Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China
Abstract Background Wenzhou virus (WENV), a newly discovered mammarenavirus in rodents, is associated with fever and respiratory symptoms in humans. This study was aimed to detect and characterize the emerging virus in rodents in Guangzhou, China. Results A total of 100 small mammals, including 70 R...
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-021-03009-2 |
| _version_ | 1818728945470668800 |
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| author | Nina Wang Lichao Yang Guohui Li Xu Zhang Jianwei Shao Jun Ma Shouyi Chen Quan Liu |
| author_facet | Nina Wang Lichao Yang Guohui Li Xu Zhang Jianwei Shao Jun Ma Shouyi Chen Quan Liu |
| author_sort | Nina Wang |
| collection | DOAJ |
| description | Abstract Background Wenzhou virus (WENV), a newly discovered mammarenavirus in rodents, is associated with fever and respiratory symptoms in humans. This study was aimed to detect and characterize the emerging virus in rodents in Guangzhou, China. Results A total of 100 small mammals, including 70 Rattus norvegicus, 22 Suncus murinus, 4 Bandicota indica, 3 Rattus flavipectus, and 1 Rattus losea, were captured in Guangzhou, and their brain tissues were collected and pooled for metagenomic analysis, which generated several contigs targeting the genome of WENV. Two R. norvegicus (2.9%) were further confirmed to be infected with WENV by RT-PCR. The complete genome (RnGZ37-2018 and RnGZ40-2018) shared 85.1–88.9% nt and 83.2–96.3% aa sequence identities to the Cambodian strains that have been shown to be associated with human disease. Phylogenetic analysis showed that all identified WENV could be grouped into four different lineages, and the two Guangzhou strains formed an independent clade. We also analyzed the potential recombinant events occurring in WENV strains. Conclusions Our study showed a high genetic diversity of WENV strains in China, emphasizing the relevance of surveillance of this emerging mammarenavirus in both natural reservoirs and humans. |
| first_indexed | 2024-12-17T22:38:03Z |
| format | Article |
| id | doaj.art-eed9dbaf394747a4970dfccd7421e024 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-17T22:38:03Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-eed9dbaf394747a4970dfccd7421e0242022-12-21T21:30:03ZengBMCBMC Veterinary Research1746-61482021-09-011711810.1186/s12917-021-03009-2Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, ChinaNina Wang0Lichao Yang1Guohui Li2Xu Zhang3Jianwei Shao4Jun Ma5Shouyi Chen6Quan Liu7School of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversitySchool of Life Sciences and Engineering, Foshan UniversityGuangzhou Center for Disease Control and PreventionSchool of Life Sciences and Engineering, Foshan UniversityAbstract Background Wenzhou virus (WENV), a newly discovered mammarenavirus in rodents, is associated with fever and respiratory symptoms in humans. This study was aimed to detect and characterize the emerging virus in rodents in Guangzhou, China. Results A total of 100 small mammals, including 70 Rattus norvegicus, 22 Suncus murinus, 4 Bandicota indica, 3 Rattus flavipectus, and 1 Rattus losea, were captured in Guangzhou, and their brain tissues were collected and pooled for metagenomic analysis, which generated several contigs targeting the genome of WENV. Two R. norvegicus (2.9%) were further confirmed to be infected with WENV by RT-PCR. The complete genome (RnGZ37-2018 and RnGZ40-2018) shared 85.1–88.9% nt and 83.2–96.3% aa sequence identities to the Cambodian strains that have been shown to be associated with human disease. Phylogenetic analysis showed that all identified WENV could be grouped into four different lineages, and the two Guangzhou strains formed an independent clade. We also analyzed the potential recombinant events occurring in WENV strains. Conclusions Our study showed a high genetic diversity of WENV strains in China, emphasizing the relevance of surveillance of this emerging mammarenavirus in both natural reservoirs and humans.https://doi.org/10.1186/s12917-021-03009-2Wenzhou virusmammarenavirusrodentsphylogenetic analysisGuangzhouChina |
| spellingShingle | Nina Wang Lichao Yang Guohui Li Xu Zhang Jianwei Shao Jun Ma Shouyi Chen Quan Liu Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China BMC Veterinary Research Wenzhou virus mammarenavirus rodents phylogenetic analysis Guangzhou China |
| title | Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China |
| title_full | Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China |
| title_fullStr | Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China |
| title_full_unstemmed | Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China |
| title_short | Molecular detection and genetic characterization of Wenzhou virus in rodents in Guangzhou, China |
| title_sort | molecular detection and genetic characterization of wenzhou virus in rodents in guangzhou china |
| topic | Wenzhou virus mammarenavirus rodents phylogenetic analysis Guangzhou China |
| url | https://doi.org/10.1186/s12917-021-03009-2 |
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