Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq
Genetic variety examination has demonstrated fundamental to the understanding of the epidemiological and developmental history of Papillomavirus (HPV), for the development of accurate diagnostic tests and for efficient vaccine design. The HPV nucleotide diversity has been investigated widely among h...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
University of Thi-Qar
2020-12-01
|
Series: | مجلة علوم ذي قار |
Subjects: | |
Online Access: | https://jsci.utq.edu.iq/index.php/main/article/view/709 |
_version_ | 1797632444991537152 |
---|---|
author | Bushra Jabbar Hamad Manal Badi Saleh |
author_facet | Bushra Jabbar Hamad Manal Badi Saleh |
author_sort | Bushra Jabbar Hamad |
collection | DOAJ |
description | Genetic variety examination has demonstrated fundamental to the understanding of the epidemiological and developmental history of Papillomavirus (HPV), for the development of accurate diagnostic tests and for efficient vaccine design. The HPV nucleotide diversity has been investigated widely among high-risk HPV types. To make the nucleotide sequence of HPV and do the virus database in Thi-Qar province, and compare sequences of our isolates with previously described isolates from around the world and then draw its phylogenetic tree, this study done. A total of 6 breast formalin-fixed paraffin-embedded (FFPE) of the female patients were included in the study, divided as 4 FFPE malignant tumor and 2 FFPE of benign tumor. The PCR technique was implemented to detect the presence of HPV in breast tissue, and the real-time PCR used to determinant HPV genotypes, then determined a complete nucleotide sequence of HPV of L1 capsid gene, and draw its phylogenetic tree. The nucleotide sequencing finding detects a number of substitution mutation (SNPs) in (L1) gene, which have not been designated before, were identified once in this study population, and revealed that the HPV16 strains have the evolutionary relationship with the South African race, while, the HPV33 and HPV6 showing the evolutionary association with the North American and East Asian race, respectively.
|
first_indexed | 2024-03-11T11:37:09Z |
format | Article |
id | doaj.art-eef45d348d3a4026a3ed79aba357928c |
institution | Directory Open Access Journal |
issn | 1991-8690 2709-0256 |
language | English |
last_indexed | 2024-03-11T11:37:09Z |
publishDate | 2020-12-01 |
publisher | University of Thi-Qar |
record_format | Article |
series | مجلة علوم ذي قار |
spelling | doaj.art-eef45d348d3a4026a3ed79aba357928c2023-11-10T13:21:15ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562020-12-0172Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/IraqBushra Jabbar HamadManal Badi SalehGenetic variety examination has demonstrated fundamental to the understanding of the epidemiological and developmental history of Papillomavirus (HPV), for the development of accurate diagnostic tests and for efficient vaccine design. The HPV nucleotide diversity has been investigated widely among high-risk HPV types. To make the nucleotide sequence of HPV and do the virus database in Thi-Qar province, and compare sequences of our isolates with previously described isolates from around the world and then draw its phylogenetic tree, this study done. A total of 6 breast formalin-fixed paraffin-embedded (FFPE) of the female patients were included in the study, divided as 4 FFPE malignant tumor and 2 FFPE of benign tumor. The PCR technique was implemented to detect the presence of HPV in breast tissue, and the real-time PCR used to determinant HPV genotypes, then determined a complete nucleotide sequence of HPV of L1 capsid gene, and draw its phylogenetic tree. The nucleotide sequencing finding detects a number of substitution mutation (SNPs) in (L1) gene, which have not been designated before, were identified once in this study population, and revealed that the HPV16 strains have the evolutionary relationship with the South African race, while, the HPV33 and HPV6 showing the evolutionary association with the North American and East Asian race, respectively. https://jsci.utq.edu.iq/index.php/main/article/view/709HPV, Sequencing, Phylogenetic tree, Sanger, Breast Cancer words) |
spellingShingle | Bushra Jabbar Hamad Manal Badi Saleh Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq مجلة علوم ذي قار HPV, Sequencing, Phylogenetic tree, Sanger, Breast Cancer words) |
title | Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq |
title_full | Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq |
title_fullStr | Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq |
title_full_unstemmed | Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq |
title_short | Sequencing Analysis and Phylogenetic Tree of HPV Isolated from Breast Cancer Patients at Thi-Qar Province/Iraq |
title_sort | sequencing analysis and phylogenetic tree of hpv isolated from breast cancer patients at thi qar province iraq |
topic | HPV, Sequencing, Phylogenetic tree, Sanger, Breast Cancer words) |
url | https://jsci.utq.edu.iq/index.php/main/article/view/709 |
work_keys_str_mv | AT bushrajabbarhamad sequencinganalysisandphylogenetictreeofhpvisolatedfrombreastcancerpatientsatthiqarprovinceiraq AT manalbadisaleh sequencinganalysisandphylogenetictreeofhpvisolatedfrombreastcancerpatientsatthiqarprovinceiraq |