A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold
The desirable properties of the sophisticated fluorescent pH probe are ratiometric detection properties and a wide detection range. In this study, three types of fluorophores with different fluorescence properties were assembled on a DNA origami nanostructure. DNA nanostructure has the advantage of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Chemistry |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2624-8549/5/3/125 |
| _version_ | 1797580844947210240 |
|---|---|
| author | Eiji Nakata Khongorzul Gerelbaatar Mashal Asif Hiroaki Konishi Yuya Shibano Peng Lin Takashi Morii |
| author_facet | Eiji Nakata Khongorzul Gerelbaatar Mashal Asif Hiroaki Konishi Yuya Shibano Peng Lin Takashi Morii |
| author_sort | Eiji Nakata |
| collection | DOAJ |
| description | The desirable properties of the sophisticated fluorescent pH probe are ratiometric detection properties and a wide detection range. In this study, three types of fluorophores with different fluorescence properties were assembled on a DNA origami nanostructure. DNA nanostructure has the advantage of being a scaffold that can assemble different types of fluorophores with control over their number and position. The defined number of three different fluorophores, i.e., pH-sensitive fluorescein (CF) and Oregon Green (OG), and pH-insensitive tetramethylrhodamine (CR), assembled on the DNA scaffold provided a ratiometric fluorescent pH probe with a wide pH detection range that could cover the variation of intracellular pH. |
| first_indexed | 2024-03-10T22:55:46Z |
| format | Article |
| id | doaj.art-eed6f00e10d847e49239e9b5fec00ec6 |
| institution | Directory Open Access Journal |
| issn | 2624-8549 |
| language | English |
| last_indexed | 2024-03-10T22:55:46Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemistry |
| spelling | doaj.art-eed6f00e10d847e49239e9b5fec00ec62023-11-19T10:01:33ZengMDPI AGChemistry2624-85492023-08-01531832184210.3390/chemistry5030125A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA ScaffoldEiji Nakata0Khongorzul Gerelbaatar1Mashal Asif2Hiroaki Konishi3Yuya Shibano4Peng Lin5Takashi Morii6Institute of Advanced Energy, Kyoto University, Kyoto 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Kyoto 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Kyoto 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Kyoto 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Kyoto 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Kyoto 611-0011, JapanInstitute of Advanced Energy, Kyoto University, Kyoto 611-0011, JapanThe desirable properties of the sophisticated fluorescent pH probe are ratiometric detection properties and a wide detection range. In this study, three types of fluorophores with different fluorescence properties were assembled on a DNA origami nanostructure. DNA nanostructure has the advantage of being a scaffold that can assemble different types of fluorophores with control over their number and position. The defined number of three different fluorophores, i.e., pH-sensitive fluorescein (CF) and Oregon Green (OG), and pH-insensitive tetramethylrhodamine (CR), assembled on the DNA scaffold provided a ratiometric fluorescent pH probe with a wide pH detection range that could cover the variation of intracellular pH.https://www.mdpi.com/2624-8549/5/3/125ratiometric fluorescent probepH-sensitive fluorophorepH detectionDNA nanostructureDNA origami scaffold |
| spellingShingle | Eiji Nakata Khongorzul Gerelbaatar Mashal Asif Hiroaki Konishi Yuya Shibano Peng Lin Takashi Morii A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold Chemistry ratiometric fluorescent probe pH-sensitive fluorophore pH detection DNA nanostructure DNA origami scaffold |
| title | A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold |
| title_full | A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold |
| title_fullStr | A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold |
| title_full_unstemmed | A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold |
| title_short | A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold |
| title_sort | ratiometric fluorescent probe for ph measurement over a wide range composed of three types of fluorophores assembled on a dna scaffold |
| topic | ratiometric fluorescent probe pH-sensitive fluorophore pH detection DNA nanostructure DNA origami scaffold |
| url | https://www.mdpi.com/2624-8549/5/3/125 |
| work_keys_str_mv | AT eijinakata aratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT khongorzulgerelbaatar aratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT mashalasif aratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT hiroakikonishi aratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT yuyashibano aratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT penglin aratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT takashimorii aratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT eijinakata ratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT khongorzulgerelbaatar ratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT mashalasif ratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT hiroakikonishi ratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT yuyashibano ratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT penglin ratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold AT takashimorii ratiometricfluorescentprobeforphmeasurementoverawiderangecomposedofthreetypesoffluorophoresassembledonadnascaffold |