitis. Currently, no antiretroviral therapies or vaccines are available for HTLV-1 infection. This study aimed to develop an antibody against the HTLV-1 envelope protein (Env) and apply it to a near-infrared photoimmuno-antimicrobial strategy (NIR-PIAS) to eliminate HTLV-1 infected cells. We established mouse monoclonal antibodies (mAbs) against HTLV-1 Env by immunization with a complex of liposome and the recombinant protein. Detailed epitope mapping revealed that one of the mAbs bound to the proline-rich region of gp46 and exhibited no obvious neutralizing activity to inhibit viral infection. Instead, the mAb was rarely internalized intracellularly and remained on the cell surface of HTLV-1-infected cells. The antibody conjugated to the photosensitive dye IRDye700Dx recognized HTLV-1 infected cells and killed them following NIR irradiation. These results suggest that the novel mAb and NIR-PIAS could be developed as a new targeted therapeutic tool against HTLV-1 infected cells.https://www.mdpi.com/1999-4915/14/10/2153HTLV-1monoclonal antibodynear infrared photoimmuno-antimicrobial strategyIR700 |
| spellingShingle | Yasuyoshi Hatayama Yutaro Yamaoka Takeshi Morita Sundararaj Stanleyraj Jeremiah Kei Miyakawa Mayuko Nishi Yayoi Kimura Makoto Mitsunaga Tadayuki Iwase Hirokazu Kimura Naoki Yamamoto Akifumi Takaori-Kondo Hideki Hasegawa Akihide Ryo Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial Strategy Viruses HTLV-1 monoclonal antibody near infrared photoimmuno-antimicrobial strategy IR700 |
| title | Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial Strategy |
| title_full | Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial Strategy |
| title_fullStr | Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial Strategy |
| title_full_unstemmed | Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial Strategy |
| title_short | Development of a Monoclonal Antibody Targeting HTLV-1 Envelope gp46 Glycoprotein and Its Application to Near-Infrared Photoimmuno-Antimicrobial Strategy |
| title_sort | development of a monoclonal antibody targeting htlv 1 envelope gp46 glycoprotein and its application to near infrared photoimmuno antimicrobial strategy |
| topic | HTLV-1 monoclonal antibody near infrared photoimmuno-antimicrobial strategy IR700 |
| url | https://www.mdpi.com/1999-4915/14/10/2153 |
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