AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4
The objective of this study was to identify genes associated with the biodegradation of phenol by Acinetobacter sp. strain DF4 through the use of differential display (DD) methodology. The bacteria were grown in YEPG medium, and total RNA was extracted and analyzed using labeled primers to detect ge...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X23015202 |
| _version_ | 1827266228524154880 |
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| author | Desouky Abd-El-Haleem |
| author_facet | Desouky Abd-El-Haleem |
| author_sort | Desouky Abd-El-Haleem |
| collection | DOAJ |
| description | The objective of this study was to identify genes associated with the biodegradation of phenol by Acinetobacter sp. strain DF4 through the use of differential display (DD) methodology. The bacteria were grown in YEPG medium, and total RNA was extracted and analyzed using labeled primers to detect gene expression differences. Three distinctively expressed cDNA bands (ph1, ph2, and ph3) were identified, cloned, and sequenced. DNA analysis involved searching for open reading frames (ORFs), verifying results with the NCBI database, predicting promoter regions, and constructing phylogenetic trees using bioinformatics tools. The ph1 gene displayed a 97% identity with the AraC transcriptional regulator, suggesting its potential role in regulating the ortho-catabolic pathway of phenol. The ph2 gene showed a 98% identity with aspartate semialdehyde dehydrogenase, which is involved in phenol degradation. The ph3 gene had a 93% identity with acetyltransferase. Essential transcription factors, such as TATA, GTGTGT, CACA, and CTTTT, were detected, and the three genes promoter regions were predicted. This study successfully identified functional genes involved in the metabolism of cyclic chemicals, particularly phenol, using the DD technique. These findings provide insights into the biodegradation pathways of phenol by Acinetobacter sp. Strain DF4 and may contribute to the development of more efficient bioremediation strategies for phenol-contaminated environments. |
| first_indexed | 2024-04-24T23:18:58Z |
| format | Article |
| id | doaj.art-eed8d649f6294df2bee5cc64e01facef |
| institution | Directory Open Access Journal |
| issn | 1687-157X |
| language | English |
| last_indexed | 2025-03-22T04:14:31Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
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| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-eed8d649f6294df2bee5cc64e01facef2024-04-28T08:30:56ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2024-03-01221100349AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4Desouky Abd-El-Haleem0Environmental Biotechnology Department, Genetic Engineering and Biotechnology Institute, City of Scientific Research and Technological Applications, Burgelarab, Alexandria, EgyptThe objective of this study was to identify genes associated with the biodegradation of phenol by Acinetobacter sp. strain DF4 through the use of differential display (DD) methodology. The bacteria were grown in YEPG medium, and total RNA was extracted and analyzed using labeled primers to detect gene expression differences. Three distinctively expressed cDNA bands (ph1, ph2, and ph3) were identified, cloned, and sequenced. DNA analysis involved searching for open reading frames (ORFs), verifying results with the NCBI database, predicting promoter regions, and constructing phylogenetic trees using bioinformatics tools. The ph1 gene displayed a 97% identity with the AraC transcriptional regulator, suggesting its potential role in regulating the ortho-catabolic pathway of phenol. The ph2 gene showed a 98% identity with aspartate semialdehyde dehydrogenase, which is involved in phenol degradation. The ph3 gene had a 93% identity with acetyltransferase. Essential transcription factors, such as TATA, GTGTGT, CACA, and CTTTT, were detected, and the three genes promoter regions were predicted. This study successfully identified functional genes involved in the metabolism of cyclic chemicals, particularly phenol, using the DD technique. These findings provide insights into the biodegradation pathways of phenol by Acinetobacter sp. Strain DF4 and may contribute to the development of more efficient bioremediation strategies for phenol-contaminated environments.http://www.sciencedirect.com/science/article/pii/S1687157X23015202Putative genesDifferential Display- Random primersPhenol catabolic pathwayAraC transcriptional regulatorDehydrogenaseAcetyltransferase |
| spellingShingle | Desouky Abd-El-Haleem AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4 Journal of Genetic Engineering and Biotechnology Putative genes Differential Display- Random primers Phenol catabolic pathway AraC transcriptional regulator Dehydrogenase Acetyltransferase |
| title | AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4 |
| title_full | AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4 |
| title_fullStr | AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4 |
| title_full_unstemmed | AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4 |
| title_short | AraC transcriptional regulator, aspartate semialdehyde dehydrogenase and acyltransferase: Three putative genes in phenol catabolic pathway of Acinetobacter sp. Strain DF4 |
| title_sort | arac transcriptional regulator aspartate semialdehyde dehydrogenase and acyltransferase three putative genes in phenol catabolic pathway of acinetobacter sp strain df4 |
| topic | Putative genes Differential Display- Random primers Phenol catabolic pathway AraC transcriptional regulator Dehydrogenase Acetyltransferase |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X23015202 |
| work_keys_str_mv | AT desoukyabdelhaleem aractranscriptionalregulatoraspartatesemialdehydedehydrogenaseandacyltransferasethreeputativegenesinphenolcatabolicpathwayofacinetobacterspstraindf4 |