cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi
Pattern recognition receptors play an important role in insect immune defense. We cloned the β-1,3-glucan recognition protein, lectin-5 and C-type lectin 1 genes of Antheraea pernyi and examined the expression profiles of immune-stimulated pupae. After infection with Bacillus subtilis,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-10-01
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| Series: | Insects |
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| Online Access: | http://www.mdpi.com/2075-4450/3/4/1093 |
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| author | Wenli Li Suyun Fang Yuan Li Fengjuan Li Olle Terenius |
| author_facet | Wenli Li Suyun Fang Yuan Li Fengjuan Li Olle Terenius |
| author_sort | Wenli Li |
| collection | DOAJ |
| description | Pattern recognition receptors play an important role in insect immune defense. We cloned the β-1,3-glucan recognition protein, lectin-5 and C-type lectin 1 genes of Antheraea pernyi and examined the expression profiles of immune-stimulated pupae. After infection with Bacillus subtilis, Escherichia coli, Antheraea pernyi nuclear polyhedrosis virus (ApNPV) and Saccharomyces cerevisiae, respectively, the pupae showed different gene expression levels in the different tissues examined (midgut, fatbody, epidermis, testis, and hemocytes). ApβGRP and Aplectin-5 was induced by all the microorganisms, and mainly in epidermis and hemocytes, but not in testis; Aplectin-5 was also expressed in fatbody. Ap C-type lectin 1 was, on the contrary, highly expressed in testis and also in fatbody, but not in hemocytes. Unlike ApβGRP and Aplectin-5, Ap C-type lectin 1 was not induced by Gram-positive bacteria. The results suggest that the cloned lectins may have different functions in different tissues of A. pernyi. |
| first_indexed | 2024-12-23T11:35:45Z |
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| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-12-23T11:35:45Z |
| publishDate | 2012-10-01 |
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| series | Insects |
| spelling | doaj.art-eefb85d45a1149498cf7347c50896c732022-12-21T17:48:39ZengMDPI AGInsects2075-44502012-10-01341093110410.3390/insects3041093cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyiWenli LiSuyun FangYuan LiFengjuan LiOlle TereniusPattern recognition receptors play an important role in insect immune defense. We cloned the β-1,3-glucan recognition protein, lectin-5 and C-type lectin 1 genes of Antheraea pernyi and examined the expression profiles of immune-stimulated pupae. After infection with Bacillus subtilis, Escherichia coli, Antheraea pernyi nuclear polyhedrosis virus (ApNPV) and Saccharomyces cerevisiae, respectively, the pupae showed different gene expression levels in the different tissues examined (midgut, fatbody, epidermis, testis, and hemocytes). ApβGRP and Aplectin-5 was induced by all the microorganisms, and mainly in epidermis and hemocytes, but not in testis; Aplectin-5 was also expressed in fatbody. Ap C-type lectin 1 was, on the contrary, highly expressed in testis and also in fatbody, but not in hemocytes. Unlike ApβGRP and Aplectin-5, Ap C-type lectin 1 was not induced by Gram-positive bacteria. The results suggest that the cloned lectins may have different functions in different tissues of A. pernyi.http://www.mdpi.com/2075-4450/3/4/1093insect immunityAntheraea pernyi&#946GRPlectingene expression |
| spellingShingle | Wenli Li Suyun Fang Yuan Li Fengjuan Li Olle Terenius cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi Insects insect immunity Antheraea pernyi &#946 GRP lectin gene expression |
| title | cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi |
| title_full | cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi |
| title_fullStr | cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi |
| title_full_unstemmed | cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi |
| title_short | cDNA Cloning and Expression Analysis of Pattern Recognition Proteins from the Chinese Oak Silkmoth, Antheraea pernyi |
| title_sort | cdna cloning and expression analysis of pattern recognition proteins from the chinese oak silkmoth antheraea pernyi |
| topic | insect immunity Antheraea pernyi &#946 GRP lectin gene expression |
| url | http://www.mdpi.com/2075-4450/3/4/1093 |
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