Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease
Abstract Background Kawasaki disease is an autoimmune disease characterized by systemic vasculitis of unknown aetiology and most commonly occurs in children under 5 years old. Previous studies have found that the over-activation of lymphocytes is an important mechanism of Kawasaki disease. Activin A...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | BMC Immunology |
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| Online Access: | https://doi.org/10.1186/s12865-021-00407-x |
| _version_ | 1818328883326353408 |
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| author | Qian Wu Ruohang Weng Yongbin Xu Linlin Wang Yanyan Huang Jun Yang |
| author_facet | Qian Wu Ruohang Weng Yongbin Xu Linlin Wang Yanyan Huang Jun Yang |
| author_sort | Qian Wu |
| collection | DOAJ |
| description | Abstract Background Kawasaki disease is an autoimmune disease characterized by systemic vasculitis of unknown aetiology and most commonly occurs in children under 5 years old. Previous studies have found that the over-activation of lymphocytes is an important mechanism of Kawasaki disease. Activin A, also known as immunosuppressive factor P, is a multifunctional growth and transforming factor. However, whether activin A is involved in the regulation of peripheral lymphocytes activity in Kawasaki disease is unclear. Thus, we aimed to investigate the effect of activin A on the activity of peripheral lymphocytes in acute-phase Kawasaki disease. Methods Seven patients with Kawasaki disease and seven healthy controls were studied. Peripheral blood lymphocytes were isolated by Ficoll density gradient centrifugation. The activation of CD4+ and CD8+ T cells and CD19+ B cells was investigated by flow cytometry. The expression of activin type IIA receptors was investigated by flow cytometry. Results Immune imbalance in CD4 and CD8 lymphocytes were detected in acute-phase Kawasaki disease. The expression of activin type IIA receptors on CD8+ T cells and CD19+ B cells was increased in acute-phase Kawasaki disease and decreased following treatment with activin A. Activin A suppressed the expression of CD25 and CD69 on CD8+ T cells and the expression of CD69 on CD19+ B cells. Conclusions The expression of activin type IIA receptor was increased on CD8+ T cells and CD19+ B cells in Kawasaki disease. Activin A suppressed the expression of CD25, CD69 and activin type IIA receptors on peripheral CD8+ T lymphocyte. Activin A plays different roles in different lymphocyte subsets and suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease. |
| first_indexed | 2024-12-13T12:39:14Z |
| format | Article |
| id | doaj.art-eee9dced049544808d5e64906e0b3dc3 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-13T12:39:14Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-eee9dced049544808d5e64906e0b3dc32022-12-21T23:45:42ZengBMCBMC Immunology1471-21722021-02-012211710.1186/s12865-021-00407-xActivin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki diseaseQian Wu0Ruohang Weng1Yongbin Xu2Linlin Wang3Yanyan Huang4Jun Yang5State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Genome, Peking University, Shenzhen Graduate School, School of Chemical Biology & BiotechnologyDepartment of Rheumatology and Immunology, Shenzhen Children’s HospitalDepartment of Rheumatology and Immunology, Shenzhen Children’s HospitalDepartment of Rheumatology and Immunology, Shenzhen Children’s HospitalDepartment of Rheumatology and Immunology, Shenzhen Children’s HospitalDepartment of Rheumatology and Immunology, Shenzhen Children’s HospitalAbstract Background Kawasaki disease is an autoimmune disease characterized by systemic vasculitis of unknown aetiology and most commonly occurs in children under 5 years old. Previous studies have found that the over-activation of lymphocytes is an important mechanism of Kawasaki disease. Activin A, also known as immunosuppressive factor P, is a multifunctional growth and transforming factor. However, whether activin A is involved in the regulation of peripheral lymphocytes activity in Kawasaki disease is unclear. Thus, we aimed to investigate the effect of activin A on the activity of peripheral lymphocytes in acute-phase Kawasaki disease. Methods Seven patients with Kawasaki disease and seven healthy controls were studied. Peripheral blood lymphocytes were isolated by Ficoll density gradient centrifugation. The activation of CD4+ and CD8+ T cells and CD19+ B cells was investigated by flow cytometry. The expression of activin type IIA receptors was investigated by flow cytometry. Results Immune imbalance in CD4 and CD8 lymphocytes were detected in acute-phase Kawasaki disease. The expression of activin type IIA receptors on CD8+ T cells and CD19+ B cells was increased in acute-phase Kawasaki disease and decreased following treatment with activin A. Activin A suppressed the expression of CD25 and CD69 on CD8+ T cells and the expression of CD69 on CD19+ B cells. Conclusions The expression of activin type IIA receptor was increased on CD8+ T cells and CD19+ B cells in Kawasaki disease. Activin A suppressed the expression of CD25, CD69 and activin type IIA receptors on peripheral CD8+ T lymphocyte. Activin A plays different roles in different lymphocyte subsets and suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease.https://doi.org/10.1186/s12865-021-00407-xKawasaki diseaseActivin type IIA receptorPeripheral lymphocytesActivin a |
| spellingShingle | Qian Wu Ruohang Weng Yongbin Xu Linlin Wang Yanyan Huang Jun Yang Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease BMC Immunology Kawasaki disease Activin type IIA receptor Peripheral lymphocytes Activin a |
| title | Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease |
| title_full | Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease |
| title_fullStr | Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease |
| title_full_unstemmed | Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease |
| title_short | Activin a suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease |
| title_sort | activin a suppresses peripheral cd8 t lymphocyte activity in acute phase kawasaki disease |
| topic | Kawasaki disease Activin type IIA receptor Peripheral lymphocytes Activin a |
| url | https://doi.org/10.1186/s12865-021-00407-x |
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