Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices
ABSTRACT: Mastitis in cattle is a major health problem as well as incurring high costs for the dairy industry. To assess the suitability of precision-cut bovine udder slices (PCBUS) for bovine mastitis studies, we infected PCBUS with 2 different Staphylococcus aureus strains. Accordingly, we investi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030222004106 |
| _version_ | 1798037419995430912 |
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| author | V. Filor B. Seeger N. de Buhr M. von Köckritz-Blickwede M. Kietzmann H. Oltmanns J. Meißner |
| author_facet | V. Filor B. Seeger N. de Buhr M. von Köckritz-Blickwede M. Kietzmann H. Oltmanns J. Meißner |
| author_sort | V. Filor |
| collection | DOAJ |
| description | ABSTRACT: Mastitis in cattle is a major health problem as well as incurring high costs for the dairy industry. To assess the suitability of precision-cut bovine udder slices (PCBUS) for bovine mastitis studies, we infected PCBUS with 2 different Staphylococcus aureus strains. Accordingly, we investigated both the tissue response to infection based on immune mediators at the mRNA and protein levels and the invasion of bacteria within the tissue. The studied proteins represent immune mediators of early inflammation [IL-1β, tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2)] and showed a time-dependent increase in concentration. Infection of PCBUS with S. aureus resulted in increased expression of proinflammatory cytokines and chemokines such as TNF-α, C-C motif chemokine ligand 20 (CCL20), IL-1β, IL-6, and IL-10, but not C-X-C motif chemokine ligand 8 (CXCL8), lingual antimicrobial peptide (LAP), or S100 calcium binding protein A9 (S100A9) at the mRNA level. To compare the data acquired with this model, we carried out investigations on primary bovine mammary epithelial cells. Our results showed that the immune responses of both models—PCBUS and primary bovine mammary epithelial cells—were similar. In addition, investigations using PCBUS enabled us to demonstrate adherence of bacteria in the physiological cell network. These findings support the use of PCBUS in studies designed to further understand the complex pathophysiological processes of infection and inflammation in bovine mastitis and to investigate alternative therapies for mastitis. |
| first_indexed | 2024-04-11T21:26:17Z |
| format | Article |
| id | doaj.art-eef2fac854754e4e8386bc918c9dca18 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-04-11T21:26:17Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-eef2fac854754e4e8386bc918c9dca182022-12-22T04:02:24ZengElsevierJournal of Dairy Science0022-03022022-09-01105977057718Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slicesV. Filor0B. Seeger1N. de Buhr2M. von Köckritz-Blickwede3M. Kietzmann4H. Oltmanns5J. Meißner6Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany; Department of Veterinary Medicine, Institute of Pharmacology and Toxicology, Freie Universität Berlin, Koserstraße 20, 14195 Berlin, Germany; Corresponding authorInstitute for Food Quality and Food Safety, Research Group Food Toxicology/Alternative/Complementary Methods to Animal Testing, University of Veterinary Medicine, 30273 Hannover, GermanyDepartment of Biochemistry, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany; Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, GermanyDepartment of Biochemistry, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany; Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, GermanyDepartment of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, GermanyDepartment of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, GermanyDepartment of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, GermanyABSTRACT: Mastitis in cattle is a major health problem as well as incurring high costs for the dairy industry. To assess the suitability of precision-cut bovine udder slices (PCBUS) for bovine mastitis studies, we infected PCBUS with 2 different Staphylococcus aureus strains. Accordingly, we investigated both the tissue response to infection based on immune mediators at the mRNA and protein levels and the invasion of bacteria within the tissue. The studied proteins represent immune mediators of early inflammation [IL-1β, tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2)] and showed a time-dependent increase in concentration. Infection of PCBUS with S. aureus resulted in increased expression of proinflammatory cytokines and chemokines such as TNF-α, C-C motif chemokine ligand 20 (CCL20), IL-1β, IL-6, and IL-10, but not C-X-C motif chemokine ligand 8 (CXCL8), lingual antimicrobial peptide (LAP), or S100 calcium binding protein A9 (S100A9) at the mRNA level. To compare the data acquired with this model, we carried out investigations on primary bovine mammary epithelial cells. Our results showed that the immune responses of both models—PCBUS and primary bovine mammary epithelial cells—were similar. In addition, investigations using PCBUS enabled us to demonstrate adherence of bacteria in the physiological cell network. These findings support the use of PCBUS in studies designed to further understand the complex pathophysiological processes of infection and inflammation in bovine mastitis and to investigate alternative therapies for mastitis.http://www.sciencedirect.com/science/article/pii/S0022030222004106bovine mastitisprecision-cut bovine udder slicesprimary bovine mammary epithelial cellsStaphylococcus aureusimmune response |
| spellingShingle | V. Filor B. Seeger N. de Buhr M. von Köckritz-Blickwede M. Kietzmann H. Oltmanns J. Meißner Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices Journal of Dairy Science bovine mastitis precision-cut bovine udder slices primary bovine mammary epithelial cells Staphylococcus aureus immune response |
| title | Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices |
| title_full | Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices |
| title_fullStr | Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices |
| title_full_unstemmed | Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices |
| title_short | Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices |
| title_sort | investigation of the pathophysiology of bacterial mastitis using precision cut bovine udder slices |
| topic | bovine mastitis precision-cut bovine udder slices primary bovine mammary epithelial cells Staphylococcus aureus immune response |
| url | http://www.sciencedirect.com/science/article/pii/S0022030222004106 |
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