An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT Italiko
Sweet basil (<i>Ocimum basilicum</i> L.; Fam. Lamiaceae) is an annual herbaceous plant with a high economic value used in folk medicine, pharmacology, and food production. In Italy, most of the varieties are used to produce the famous “pesto” sauce; however, almost all of them are suscep...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/9/3/407 |
| _version_ | 1797611416753012736 |
|---|---|
| author | Sara Barberini Chiara Forti Marina Laura Roberto Ciorba Carlo Mascarello Annalisa Giovannini Barbara Ruffoni Marco Savona |
| author_facet | Sara Barberini Chiara Forti Marina Laura Roberto Ciorba Carlo Mascarello Annalisa Giovannini Barbara Ruffoni Marco Savona |
| author_sort | Sara Barberini |
| collection | DOAJ |
| description | Sweet basil (<i>Ocimum basilicum</i> L.; Fam. Lamiaceae) is an annual herbaceous plant with a high economic value used in folk medicine, pharmacology, and food production. In Italy, most of the varieties are used to produce the famous “pesto” sauce; however, almost all of them are susceptible to basil downy mildew (BDM) disease, strongly decreasing the growth of the fresh leaves and the survival of the whole plant. Nowadays, CRISPR/Cas9 technology is recognized to be a prominent way to enhance basil genetic breeding. In this work, we present an optimized protocol for in vitro direct regeneration of an elite cultivar, which is the major limiting factor for the transformation of <i>O. basilicum</i>. Regeneration has been obtained from different explants (leaves, cotyledons, cotyledonary nodes); the highest frequency has been obtained from cotyledonary nodes of seedlings germinated on MS medium containing TDZ. This protocol may be used for biotechnological applications as genome editing techniques to obtain basil-downy-mildew-disease-resistant clones. |
| first_indexed | 2024-03-11T06:28:36Z |
| format | Article |
| id | doaj.art-eeee2e04fac04396ac149386a7f1c2f9 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T06:28:36Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-eeee2e04fac04396ac149386a7f1c2f92023-11-17T11:25:13ZengMDPI AGHorticulturae2311-75242023-03-019340710.3390/horticulturae9030407An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT ItalikoSara Barberini0Chiara Forti1Marina Laura2Roberto Ciorba3Carlo Mascarello4Annalisa Giovannini5Barbara Ruffoni6Marco Savona7Institute for Sustainable Plant Protection (IPSP CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, ItalyInstitute of Agricultural Biology and Biotechnology (IBBA CNR), Via Bassini 12, 20133 Milano, ItalyCREA Research Centre for Vegetable and Ornamental Crops (CREA OF), Corso degli Inglesi 508, 18038 Sanremo, ItalyCREA Research Centre for Olive, Fruit and Citrus Crops, Via di Fioranello 52, 00134 Rome, ItalyCREA Research Centre for Vegetable and Ornamental Crops (CREA OF), Corso degli Inglesi 508, 18038 Sanremo, ItalyCREA Research Centre for Vegetable and Ornamental Crops (CREA OF), Corso degli Inglesi 508, 18038 Sanremo, ItalyCREA Research Centre for Vegetable and Ornamental Crops (CREA OF), Corso degli Inglesi 508, 18038 Sanremo, ItalyCREA Research Centre for Vegetable and Ornamental Crops (CREA OF), Corso degli Inglesi 508, 18038 Sanremo, ItalySweet basil (<i>Ocimum basilicum</i> L.; Fam. Lamiaceae) is an annual herbaceous plant with a high economic value used in folk medicine, pharmacology, and food production. In Italy, most of the varieties are used to produce the famous “pesto” sauce; however, almost all of them are susceptible to basil downy mildew (BDM) disease, strongly decreasing the growth of the fresh leaves and the survival of the whole plant. Nowadays, CRISPR/Cas9 technology is recognized to be a prominent way to enhance basil genetic breeding. In this work, we present an optimized protocol for in vitro direct regeneration of an elite cultivar, which is the major limiting factor for the transformation of <i>O. basilicum</i>. Regeneration has been obtained from different explants (leaves, cotyledons, cotyledonary nodes); the highest frequency has been obtained from cotyledonary nodes of seedlings germinated on MS medium containing TDZ. This protocol may be used for biotechnological applications as genome editing techniques to obtain basil-downy-mildew-disease-resistant clones.https://www.mdpi.com/2311-7524/9/3/407sweet basil“pesto” saucecotyledonary nodesdirect regeneration |
| spellingShingle | Sara Barberini Chiara Forti Marina Laura Roberto Ciorba Carlo Mascarello Annalisa Giovannini Barbara Ruffoni Marco Savona An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT Italiko Horticulturae sweet basil “pesto” sauce cotyledonary nodes direct regeneration |
| title | An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT Italiko |
| title_full | An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT Italiko |
| title_fullStr | An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT Italiko |
| title_full_unstemmed | An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT Italiko |
| title_short | An Optimized Protocol for In Vitro Regeneration of <i>Ocimum basilicum</i> cv. FT Italiko |
| title_sort | optimized protocol for in vitro regeneration of i ocimum basilicum i cv ft italiko |
| topic | sweet basil “pesto” sauce cotyledonary nodes direct regeneration |
| url | https://www.mdpi.com/2311-7524/9/3/407 |
| work_keys_str_mv | AT sarabarberini anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT chiaraforti anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT marinalaura anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT robertociorba anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT carlomascarello anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT annalisagiovannini anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT barbararuffoni anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT marcosavona anoptimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT sarabarberini optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT chiaraforti optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT marinalaura optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT robertociorba optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT carlomascarello optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT annalisagiovannini optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT barbararuffoni optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko AT marcosavona optimizedprotocolforinvitroregenerationofiocimumbasilicumicvftitaliko |