Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian population
Background and Objective: Cyclins are the key regulator of the cell cycle and their over-expression has been seen in many cancers including breast cancer. Cyclin D1 is an oncoprotein encoded by CCND1 gene located on chromosome 11 (11q) which regulates cell cycle in shifting from G1 to S phase. It’s...
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| Format: | Article |
| Language: | English |
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"Alexandru Ioan Cuza" University of Iași
2024-03-01
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| Series: | Journal of Experimental and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/153 |
| _version_ | 1797260173043040256 |
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| author | Khosrow Keshavarz Safiei Farhad Mashayekhi Hamid Saeidi saedi |
| author_facet | Khosrow Keshavarz Safiei Farhad Mashayekhi Hamid Saeidi saedi |
| author_sort | Khosrow Keshavarz Safiei |
| collection | DOAJ |
| description |
Background and Objective: Cyclins are the key regulator of the cell cycle and their over-expression has been seen in many cancers including breast cancer. Cyclin D1 is an oncoprotein encoded by CCND1 gene located on chromosome 11 (11q) which regulates cell cycle in shifting from G1 to S phase. It’s the main target for steroids and mitogenic growth hormones in breast epithelial cells. This study aimed to evaluate the relationship between Cyclin D1 G870A polymorphism and breast cancer risk in a population in the north of Iran.
Methods: Whole blood samples collected from 82 patients with breast cancer and 66 healthy women. DNA was extracted and genotyping was performed by Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) technique.
Results: Genotypic prevalence of AA, AG, GG genotypes among patients were 40.2%, 35.3% and 24.4% and in controls were 30%, 47%, 23%, respectively. There was no significant difference in CCND1 G870A genotype polymorphism between patients and control group (p=0.32). Also, allelic prevalence of A and G alleles in breast cancer patients were 58% and 42%, in controls were 54% and 46%, respectively. The present study showed that there is no significant association between CCND1 G870A polymorphism with the risk of breast cancer.
Conclusion: The results of this study revealed that there is no significant association between CCND1 G870A genetic polymorphism and the risk of breast cancer in the population of the north of Iran. More studies with larger samples of cases and controls would be beneficial.
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| first_indexed | 2024-04-24T23:21:06Z |
| format | Article |
| id | doaj.art-eeec617c0c004c4eac80302701b31027 |
| institution | Directory Open Access Journal |
| issn | 2601-6974 |
| language | English |
| last_indexed | 2024-04-24T23:21:06Z |
| publishDate | 2024-03-01 |
| publisher | "Alexandru Ioan Cuza" University of Iași |
| record_format | Article |
| series | Journal of Experimental and Molecular Biology |
| spelling | doaj.art-eeec617c0c004c4eac80302701b310272024-03-16T11:02:24Zeng"Alexandru Ioan Cuza" University of IașiJournal of Experimental and Molecular Biology2601-69742024-03-0110.47743/jemb-2024-153Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian populationKhosrow Keshavarz Safiei0Farhad MashayekhiHamid Saeidi saedi Department of Genetics Background and Objective: Cyclins are the key regulator of the cell cycle and their over-expression has been seen in many cancers including breast cancer. Cyclin D1 is an oncoprotein encoded by CCND1 gene located on chromosome 11 (11q) which regulates cell cycle in shifting from G1 to S phase. It’s the main target for steroids and mitogenic growth hormones in breast epithelial cells. This study aimed to evaluate the relationship between Cyclin D1 G870A polymorphism and breast cancer risk in a population in the north of Iran. Methods: Whole blood samples collected from 82 patients with breast cancer and 66 healthy women. DNA was extracted and genotyping was performed by Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) technique. Results: Genotypic prevalence of AA, AG, GG genotypes among patients were 40.2%, 35.3% and 24.4% and in controls were 30%, 47%, 23%, respectively. There was no significant difference in CCND1 G870A genotype polymorphism between patients and control group (p=0.32). Also, allelic prevalence of A and G alleles in breast cancer patients were 58% and 42%, in controls were 54% and 46%, respectively. The present study showed that there is no significant association between CCND1 G870A polymorphism with the risk of breast cancer. Conclusion: The results of this study revealed that there is no significant association between CCND1 G870A genetic polymorphism and the risk of breast cancer in the population of the north of Iran. More studies with larger samples of cases and controls would be beneficial. http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/153Breast cancer; ccnd1; polymorphism |
| spellingShingle | Khosrow Keshavarz Safiei Farhad Mashayekhi Hamid Saeidi saedi Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian population Journal of Experimental and Molecular Biology Breast cancer; ccnd1; polymorphism |
| title | Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian population |
| title_full | Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian population |
| title_fullStr | Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian population |
| title_full_unstemmed | Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian population |
| title_short | Cyclin D1 (G870A) polymorphism and breast cancer risk in an Iranian population |
| title_sort | cyclin d1 g870a polymorphism and breast cancer risk in an iranian population |
| topic | Breast cancer; ccnd1; polymorphism |
| url | http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/153 |
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