Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis Technology
ObjectivesCondyloma acuminatum (CA) is a common sexually transmitted disease caused by human papillomavirus (HPV) infection. We established a high-throughput, simple, low-cost, and accurate HPV-typing assay (polymerase chain reaction-melting temperature [PCR-Tm] analysis) to detect HPV in CA.Materia...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.857410/full |
| _version_ | 1818213042342592512 |
|---|---|
| author | Lixia Wu Lixia Wu Weifeng Wang Weifeng Wang Jie Zhang Xuan Wu Xuan Wu Yan Chen Xiaoping Gu Huaqing Shao Hongsheng Li Weiwei Liu Weiwei Liu Weiwei Liu |
| author_facet | Lixia Wu Lixia Wu Weifeng Wang Weifeng Wang Jie Zhang Xuan Wu Xuan Wu Yan Chen Xiaoping Gu Huaqing Shao Hongsheng Li Weiwei Liu Weiwei Liu Weiwei Liu |
| author_sort | Lixia Wu |
| collection | DOAJ |
| description | ObjectivesCondyloma acuminatum (CA) is a common sexually transmitted disease caused by human papillomavirus (HPV) infection. We established a high-throughput, simple, low-cost, and accurate HPV-typing assay (polymerase chain reaction-melting temperature [PCR-Tm] analysis) to detect HPV in CA.Materials and MethodsWe detected 280 cervical scraping samples, including positive samples of HPV-6 (26), HPV-11 (12), HPV-16 (22), HPV-42 (18), HPV-43 (25), HPV-multiple (19), HPV- other type (58), and HPV-negative samples (100). All samples were compared by PCR-Tm analysis and a flow fluorescence hybridization assay. Sequencing was used to confirm the results of the PCR-Tm analysis.ResultsPCR-Tm analysis was specific for each genotype (HPV-6, HPV-11, HPV-16, HPV-42, and HPV-43). The sensitivity of the PCR-Tm analysis assay for each genotype was 103, 103, 103, 103, and 102 copies/reaction, respectively. Most of the 158 samples, including 58 HPV-other type positive and 100 HPV-negative samples tested by the flow fluorescence hybridization assay, were tested negative by PCR-Tm analysis. For the 122 remaining samples, 26 HPV-6, 12 HPV-11, 22 HPV-16, 18 HPV-42, 25 HPV-43, and 19 multiple HPV infections were detected through PCR-Tm analysis. In total, 25 HPV-6, 12 HPV-11, 21 HPV-16, 18 HPV-42, 25 HPV-43, and only 10 multiple HPV infections were detected by the flow fluorescence hybridization assay. The kappa coefficient for the analysis of PCR-Tm analysis and flow fluorescence hybridization assay was 0.940 (P < 0.0001), and the 95% confidence interval of the kappa coefficient was 90.3–97.7%.ConclusionPCR-Tm analysis enabled the detection of HPV-6, HPV-11, HPV-16, HPV-42, and HPV-43, including single and multiple infections. |
| first_indexed | 2024-12-12T05:58:00Z |
| format | Article |
| id | doaj.art-eefcd3336d5b4f50860779290f8b8d19 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T05:58:00Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-eefcd3336d5b4f50860779290f8b8d192022-12-22T00:35:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.857410857410Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis TechnologyLixia Wu0Lixia Wu1Weifeng Wang2Weifeng Wang3Jie Zhang4Xuan Wu5Xuan Wu6Yan Chen7Xiaoping Gu8Huaqing Shao9Hongsheng Li10Weiwei Liu11Weiwei Liu12Weiwei Liu13Department of Laboratory Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Children's Hospital of Soochow University, Medical College of Soochow University, Soochow, ChinaDepartment of Laboratory Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Central Laboratory, Shanghai Tenth People's Hospital, Tongji University of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Central Laboratory, Shanghai Tenth People's Hospital, Tongji University of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Central Laboratory, Shanghai Tenth People's Hospital, Tongji University of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Central Laboratory, Shanghai Tenth People's Hospital, Tongji University of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Shanghai Skin Disease Hospital, Tongji University of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Laboratory Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Central Laboratory, Shanghai Tenth People's Hospital, Tongji University of Medicine, Shanghai, ChinaDepartment of Laboratory Medicine, Shanghai