Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme
The present study aims to identify the diverse endophytic fungi residing in the roots of Dendrobium moniliforme and their role in plant growth and development. Nine endophytic fungi were isolated from the root sections and characterized by molecular technique. Quantification of the indole acetic aci...
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| Format: | Article |
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MDPI AG
2018-12-01
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| Series: | Plants |
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| Online Access: | http://www.mdpi.com/2223-7747/8/1/5 |
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| author | Sujit Shah Roshani Shrestha Sabitri Maharjan Marc-Andre Selosse Bijaya Pant |
| author_facet | Sujit Shah Roshani Shrestha Sabitri Maharjan Marc-Andre Selosse Bijaya Pant |
| author_sort | Sujit Shah |
| collection | DOAJ |
| description | The present study aims to identify the diverse endophytic fungi residing in the roots of Dendrobium moniliforme and their role in plant growth and development. Nine endophytic fungi were isolated from the root sections and characterized by molecular technique. Quantification of the indole acetic acid (IAA) compound by these endophytes was done. Further, Chemical profiling of R11 and R13 fungi was done by Gas Chromatography-Mass Spectroscopy (GC-MS). Asymbiotic seed derived protocorms of Rhynchostylis retusa was used for the plant growth assay to investigate the growth promoting activities of the fungal elicitor prepared from the isolated fungi from D. moniliforme. Among the isolated fungi, the relative dominant fungus was Fusarium sp. The R13 and R6 fungi were identified only at the genus level which concludes the fungi are of new species or strain. The indole acetic acid production was relatively higher in R10. Bioactive compound diversity was observed in the organic extract of R11 and R6. The presence of phenolic compound and essential oil suggest their contribution for the antimicrobial and antioxidant properties to their host plant, D. moniliforme. The plant growth assay result concluded, the fungal elicitor prepared from R10, Colletotrichum alatae was the best among all other for the plant growth activities. |
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| id | doaj.art-eeef77893cdd4905906990eeaed9b266 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-24T04:23:30Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-eeef77893cdd4905906990eeaed9b2662022-12-21T17:15:43ZengMDPI AGPlants2223-77472018-12-0181510.3390/plants8010005plants8010005Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliformeSujit Shah0Roshani Shrestha1Sabitri Maharjan2Marc-Andre Selosse3Bijaya Pant4Central Department of Botany, Tribhuvan University, Kathmandu 2642, NepalCentral Department of Botany, Tribhuvan University, Kathmandu 2642, NepalCentral Department of Botany, Tribhuvan University, Kathmandu 2642, NepalInstitut de Systématique, Évolution, Biodiversité (UMR 7205—CNRS, MNHN, UPMC, EPHE), Muséum National d’Histoire naturelle, Sorbonne Universités, 57 rue Cuvier, 75005 Paris, FranceCentral Department of Botany, Tribhuvan University, Kathmandu 2642, NepalThe present study aims to identify the diverse endophytic fungi residing in the roots of Dendrobium moniliforme and their role in plant growth and development. Nine endophytic fungi were isolated from the root sections and characterized by molecular technique. Quantification of the indole acetic acid (IAA) compound by these endophytes was done. Further, Chemical profiling of R11 and R13 fungi was done by Gas Chromatography-Mass Spectroscopy (GC-MS). Asymbiotic seed derived protocorms of Rhynchostylis retusa was used for the plant growth assay to investigate the growth promoting activities of the fungal elicitor prepared from the isolated fungi from D. moniliforme. Among the isolated fungi, the relative dominant fungus was Fusarium sp. The R13 and R6 fungi were identified only at the genus level which concludes the fungi are of new species or strain. The indole acetic acid production was relatively higher in R10. Bioactive compound diversity was observed in the organic extract of R11 and R6. The presence of phenolic compound and essential oil suggest their contribution for the antimicrobial and antioxidant properties to their host plant, D. moniliforme. The plant growth assay result concluded, the fungal elicitor prepared from R10, Colletotrichum alatae was the best among all other for the plant growth activities.http://www.mdpi.com/2223-7747/8/1/5orchidDendrobium moniliformeendophyteselicitorindole acetic acid |
| spellingShingle | Sujit Shah Roshani Shrestha Sabitri Maharjan Marc-Andre Selosse Bijaya Pant Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme Plants orchid Dendrobium moniliforme endophytes elicitor indole acetic acid |
| title | Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme |
| title_full | Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme |
| title_fullStr | Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme |
| title_full_unstemmed | Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme |
| title_short | Isolation and Characterization of Plant Growth-Promoting Endophytic Fungi from the Roots of Dendrobium moniliforme |
| title_sort | isolation and characterization of plant growth promoting endophytic fungi from the roots of dendrobium moniliforme |
| topic | orchid Dendrobium moniliforme endophytes elicitor indole acetic acid |
| url | http://www.mdpi.com/2223-7747/8/1/5 |
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