A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern Italy
One serious concern associated with the SARS-CoV-2 pandemic is that the virus might spill back from humans to wildlife, which would render some animal species reservoirs of the human virus. We assessed the potential circulation of SARS-CoV-2 caused by reverse infection from humans to bats, by perfor...
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MDPI AG
2022-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/7/742 |
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| author | Hiba Dakroub Danilo Russo Luca Cistrone Francesco Serra Giovanna Fusco Esterina De Carlo Maria Grazia Amoroso |
| author_facet | Hiba Dakroub Danilo Russo Luca Cistrone Francesco Serra Giovanna Fusco Esterina De Carlo Maria Grazia Amoroso |
| author_sort | Hiba Dakroub |
| collection | DOAJ |
| description | One serious concern associated with the SARS-CoV-2 pandemic is that the virus might spill back from humans to wildlife, which would render some animal species reservoirs of the human virus. We assessed the potential circulation of SARS-CoV-2 caused by reverse infection from humans to bats, by performing bat surveillance from different sites in Central–Southern Italy. We restricted our survey to sampling techniques that are minimally invasive and can therefore be broadly applied by non-medical operators such as bat workers. We collected 240 droppings or saliva from 129 bats and tested them using specific and general primers for SARS-CoV-2 and coronaviruses, respectively. All samples (127 nasal swabs and 113 faecal droppings) were negative for SARS-CoV-2, and these results were confirmed by testing the samples with the Droplet Digital PCR. Additionally, pancoronavirus end-point RT-PCR was performed, and no sample showed specific bands. This outcome is a first step towards a better understanding of the reverse transmission of this virus to bats. Although the occurrence of a reverse zoonotic pattern can only be fully established by serological testing, the latter might represent an in-depth follow-up to a broad-scale preliminary assessment performed with our approach. We encourage the systematic surveillance of bats to help prevent reverse zoonotic episodes that would jeopardize human health, as well as biodiversity conservation and management. |
| first_indexed | 2024-03-09T13:13:40Z |
| format | Article |
| id | doaj.art-eefd557cd63f482e8af15e7a0b1631e9 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T13:13:40Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-eefd557cd63f482e8af15e7a0b1631e92023-11-30T21:39:03ZengMDPI AGPathogens2076-08172022-06-0111774210.3390/pathogens11070742A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern ItalyHiba Dakroub0Danilo Russo1Luca Cistrone2Francesco Serra3Giovanna Fusco4Esterina De Carlo5Maria Grazia Amoroso6Wildlife Research Unit, Dipartimento di Agraria, Università degli Studi di Napoli Federico II, Via Università, 100, 80055 Portici, ItalyWildlife Research Unit, Dipartimento di Agraria, Università degli Studi di Napoli Federico II, Via Università, 100, 80055 Portici, ItalyForestry and Conservation, Via Botticelli, 14, 03043 Cassino, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute,2, 80055 Portici, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute,2, 80055 Portici, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute,2, 80055 Portici, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute,2, 80055 Portici, ItalyOne serious concern associated with the SARS-CoV-2 pandemic is that the virus might spill back from humans to wildlife, which would render some animal species reservoirs of the human virus. We assessed the potential circulation of SARS-CoV-2 caused by reverse infection from humans to bats, by performing bat surveillance from different sites in Central–Southern Italy. We restricted our survey to sampling techniques that are minimally invasive and can therefore be broadly applied by non-medical operators such as bat workers. We collected 240 droppings or saliva from 129 bats and tested them using specific and general primers for SARS-CoV-2 and coronaviruses, respectively. All samples (127 nasal swabs and 113 faecal droppings) were negative for SARS-CoV-2, and these results were confirmed by testing the samples with the Droplet Digital PCR. Additionally, pancoronavirus end-point RT-PCR was performed, and no sample showed specific bands. This outcome is a first step towards a better understanding of the reverse transmission of this virus to bats. Although the occurrence of a reverse zoonotic pattern can only be fully established by serological testing, the latter might represent an in-depth follow-up to a broad-scale preliminary assessment performed with our approach. We encourage the systematic surveillance of bats to help prevent reverse zoonotic episodes that would jeopardize human health, as well as biodiversity conservation and management.https://www.mdpi.com/2076-0817/11/7/742batscoronavirusesSARS-CoV-2spillbackzoonotic viruses |
| spellingShingle | Hiba Dakroub Danilo Russo Luca Cistrone Francesco Serra Giovanna Fusco Esterina De Carlo Maria Grazia Amoroso A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern Italy Pathogens bats coronaviruses SARS-CoV-2 spillback zoonotic viruses |
| title | A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern Italy |
| title_full | A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern Italy |
| title_fullStr | A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern Italy |
| title_full_unstemmed | A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern Italy |
| title_short | A First Assessment of SARS-CoV-2 Circulation in Bats of Central–Southern Italy |
| title_sort | first assessment of sars cov 2 circulation in bats of central southern italy |
| topic | bats coronaviruses SARS-CoV-2 spillback zoonotic viruses |
| url | https://www.mdpi.com/2076-0817/11/7/742 |
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