Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAE
Objective: Studies have shown that Wuzi Yanzong Pill (WYP) can be used to treat neurological diseases, but its mechanisms for multiple sclerosis (MS) remain unclear. This study aims to determine the effect of WYP on MS in an animal model of experimental autoimmune encephalomyelitis (EAE), and explor...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023078295 |
| _version_ | 1797646551086006272 |
|---|---|
| author | Yan-Rong Li Ruo-Nan Zhang Rui-Rui Sun Yan-Yan Li Bo Zhang Xiao-Ming Jin Hai-Fei Zhang Bao-Guo Xiao Cun-Gen Ma Hui-Jie Fan Zhi Chai |
| author_facet | Yan-Rong Li Ruo-Nan Zhang Rui-Rui Sun Yan-Yan Li Bo Zhang Xiao-Ming Jin Hai-Fei Zhang Bao-Guo Xiao Cun-Gen Ma Hui-Jie Fan Zhi Chai |
| author_sort | Yan-Rong Li |
| collection | DOAJ |
| description | Objective: Studies have shown that Wuzi Yanzong Pill (WYP) can be used to treat neurological diseases, but its mechanisms for multiple sclerosis (MS) remain unclear. This study aims to determine the effect of WYP on MS in an animal model of experimental autoimmune encephalomyelitis (EAE), and explore its mechanism. To provide theoretical basis for the clinical treatment of MS with WYP. Methods: C57BL/6 female mice were randomly divided into Blank control, EAE control, low dose WYP, medium dose WYP, and high dose WYP groups. One week before model generation, the mice were gavaged with saline (50 mL/kg/d) in Blank control and EAE control groups. The treatment groups was gavaged with different doses of WYP solution (4, 8, or 16 g/kg/d respectively) Clinical scores were recorded daily. Sample collection was conducted on the 14th and 28th days, respectively The expressions of IL-10, IL-17, IL-12, TNF-α and IFN-γ in spleen were detected by ELISA. The expressions of ROCKII, P-MYPT1, TLR4, NF-κB/p65, MCP-1, CCR2 in spleen, brain and spinal cord were detected by Western Blot. The types of macrophages and the contents of intracellular IL-10 and IL-12 were detected by Flow Cytometry. The contents of TNF-α and TLR4 mRNA in the spleen were detected by RT-PCR. Results: WYP treatment improved the clinical score of EAE mice in a significant dose-dependent manner, with the WYP high-dose group showed the most significant improvement in clinical score. Compared with the EAE control group, WYP high dose group had significantly lower levels of IL-17, IFN-γ, ROCKII, P-MYPT1, TLR4, NF-κB/p65, MCP-1, and CCR2 as well as TNF-α and TLR4 mRNA, but increased the number of M2 macrophages and IL-10. Conclusion: WYP treatment relieves clinical symptoms in EAE mice, which may be related to regulate inflammatory pathway and inhibiting expressions of inflammatory cytokines. |
| first_indexed | 2024-03-11T15:03:16Z |
| format | Article |
| id | doaj.art-eee5080cbd5146b99308035ff788384c |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T15:03:16Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-eee5080cbd5146b99308035ff788384c2023-10-30T06:06:48ZengElsevierHeliyon2405-84402023-10-01910e20621Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAEYan-Rong Li0Ruo-Nan Zhang1Rui-Rui Sun2Yan-Yan Li3Bo Zhang4Xiao-Ming Jin5Hai-Fei Zhang6Bao-Guo Xiao7Cun-Gen Ma8Hui-Jie Fan9Zhi Chai10The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, ChinaThe Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, ChinaThe Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, ChinaAffiliated Hospital of Shanxi University of Chinese Medicine, Taiyuan, 030024, ChinaHealth Commission of Shanxi Province, Taiyuan, 030001, ChinaLogical Surgery, Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN 46202, USAInstitute of Brain Science Dept, Neurology of First Affiliated Hospital, Shanxi Datong University, Datong, 037009, ChinaInstitute of Neurology, Huashan Hospital, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200025, ChinaThe Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, China; Institute of Brain Science Dept, Neurology of First Affiliated Hospital, Shanxi Datong University, Datong, 037009, China; Corresponding author. The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, China.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, China; Corresponding author.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, 030619, China; Corresponding author.Objective: Studies have shown that Wuzi Yanzong Pill (WYP) can be used to treat neurological diseases, but its mechanisms for multiple sclerosis (MS) remain unclear. This study aims to determine the effect of WYP on MS in an animal model of experimental autoimmune encephalomyelitis (EAE), and explore its mechanism. To provide theoretical basis for the clinical treatment of MS with WYP. Methods: C57BL/6 female mice were randomly divided into Blank control, EAE control, low dose WYP, medium dose WYP, and high dose WYP groups. One week before model generation, the mice were gavaged with saline (50 mL/kg/d) in Blank control and EAE control groups. The treatment groups was gavaged with different doses of WYP solution (4, 8, or 16 g/kg/d respectively) Clinical scores were recorded daily. Sample collection was conducted on the 14th and 28th days, respectively The expressions of IL-10, IL-17, IL-12, TNF-α and IFN-γ in spleen were detected by ELISA. The expressions of ROCKII, P-MYPT1, TLR4, NF-κB/p65, MCP-1, CCR2 in spleen, brain and spinal cord were detected by Western Blot. The types of macrophages and the contents of intracellular IL-10 and IL-12 were detected by Flow Cytometry. The contents of TNF-α and TLR4 mRNA in the spleen were detected by RT-PCR. Results: WYP treatment improved the clinical score of EAE mice in a significant dose-dependent manner, with the WYP high-dose group showed the most significant improvement in clinical score. Compared with the EAE control group, WYP high dose group had significantly lower levels of IL-17, IFN-γ, ROCKII, P-MYPT1, TLR4, NF-κB/p65, MCP-1, and CCR2 as well as TNF-α and TLR4 mRNA, but increased the number of M2 macrophages and IL-10. Conclusion: WYP treatment relieves clinical symptoms in EAE mice, which may be related to regulate inflammatory pathway and inhibiting expressions of inflammatory cytokines.http://www.sciencedirect.com/science/article/pii/S2405844023078295Wuzi Yanzong PillExperimental autoimmune encephalomyelitisImmunomodulationanti-inflammation |
| spellingShingle | Yan-Rong Li Ruo-Nan Zhang Rui-Rui Sun Yan-Yan Li Bo Zhang Xiao-Ming Jin Hai-Fei Zhang Bao-Guo Xiao Cun-Gen Ma Hui-Jie Fan Zhi Chai Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAE Heliyon Wuzi Yanzong Pill Experimental autoimmune encephalomyelitis Immunomodulation anti-inflammation |
| title | Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAE |
| title_full | Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAE |
| title_fullStr | Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAE |
| title_full_unstemmed | Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAE |
| title_short | Efficacy and mechanism of Wuzi Yanzong pill on the prevention and treatment of EAE |
| title_sort | efficacy and mechanism of wuzi yanzong pill on the prevention and treatment of eae |
| topic | Wuzi Yanzong Pill Experimental autoimmune encephalomyelitis Immunomodulation anti-inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023078295 |
| work_keys_str_mv | AT yanrongli efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT ruonanzhang efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT ruiruisun efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT yanyanli efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT bozhang efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT xiaomingjin efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT haifeizhang efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT baoguoxiao efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT cungenma efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT huijiefan efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae AT zhichai efficacyandmechanismofwuziyanzongpillonthepreventionandtreatmentofeae |