Skin Disease Hospital, Tongji University of Medicine, Shanghai, ChinaObjectivesCondyloma acuminatum (CA) is a common sexually transmitted disease caused by human papillomavirus (HPV) infection. We established a high-throughput, simple, low-cost, and accurate HPV-typing assay (polymerase chain reaction-melting temperature [PCR-Tm] analysis) to detect HPV in CA.Materials and MethodsWe detected 280 cervical scraping samples, including positive samples of HPV-6 (26), HPV-11 (12), HPV-16 (22), HPV-42 (18), HPV-43 (25), HPV-multiple (19), HPV- other type (58), and HPV-negative samples (100). All samples were compared by PCR-Tm analysis and a flow fluorescence hybridization assay. Sequencing was used to confirm the results of the PCR-Tm analysis.ResultsPCR-Tm analysis was specific for each genotype (HPV-6, HPV-11, HPV-16, HPV-42, and HPV-43). The sensitivity of the PCR-Tm analysis assay for each genotype was 103, 103, 103, 103, and 102 copies/reaction, respectively. Most of the 158 samples, including 58 HPV-other type positive and 100 HPV-negative samples tested by the flow fluorescence hybridization assay, were tested negative by PCR-Tm analysis. For the 122 remaining samples, 26 HPV-6, 12 HPV-11, 22 HPV-16, 18 HPV-42, 25 HPV-43, and 19 multiple HPV infections were detected through PCR-Tm analysis. In total, 25 HPV-6, 12 HPV-11, 21 HPV-16, 18 HPV-42, 25 HPV-43, and only 10 multiple HPV infections were detected by the flow fluorescence hybridization assay. The kappa coefficient for the analysis of PCR-Tm analysis and flow fluorescence hybridization assay was 0.940 (P < 0.0001), and the 95% confidence interval of the kappa coefficient was 90.3–97.7%.ConclusionPCR-Tm analysis enabled the detection of HPV-6, HPV-11, HPV-16, HPV-42, and HPV-43, including single and multiple infections.https://www.frontiersin.org/articles/10.3389/fmicb.2022.857410/fullhuman papillomavirus (HPV)melting temperature (Tm)polymerase chain reaction (PCR)condyloma acuminatum (CA)genetic testing technology |
| spellingShingle | Lixia Wu Lixia Wu Weifeng Wang Weifeng Wang Jie Zhang Xuan Wu Xuan Wu Yan Chen Xiaoping Gu Huaqing Shao Hongsheng Li Weiwei Liu Weiwei Liu Weiwei Liu Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis Technology Frontiers in Microbiology human papillomavirus (HPV) melting temperature (Tm) polymerase chain reaction (PCR) condyloma acuminatum (CA) genetic testing technology |
| title | Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis Technology |
| title_full | Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis Technology |
| title_fullStr | Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis Technology |
| title_full_unstemmed | Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis Technology |
| title_short | Detection of Five Types of HPV Genotypes Causing Anogenital Warts (Condyloma Acuminatum) Using PCR-Tm Analysis Technology |
| title_sort | detection of five types of hpv genotypes causing anogenital warts condyloma acuminatum using pcr tm analysis technology |
| topic | human papillomavirus (HPV) melting temperature (Tm) polymerase chain reaction (PCR) condyloma acuminatum (CA) genetic testing technology |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.857410/full |
| work_keys_str_mv | AT lixiawu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT lixiawu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT weifengwang detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT weifengwang detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT jiezhang detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT xuanwu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT xuanwu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT yanchen detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT xiaopinggu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT huaqingshao detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT hongshengli detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT weiweiliu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT weiweiliu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology AT weiweiliu detectionoffivetypesofhpvgenotypescausinganogenitalwartscondylomaacuminatumusingpcrtmanalysistechnology |