<i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in Rice
Leaf-color mutants in rice (<i>Oryza sativa</i> L.) are excellent models for studying chlorophyll biosynthesis and chloroplast development. In this study, a yellow-green-leaf mutant generated by <sup>60</sup>Co irradiation, <i>ygl9311</i>, was isolated: it display...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/1/129 |
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| author | Wei Lu Yantong Teng Fushou He Xue Wang Yonghua Qin Gang Cheng Xin Xu Chuntai Wang Yanping Tan |
| author_facet | Wei Lu Yantong Teng Fushou He Xue Wang Yonghua Qin Gang Cheng Xin Xu Chuntai Wang Yanping Tan |
| author_sort | Wei Lu |
| collection | DOAJ |
| description | Leaf-color mutants in rice (<i>Oryza sativa</i> L.) are excellent models for studying chlorophyll biosynthesis and chloroplast development. In this study, a yellow-green-leaf mutant generated by <sup>60</sup>Co irradiation, <i>ygl9311</i>, was isolated: it displayed a yellow-green leaf phenotype during the complete growth cycle. Compared with the wild type, the photosynthetic pigment contents of leaves in <i>ygl9311</i> were significantly reduced, and chloroplast development was retarded. Genetic analysis indicated that the <i>ygl9311</i> phenotype was controlled by a single recessive nuclear gene. Map-based cloning and transcriptome sequencing analysis suggested that the candidate gene was <i>OsChlC1</i> (<i>BGIOSGA012976</i>), which encodes a Mg-chelatase I subunit. The results of CRISPR/Cas9 system and RNAi knockout tests show that mutation of <i>OsChlC1</i> could reproduce the phenotype of yellow-green leaves of the mutant <i>ygl9311</i>. In conclusion, the novel rice leaf-color gene <i>OsChlC1</i> affects the content of chlorophyll in rice, showing a relatively conserved function in <i>indica</i> and <i>japonica</i> rice cultivars. |
| first_indexed | 2024-03-09T13:51:37Z |
| format | Article |
| id | doaj.art-eee98c1fa4014fe693931c84aae528d2 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:51:37Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-eee98c1fa4014fe693931c84aae528d22023-11-30T20:49:20ZengMDPI AGAgronomy2073-43952022-12-0113112910.3390/agronomy13010129<i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in RiceWei Lu0Yantong Teng1Fushou He2Xue Wang3Yonghua Qin4Gang Cheng5Xin Xu6Chuntai Wang7Yanping Tan8Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central Minzu University, Wuhan 430074, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Science, South-Central Minzu University, Wuhan 430074, ChinaCollege of Life Science, South-Central Minzu University, Wuhan 430074, ChinaHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central Minzu University, Wuhan 430074, ChinaHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central Minzu University, Wuhan 430074, ChinaHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central Minzu University, Wuhan 430074, ChinaHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central Minzu University, Wuhan 430074, ChinaHubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central Minzu University, Wuhan 430074, ChinaLeaf-color mutants in rice (<i>Oryza sativa</i> L.) are excellent models for studying chlorophyll biosynthesis and chloroplast development. In this study, a yellow-green-leaf mutant generated by <sup>60</sup>Co irradiation, <i>ygl9311</i>, was isolated: it displayed a yellow-green leaf phenotype during the complete growth cycle. Compared with the wild type, the photosynthetic pigment contents of leaves in <i>ygl9311</i> were significantly reduced, and chloroplast development was retarded. Genetic analysis indicated that the <i>ygl9311</i> phenotype was controlled by a single recessive nuclear gene. Map-based cloning and transcriptome sequencing analysis suggested that the candidate gene was <i>OsChlC1</i> (<i>BGIOSGA012976</i>), which encodes a Mg-chelatase I subunit. The results of CRISPR/Cas9 system and RNAi knockout tests show that mutation of <i>OsChlC1</i> could reproduce the phenotype of yellow-green leaves of the mutant <i>ygl9311</i>. In conclusion, the novel rice leaf-color gene <i>OsChlC1</i> affects the content of chlorophyll in rice, showing a relatively conserved function in <i>indica</i> and <i>japonica</i> rice cultivars.https://www.mdpi.com/2073-4395/13/1/129riceleaf-color mutantschlorophyll<i>ygl9311</i><i>OsChlC1</i>Mg-chelatase |
| spellingShingle | Wei Lu Yantong Teng Fushou He Xue Wang Yonghua Qin Gang Cheng Xin Xu Chuntai Wang Yanping Tan <i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in Rice Agronomy rice leaf-color mutants chlorophyll <i>ygl9311</i> <i>OsChlC1</i> Mg-chelatase |
| title | <i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in Rice |
| title_full | <i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in Rice |
| title_fullStr | <i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in Rice |
| title_full_unstemmed | <i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in Rice |
| title_short | <i>OsChlC1</i>, a Novel Gene Encoding Magnesium-Chelating Enzyme, Affects the Content of Chlorophyll in Rice |
| title_sort | i oschlc1 i a novel gene encoding magnesium chelating enzyme affects the content of chlorophyll in rice |
| topic | rice leaf-color mutants chlorophyll <i>ygl9311</i> <i>OsChlC1</i> Mg-chelatase |
| url | https://www.mdpi.com/2073-4395/13/1/129 |
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