Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction

This study aims to compare the suitability of three extraction techniques (cold pressing, Soxhlet and supercritical fluid extraction (SFE)) to isolate oil from cherry seeds. Oils were examined in terms of extraction yield, fatty acids profile, tocopherols yield and antioxidant activity. Additionally...

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Main Authors: Ivana Dimić, Branimir Pavlić, Slađana Rakita, Aleksandra Cvetanović Kljakić, Zoran Zeković, Nemanja Teslić
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/12/1/11
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author Ivana Dimić
Branimir Pavlić
Slađana Rakita
Aleksandra Cvetanović Kljakić
Zoran Zeković
Nemanja Teslić
author_facet Ivana Dimić
Branimir Pavlić
Slađana Rakita
Aleksandra Cvetanović Kljakić
Zoran Zeković
Nemanja Teslić
author_sort Ivana Dimić
collection DOAJ
description This study aims to compare the suitability of three extraction techniques (cold pressing, Soxhlet and supercritical fluid extraction (SFE)) to isolate oil from cherry seeds. Oils were examined in terms of extraction yield, fatty acids profile, tocopherols yield and antioxidant activity. Additionally, influence of SFE parameters was evaluated using one-factor-at-a-time design with pressure (200–350 bar), temperature (40–70 °C), flow rate (0.2–0.4 kg/h) and particle size (<800 µm and >800 µm). Oil yields ranged from 2.50% to 13.02%, whereas the highest yield was achieved with SFE. Samples were rich in polyunsaturated fatty acids, regardless of the applied extraction technique. The main fatty acids were linoleic (46.32–47.29%), oleic (40.89–41.65%), palmitic (6.56–8.00%) and stearic (2.21–2.30%) acid. Total tocopherols yield was between 16.63 mg/100 g oil and 60.61 mg/100 g oil, and highest yield was achieved with SFE. Among the tocopherols, γ-tocopherol was the most abundant, followed by α-, δ- and β-tocopherol. Antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic) cation (ABTS) assays, and the results indicated that SFE extracts exhibited better or comparable antioxidant potential compared to traditional techniques. The comparison between modern and conventional extractions for oil recovery demonstrates pros and cons for the possibility of industrial application.
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spelling doaj.art-eed9957712d642ba8623656cf35ab81f2023-11-16T15:21:08ZengMDPI AGFoods2304-81582022-12-011211110.3390/foods12010011Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid ExtractionIvana Dimić0Branimir Pavlić1Slađana Rakita2Aleksandra Cvetanović Kljakić3Zoran Zeković4Nemanja Teslić5Faculty of Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, SerbiaFaculty of Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, SerbiaInstitute of Food Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, SerbiaFaculty of Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, SerbiaFaculty of Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, SerbiaInstitute of Food Technology, University of Novi Sad, Blvd. cara Lazara 1, 21000 Novi Sad, SerbiaThis study aims to compare the suitability of three extraction techniques (cold pressing, Soxhlet and supercritical fluid extraction (SFE)) to isolate oil from cherry seeds. Oils were examined in terms of extraction yield, fatty acids profile, tocopherols yield and antioxidant activity. Additionally, influence of SFE parameters was evaluated using one-factor-at-a-time design with pressure (200–350 bar), temperature (40–70 °C), flow rate (0.2–0.4 kg/h) and particle size (<800 µm and >800 µm). Oil yields ranged from 2.50% to 13.02%, whereas the highest yield was achieved with SFE. Samples were rich in polyunsaturated fatty acids, regardless of the applied extraction technique. The main fatty acids were linoleic (46.32–47.29%), oleic (40.89–41.65%), palmitic (6.56–8.00%) and stearic (2.21–2.30%) acid. Total tocopherols yield was between 16.63 mg/100 g oil and 60.61 mg/100 g oil, and highest yield was achieved with SFE. Among the tocopherols, γ-tocopherol was the most abundant, followed by α-, δ- and β-tocopherol. Antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic) cation (ABTS) assays, and the results indicated that SFE extracts exhibited better or comparable antioxidant potential compared to traditional techniques. The comparison between modern and conventional extractions for oil recovery demonstrates pros and cons for the possibility of industrial application.https://www.mdpi.com/2304-8158/12/1/11sour cherry seed oil<i>Prunus cerasus</i> L.extraction techniqueschemical compositionantioxidant activity
spellingShingle Ivana Dimić
Branimir Pavlić
Slađana Rakita
Aleksandra Cvetanović Kljakić
Zoran Zeković
Nemanja Teslić
Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction
Foods
sour cherry seed oil
<i>Prunus cerasus</i> L.
extraction techniques
chemical composition
antioxidant activity
title Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction
title_full Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction
title_fullStr Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction
title_full_unstemmed Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction
title_short Isolation of Cherry Seed Oil Using Conventional Techniques and Supercritical Fluid Extraction
title_sort isolation of cherry seed oil using conventional techniques and supercritical fluid extraction
topic sour cherry seed oil
<i>Prunus cerasus</i> L.
extraction techniques
chemical composition
antioxidant activity
url https://www.mdpi.com/2304-8158/12/1/11
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AT aleksandracvetanovickljakic isolationofcherryseedoilusingconventionaltechniquesandsupercriticalfluidextraction
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