Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> Rhizomes
The major bioactive components of <i>Kaempferia parviflora</i> (KP) rhizomes, 3,5,7,3′,4′-pentamethoxyflavone (PMF), 5,7-dimethoxyflavone (DMF), and 5,7,4′-trimethoxyflavone (TMF), were chosen as the quantitative and qualitative markers for this plant material. In order to extract bioact...
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/4162 |
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| author | Wantanwa Krongrawa Sontaya Limmatvapirat Supachai Saibua Chutima Limmatvapirat |
| author_facet | Wantanwa Krongrawa Sontaya Limmatvapirat Supachai Saibua Chutima Limmatvapirat |
| author_sort | Wantanwa Krongrawa |
| collection | DOAJ |
| description | The major bioactive components of <i>Kaempferia parviflora</i> (KP) rhizomes, 3,5,7,3′,4′-pentamethoxyflavone (PMF), 5,7-dimethoxyflavone (DMF), and 5,7,4′-trimethoxyflavone (TMF), were chosen as the quantitative and qualitative markers for this plant material. In order to extract bioactive components (total methoxyflavones) from KP rhizomes, ultrasound-assisted extraction (UAE) was proposed as part of this study. Plackett–Burman design (PBD) and Box–Behnken design (BBD) were utilized to optimize the effects of UAE on extraction yields and total methoxyflavone contents in KP rhizomes. First, PBD was utilized to determine the effect of five independent variables on total yields and total methoxyflavone contents. The results indicated that the concentration of the extracting solvent (ethanol), the extraction time, and the ratio of solvent to solid were significant independent terms. Subsequently, BBD with three-level factorial experiments was used to optimize the crucial variables. It was discovered that the concentration of ethanol was the most influential variable on yields and total methoxyflavone contents. Optimum conditions for extraction yield were ethanol concentration (54.24% <i>v</i>/<i>v</i>), extraction time (25.25 min), and solvent-to-solid ratio (49.63 mL/g), while optimum conditions for total methoxyflavone content were ethanol concentration (95.00% <i>v</i>/<i>v</i>), extraction time (15.99 min), and solvent-to-solid ratio (50.00 mL/g). The relationship between the experimental and theoretical values was perfect, which proved that the regression models used were correct and that PBD and BBD were used to optimize the conditions in the UAE to obtain the highest yield and total methoxyflavone content in the KP rhizomes. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:00:58Z |
| publishDate | 2022-06-01 |
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| series | Molecules |
| spelling | doaj.art-eee4330eb0a04d84abcf2089a453afe12023-12-03T14:13:34ZengMDPI AGMolecules1420-30492022-06-012713416210.3390/molecules27134162Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> RhizomesWantanwa Krongrawa0Sontaya Limmatvapirat1Supachai Saibua2Chutima Limmatvapirat3Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, ThailandBangkok Lab & Cosmetic Co., Ltd., 48/1, Moo 5, Nongsaesao Road, Tambon Namphu, Amphoe Muang Ratchaburi, Ratchaburi 70000, ThailandPharmaceutical Biopolymer Group (PBiG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, ThailandThe major bioactive components of <i>Kaempferia parviflora</i> (KP) rhizomes, 3,5,7,3′,4′-pentamethoxyflavone (PMF), 5,7-dimethoxyflavone (DMF), and 5,7,4′-trimethoxyflavone (TMF), were chosen as the quantitative and qualitative markers for this plant material. In order to extract bioactive components (total methoxyflavones) from KP rhizomes, ultrasound-assisted extraction (UAE) was proposed as part of this study. Plackett–Burman design (PBD) and Box–Behnken design (BBD) were utilized to optimize the effects of UAE on extraction yields and total methoxyflavone contents in KP rhizomes. First, PBD was utilized to determine the effect of five independent variables on total yields and total methoxyflavone contents. The results indicated that the concentration of the extracting solvent (ethanol), the extraction time, and the ratio of solvent to solid were significant independent terms. Subsequently, BBD with three-level factorial experiments was used to optimize the crucial variables. It was discovered that the concentration of ethanol was the most influential variable on yields and total methoxyflavone contents. Optimum conditions for extraction yield were ethanol concentration (54.24% <i>v</i>/<i>v</i>), extraction time (25.25 min), and solvent-to-solid ratio (49.63 mL/g), while optimum conditions for total methoxyflavone content were ethanol concentration (95.00% <i>v</i>/<i>v</i>), extraction time (15.99 min), and solvent-to-solid ratio (50.00 mL/g). The relationship between the experimental and theoretical values was perfect, which proved that the regression models used were correct and that PBD and BBD were used to optimize the conditions in the UAE to obtain the highest yield and total methoxyflavone content in the KP rhizomes.https://www.mdpi.com/1420-3049/27/13/4162<i>Kaempferia parviflora</i>ultrasound-assisted extractionmethoxyflavonePlackett–Burman designBox–Behnken design |
| spellingShingle | Wantanwa Krongrawa Sontaya Limmatvapirat Supachai Saibua Chutima Limmatvapirat Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> Rhizomes Molecules <i>Kaempferia parviflora</i> ultrasound-assisted extraction methoxyflavone Plackett–Burman design Box–Behnken design |
| title | Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> Rhizomes |
| title_full | Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> Rhizomes |
| title_fullStr | Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> Rhizomes |
| title_full_unstemmed | Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> Rhizomes |
| title_short | Optimization of Ultrasound-Assisted Extraction of Yields and Total Methoxyflavone Contents from <i>Kaempferia parviflora</i> Rhizomes |
| title_sort | optimization of ultrasound assisted extraction of yields and total methoxyflavone contents from i kaempferia parviflora i rhizomes |
| topic | <i>Kaempferia parviflora</i> ultrasound-assisted extraction methoxyflavone Plackett–Burman design Box–Behnken design |
| url | https://www.mdpi.com/1420-3049/27/13/4162 |
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