Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate
An in vitro gas production (GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract (SB) with exogenous fibrolytic enzymes (EZ) based on xylanase (X) and cellulase (C), or their mixture (XC; 1:1 v/v) on in vitro fermentation characteristics of a tot...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913607327 |
| _version_ | 1818901214586208256 |
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| author | Abdelfattah Z M Salem German Buendía-Rodríguez Mona M M Elghandour María A Mariezcurrena Berasain Francisco J Peña Jiménez Alberto B Pliego Juan C V Chagoyán María A Cerrillo Miguel A Rodríguez |
| author_facet | Abdelfattah Z M Salem German Buendía-Rodríguez Mona M M Elghandour María A Mariezcurrena Berasain Francisco J Peña Jiménez Alberto B Pliego Juan C V Chagoyán María A Cerrillo Miguel A Rodríguez |
| author_sort | Abdelfattah Z M Salem |
| collection | DOAJ |
| description | An in vitro gas production (GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract (SB) with exogenous fibrolytic enzymes (EZ) based on xylanase (X) and cellulase (C), or their mixture (XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture (50:50, w/w) as substrate. Four levels of SB (0, 0.6, 1.2 and 1.8 mL g−1 dry matter (DM)) and four supplemental styles of EZ (1 μL g−1 DM; control (no enzymes), X, C and XC (1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP (mL g−1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant pH was determined, and then filtered to determine dry matter degradability (DMD). Fermentation parameters, such as the 24 h gas yield (GY24), in vitro organic matter digestibility (OMD), metabolizable energy (ME), short chain fatty acid concentrations (SCFA), and microbial crude protein production (MCP) were also estimated. Results indicated that there was a SB×EZ interaction (P<0.0001) for the asymptotic gas production (b), the rate of gas production (c), GP from 6 to 72 h, GP2 (P=0.0095), and GP4 (P=0.02). The SB and different combination of enzymes supplementation influenced (P<0.001) in vitro GP parameters after 12 h of incubation; the highest doses of SB (i.e., 1.8 mL g−1 DM), in the absence of any EZ, quadratically increased (P<0.05) the initial delay before GP begins (L) and GP at different incubation times, with lowering b (quadratic effect, P<0.0001) and c (quadratic effect, P<0.0001; linear effect, P=0.0018). The GP was the lowest (P<0.05) when the highest SB level was combined with cellulose. There were SB×EZ interactions (P<0.001) for OMD, ME, the partitioning factor at 72 h of incubation (PF72), GY24, SCFA, MCP (P=0.0143), and pH (P=0.0008). The OMD, ME, GY24 and SCFA with supplementation of SB extract at 1.8 mL g−1 DM were higher (P<0.001) than the other treatments, however, PF72 was lower (quadratic effect, P=0.0194) than the other levels. Both C and X had no effect (P>on OMD, pH, ME, GY24, SCFA and MP. The combination of SB with EZ increased (P<0.001) OMD, ME, SCFA, PF72 and GP24, whereas there was no impact on pH. It could be concluded that addition of SB extract, C, and X effectively improved the in vitro rumen fermentation, and the combination of enzyme with SB extract at the level of 1.2 mL g−1 was more effective than the other treatments. |
| first_indexed | 2024-12-19T20:16:12Z |
| format | Article |
| id | doaj.art-eefc88f20d0247629abe8f9863d407e4 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T20:16:12Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-eefc88f20d0247629abe8f9863d407e42022-12-21T20:07:10ZengElsevierJournal of Integrative Agriculture2095-31192015-01-01141131139Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrateAbdelfattah Z M Salem0German Buendía-Rodríguez1Mona M M Elghandour2María A Mariezcurrena Berasain3Francisco J Peña Jiménez4Alberto B Pliego5Juan C V Chagoyán6María A Cerrillo7Miguel A Rodríguez8Faculty of Veterinary Medicine and Animal Science, Autonomous University of the State of Mexico, Toluca P.O. 50000, Mexico; Correspondence Abdelfattah Z M Salem, Tel/Fax: +1-521-7162695171National Center of Disciplinary Research in Animal Physiology and Improvement, INIFAP Ajuchitlán, Querétaro 76280, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of