Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood
Background and aims: Terpenoids are one of the largest groups of natural compounds and is generally used for the treatment of human diseases e.g. taxol (anticancer drug) and artimesinin (antimalarial drug). Thus, these agents (terpenoids) derived from medicinal plant products that are effective agai...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2017-04-01
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| Series: | Advanced Herbal Medicine |
| Subjects: | |
| Online Access: | http://herbmed.skums.ac.ir/article_35212_d8ba8ec09b717655dfc60c58c1531f04.pdf |
| _version_ | 1818876092069445632 |
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| author | Amit Gupta Sushama Chaphalkar |
| author_facet | Amit Gupta Sushama Chaphalkar |
| author_sort | Amit Gupta |
| collection | DOAJ |
| description | Background and aims: Terpenoids are one of the largest groups of natural compounds and is generally used for the treatment of human diseases e.g. taxol (anticancer drug) and artimesinin (antimalarial drug). Thus, these agents (terpenoids) derived from medicinal plant products that are effective against intracellular or extracellular infections and these are urgently required. Therefore, this research was directed to assess its immunosuppressive activity of crude terpenoids on hepatitis B vaccine containing surface antigen (HBsAg) on human whole blood.Methods: In this study, crude terpenoids (6.25-25 mg/ml; 50 µl) extricate from the leaves of these medicinal plants and evaluated its immunosuppressive activity on HBsAg (20 µg/ml) using human whole blood in order to determine the analysis of blood counts (flow cytometry), cytotoxicity assay (MTT (3-[4,5-dimethylthiazol–2-yl]-2, 5-diphenyltetrazolium bromide) and nitric oxide (NO) production from cell culture supernatant.Results: The results showed that these terpenoids showed drastic decrease in monocytes and granulocytes count but increased in lymphocytes count and inhibited NO production at higher doses (25 mg/ml; 50 µl).Conclusion: These terpenoids exhibited immunosuppressive activity and could be a promising source of medicinally important natural compound. |
| first_indexed | 2024-12-19T13:36:53Z |
| format | Article |
| id | doaj.art-eee55d06b9584beb9ac9933c8ec98803 |
| institution | Directory Open Access Journal |
| issn | 2423-3579 2423-3579 |
| language | English |
| last_indexed | 2024-12-19T13:36:53Z |
| publishDate | 2017-04-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Advanced Herbal Medicine |
| spelling | doaj.art-eee55d06b9584beb9ac9933c8ec988032022-12-21T20:19:10ZengShahrekord University of Medical SciencesAdvanced Herbal Medicine2423-35792423-35792017-04-0132222835212Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole bloodAmit Gupta0Sushama Chaphalkar1VSBT, BaramatiVSBT, BaramatiBackground and aims: Terpenoids are one of the largest groups of natural compounds and is generally used for the treatment of human diseases e.g. taxol (anticancer drug) and artimesinin (antimalarial drug). Thus, these agents (terpenoids) derived from medicinal plant products that are effective against intracellular or extracellular infections and these are urgently required. Therefore, this research was directed to assess its immunosuppressive activity of crude terpenoids on hepatitis B vaccine containing surface antigen (HBsAg) on human whole blood.Methods: In this study, crude terpenoids (6.25-25 mg/ml; 50 µl) extricate from the leaves of these medicinal plants and evaluated its immunosuppressive activity on HBsAg (20 µg/ml) using human whole blood in order to determine the analysis of blood counts (flow cytometry), cytotoxicity assay (MTT (3-[4,5-dimethylthiazol–2-yl]-2, 5-diphenyltetrazolium bromide) and nitric oxide (NO) production from cell culture supernatant.Results: The results showed that these terpenoids showed drastic decrease in monocytes and granulocytes count but increased in lymphocytes count and inhibited NO production at higher doses (25 mg/ml; 50 µl).Conclusion: These terpenoids exhibited immunosuppressive activity and could be a promising source of medicinally important natural compound.http://herbmed.skums.ac.ir/article_35212_d8ba8ec09b717655dfc60c58c1531f04.pdfimmunopharmacologicalmesua ferreaficus benghalensisbutea frondosa |
| spellingShingle | Amit Gupta Sushama Chaphalkar Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood Advanced Herbal Medicine immunopharmacological mesua ferrea ficus benghalensis butea frondosa |
| title | Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood |
| title_full | Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood |
| title_fullStr | Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood |
| title_full_unstemmed | Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood |
| title_short | Immunosuppressive activity of terpenoids from Mesua ferrea, Ficus benghalensis and Butea frondosa on human whole blood |
| title_sort | immunosuppressive activity of terpenoids from mesua ferrea ficus benghalensis and butea frondosa on human whole blood |
| topic | immunopharmacological mesua ferrea ficus benghalensis butea frondosa |
| url | http://herbmed.skums.ac.ir/article_35212_d8ba8ec09b717655dfc60c58c1531f04.pdf |
| work_keys_str_mv | AT amitgupta immunosuppressiveactivityofterpenoidsfrommesuaferreaficusbenghalensisandbuteafrondosaonhumanwholeblood AT sushamachaphalkar immunosuppressiveactivityofterpenoidsfrommesuaferreaficusbenghalensisandbuteafrondosaonhumanwholeblood |