Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue
Production of fumaric acid from xylo-oligosaccharides manufacturing waste residue (XOR) by Rhizopus oryzae CICC 40351 was investigated in a simultaneous saccharification and fermentation (SSF) process. The fermentation conditions for SSF were optimized by an orthogonal design method to maximize the...
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North Carolina State University
2016-08-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_4_8831_Li_Production_Fumaric_Acid_Rhizopus_Oryzae |
| _version_ | 1811276980759298048 |
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| author | Xin Li Ximei Gu Chenhuan Lai Jia Ouyang Qiang Yong |
| author_facet | Xin Li Ximei Gu Chenhuan Lai Jia Ouyang Qiang Yong |
| author_sort | Xin Li |
| collection | DOAJ |
| description | Production of fumaric acid from xylo-oligosaccharides manufacturing waste residue (XOR) by Rhizopus oryzae CICC 40351 was investigated in a simultaneous saccharification and fermentation (SSF) process. The fermentation conditions for SSF were optimized by an orthogonal design method to maximize the fumaric acid concentration. The highest fumaric acid concentration (12.54 g/L) was reached with a substrate loading of 5% (w/v) XOR in the SSF process at 38 °C. The fumaric acid concentration of the SSF process was 1.8 times greater than that of the separate hydrolysis and fermentation (SHF) process under the same conditions. In addition, the SSF process yielded 0.34 g/g of glucose, whereas the SHF process yielded only 0.20 g/g of glucose. The results indicated that the SSF process notably improved the production of fumaric acid from lignocellulose by R. oryzae. |
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| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-04-13T00:07:30Z |
| publishDate | 2016-08-01 |
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| series | BioResources |
| spelling | doaj.art-eee3862f21424e53b1a6c7e64b9655812022-12-22T03:11:11ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-08-011148831884310.15376/biores.11.4.8831-8843Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste ResidueXin Li0Ximei Gu1Chenhuan Lai2Jia Ouyang3Qiang Yong4Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; ChinaCollege of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; ChinaJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; ChinaJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; College of; ChinaJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, China; College of; ChinaProduction of fumaric acid from xylo-oligosaccharides manufacturing waste residue (XOR) by Rhizopus oryzae CICC 40351 was investigated in a simultaneous saccharification and fermentation (SSF) process. The fermentation conditions for SSF were optimized by an orthogonal design method to maximize the fumaric acid concentration. The highest fumaric acid concentration (12.54 g/L) was reached with a substrate loading of 5% (w/v) XOR in the SSF process at 38 °C. The fumaric acid concentration of the SSF process was 1.8 times greater than that of the separate hydrolysis and fermentation (SHF) process under the same conditions. In addition, the SSF process yielded 0.34 g/g of glucose, whereas the SHF process yielded only 0.20 g/g of glucose. The results indicated that the SSF process notably improved the production of fumaric acid from lignocellulose by R. oryzae.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_4_8831_Li_Production_Fumaric_Acid_Rhizopus_OryzaeRhizopus oryzaeLignocelluloseFumaric acidSimultaneous saccharification and fermentation |
| spellingShingle | Xin Li Ximei Gu Chenhuan Lai Jia Ouyang Qiang Yong Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue BioResources Rhizopus oryzae Lignocellulose Fumaric acid Simultaneous saccharification and fermentation |
| title | Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue |
| title_full | Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue |
| title_fullStr | Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue |
| title_full_unstemmed | Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue |
| title_short | Production of Fumaric Acid by Rhizopus oryzae in Simultaneous Saccharification and Fermentation using Xylo-Oligosaccharides Manufacturing Waste Residue |
| title_sort | production of fumaric acid by rhizopus oryzae in simultaneous saccharification and fermentation using xylo oligosaccharides manufacturing waste residue |
| topic | Rhizopus oryzae Lignocellulose Fumaric acid Simultaneous saccharification and fermentation |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_4_8831_Li_Production_Fumaric_Acid_Rhizopus_Oryzae |
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