the State of Mexico, Toluca P.O. 50000, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of the State of Mexico, Toluca P.O. 50000, MexicoInstitute of Agricultural Sciences, Autonomous University of Hidalgo, Ex-Hda. of Aquetzalpa, Tulancingo, Hidalgo P.O. 43600, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of the State of Mexico, Toluca P.O. 50000, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of the State of Mexico, Toluca P.O. 50000, MexicoFaculty of Veterinary Medicine and Animal Science, Juárez University of the Durango State, Durango, P.O. 34280, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of the State of Mexico, Toluca P.O. 50000, MexicoAn in vitro gas production (GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract (SB) with exogenous fibrolytic enzymes (EZ) based on xylanase (X) and cellulase (C), or their mixture (XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture (50:50, w/w) as substrate. Four levels of SB (0, 0.6, 1.2 and 1.8 mL g−1 dry matter (DM)) and four supplemental styles of EZ (1 μL g−1 DM; control (no enzymes), X, C and XC (1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP (mL g−1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant pH was determined, and then filtered to determine dry matter degradability (DMD). Fermentation parameters, such as the 24 h gas yield (GY24), in vitro organic matter digestibility (OMD), metabolizable energy (ME), short chain fatty acid concentrations (SCFA), and microbial crude protein production (MCP) were also estimated. Results indicated that there was a SB×EZ interaction (P<0.0001) for the asymptotic gas production (b), the rate of gas production (c), GP from 6 to 72 h, GP2 (P=0.0095), and GP4 (P=0.02). The SB and different combination of enzymes supplementation influenced (P<0.001) in vitro GP parameters after 12 h of incubation; the highest doses of SB (i.e., 1.8 mL g−1 DM), in the absence of any EZ, quadratically increased (P<0.05) the initial delay before GP begins (L) and GP at different incubation times, with lowering b (quadratic effect, P<0.0001) and c (quadratic effect, P<0.0001; linear effect, P=0.0018). The GP was the lowest (P<0.05) when the highest SB level was combined with cellulose. There were SB×EZ interactions (P<0.001) for OMD, ME, the partitioning factor at 72 h of incubation (PF72), GY24, SCFA, MCP (P=0.0143), and pH (P=0.0008). The OMD, ME, GY24 and SCFA with supplementation of SB extract at 1.8 mL g−1 DM were higher (P<0.001) than the other treatments, however, PF72 was lower (quadratic effect, P=0.0194) than the other levels. Both C and X had no effect (P>on OMD, pH, ME, GY24, SCFA and MP. The combination of SB with EZ increased (P<0.001) OMD, ME, SCFA, PF72 and GP24, whereas there was no impact on pH. It could be concluded that addition of SB extract, C, and X effectively improved the in vitro rumen fermentation, and the combination of enzyme with SB extract at the level of 1.2 mL g−1 was more effective than the other treatments.http://www.sciencedirect.com/science/article/pii/S2095311913607327cellulasedegradabilitygas productionSalix babylonicaxylanase |
| spellingShingle | Abdelfattah Z M Salem German Buendía-Rodríguez Mona M M Elghandour María A Mariezcurrena Berasain Francisco J Peña Jiménez Alberto B Pliego Juan C V Chagoyán María A Cerrillo Miguel A Rodríguez Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate Journal of Integrative Agriculture cellulase degradability gas production Salix babylonica xylanase |
| title | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate |
| title_full | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate |
| title_fullStr | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate |
| title_full_unstemmed | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate |
| title_short | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate |
| title_sort | effects of cellulase and xylanase enzymes mixed with increasing doses of salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate |
| topic | cellulase degradability gas production Salix babylonica xylanase |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913607327 |
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